Stimulation and Inhibition With Lh and Other Hormones of Female Mammalian Meiosis in Vitro

Cows are not mice: The role of cyclic AMP, phosphodiesterases, and adenosine monophosphate‐activated protein kinase in the maintenance of meiotic arrest in bovine oocytes

“…An early attempt to induce maturation of cow and sow oocytes in intact follicles in vitro with LH or FSH was not successful (Foote and Thibault, 1969); successful induction of meiosis in oocytes from livestock species cultured in intact follicle has, to our knowledge, never been reported. In a later study, however, LH, hCG, cAMP, PGE 1 , and PGE 2 decreased meiotic maturation of cow oocytes (cultured without follicles) in one of the two culture media tested (Jagiello et al, 1975). In addition to the different effects of the cAMP‐elevating agents in different culture media, cAMP itself was reported as being “ineffective, inhibitory or stimulatory depending on the species” [ewe, cow, monkey, and human oocytes were tested by Jagiello et al (1975)].…”

“…In a later study, however, LH, hCG, cAMP, PGE 1 , and PGE 2 decreased meiotic maturation of cow oocytes (cultured without follicles) in one of the two culture media tested (Jagiello et al, 1975). In addition to the different effects of the cAMP‐elevating agents in different culture media, cAMP itself was reported as being “ineffective, inhibitory or stimulatory depending on the species” [ewe, cow, monkey, and human oocytes were tested by Jagiello et al (1975)]. In other studies, mouse and rat oocyte meiosis was almost totally inhibited by dbcAMP at 100 µg/ml (Stern and Wassarman, 1974; Magnusson and Hillensjö, 1977), while a concentration of 750 µg/ml was necessary to increase the percentage of bovine oocytes remaining at the GV stage (Jagiello et al, 1981).…”

Hammond Memorial Lecture

“…It is clear from many studies ( Table 3) that while the majority of the oocytes resume meiosis, as indicated by GVB, only a variable proportion reach metaphase II. This type of screening does not necessarily yield any valuable conclusions (Jagiello, Ducayen, Miller, Graffeo & Fang, 1975). This type of screening does not necessarily yield any valuable conclusions (Jagiello, Ducayen, Miller, Graffeo & Fang, 1975).…”

“…This general observation has stimulated many attempts to discover, among polypeptide and steroid hormones, prostaglandins and cyclic AMP, those which induce the resumption of meiosis. Donahue, 1968 Erickson & Sorensen, 1974Erickson & Ryan, 1976Yanagimachi, 1974 Haidri Thibault & Gérard, 1971Tsafriri & Channing, 1975a, b Shea et al, 1976Cho & Lim, 1975Hunter et al, 1972Thibault étal., 1975bQuirke & Gordon, 1971 Mahi Suzuki & Mastroianni, 1966Jagiello et al, 1975Thibault et al, 1977Shea étal, 1975 Kennedy & Donahue, 1969Jagiello étal, 1975 In fact, three situations involving specific oocyte stages have been used. Donahue, 1968 Erickson & Sorensen, 1974Erickson & Ryan, 1976Yanagimachi, 1974 Haidri Thibault & Gérard, 1971Tsafriri & Channing, 1975a, b Shea et al, 1976Cho & Lim, 1975Hunter et al, 1972Thibault étal., 1975bQuirke & Gordon, 1971 Mahi Suzuki & Mastroianni, 1966Jagiello et al, 1975Thibault et al, 1977Shea étal, 1975 Kennedy & Donahue, 1969Jagiello étal, 1975 In fact, three situations involving specific oocyte stages have been used.…”

The Control of Meiotic Arrest and Resumption in Mammalian Oocytes

“…As in rodent oocytes, several early studies showed that cAMP-elevating agents could decrease meiotic maturation of bovine cumulus-enclosed oocytes (Jagiello et al, 1975(Jagiello et al, , 1981. However, higher concentrations of the cAMP-elevating agents were necessary to transiently increase the percentage of bovine oocytes remaining at the GV stage after in vitro culture (Jagiello et al, 1981, Sirard & First, 1988 compared to concentrations that were effective in rodent oocytes.…”