Stimulating effects of reduced graphene oxide on the growth and nitrogen fixation activity of nitrogen-fixing bacterium Azotobacter chroococcum

Ouyang, Peng; Liang, Chengzhuang; Liu, Fangshi; Chen, Qian; Yan, Ziqiao; Ran, Junyao; Mou, Shiyu; Yuan, Yue; Wu, Xian; Yang, Shengnan

doi:10.1016/j.chemosphere.2022.133702

Cited by 10 publications

(3 citation statements)

References 54 publications

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“…Although the nitrogen fixation pathways of OA-G20 were not completely understood, the current study depicts that the nitrogen fixation pathway is activated upon the graphene exposure. This result corroborates the previous report on the enhanced nitrogen fixation and growth of Azotobacter chroococcum by reduced graphene oxide 40 . This finding highlights the stimulating effects of graphene on nitrogen fixation and the eco-friendly aspects of graphene with minimal bactericidal properties.…”

Section: Resultssupporting

confidence: 93%

Transcriptome-wide marker gene expression analysis of stress-responsive sulfate-reducing bacteria

Jawaharraj,

Peta,

Dhiman

et al. 2023

Sci Rep

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Sulfate-reducing bacteria (SRB) are terminal members of any anaerobic food chain. For example, they critically influence the biogeochemical cycling of carbon, nitrogen, sulfur, and metals (natural environment) as well as the corrosion of civil infrastructure (built environment). The United States alone spends nearly $4 billion to address the biocorrosion challenges of SRB. It is important to analyze the genetic mechanisms of these organisms under environmental stresses. The current study uses complementary methodologies, viz., transcriptome-wide marker gene panel mapping and gene clustering analysis to decipher the stress mechanisms in four SRB. Here, the accessible RNA-sequencing data from the public domains were mined to identify the key transcriptional signatures. Crucial transcriptional candidate genes of Desulfovibrio spp. were accomplished and validated the gene cluster prediction. In addition, the unique transcriptional signatures of Oleidesulfovibrio alaskensis (OA-G20) at graphene and copper interfaces were discussed using in-house RNA-sequencing data. Furthermore, the comparative genomic analysis revealed 12,821 genes with translation, among which 10,178 genes were in homolog families and 2643 genes were in singleton families were observed among the 4 genomes studied. The current study paves a path for developing predictive deep learning tools for interpretable and mechanistic learning analysis of the SRB gene regulation.

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Section: Resultssupporting

confidence: 93%

Transcriptome-wide marker gene expression analysis of stress-responsive sulfate-reducing bacteria

Jawaharraj,

Peta,

Dhiman

et al. 2023

Sci Rep

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“…Nutrients attached to some graphene materials such as reduced graphene oxide (rGO), might promote not only bacterial growth but also deactivation of rGO by oxidation to graphene oxide (GO) (Guo et al, 2017 ). It was also proposed that enhanced growth of the free‐living nitrogen‐fixing bacterium Azotobacter chroococcum in the presence of rGO could have been a result of an increase in extracellular electron transfer (Ouyang et al, 2022 ). In contrast to these findings, Akhavan and Ghaderi ( 2012 ) reported that the reduction of GO sheets by Escherichia coli resulted in the inhibition of bacterial proliferation on the surface of the reduced sheets.…”

Section: Discussionmentioning

confidence: 99%

“…For example, Yilihamu et al ( 2020 ) showed that GO can be toxic to free‐living nitrogen‐fixing bacterium, Azotobacter chroococcum in a concentration‐dependent manner. In contrast to this, Ouyang et al ( 2022 ) reported that reduced GO (rGO) has positive effects on A. chroococcum , wherein rGO was shown to stimulate bacterial cell growth and nitrogenase activity. These findings are in agreement with other studies where some graphene materials were shown to promote bacterial growth and biofilm formation (Ruiz et al, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Effect of graphene on soybean root colonization by Bradyrhizobium strains

Sethu Madhavan,

Montanez Hernandez,

et al. 2023

Plant Direct

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Legume crops such as soybean obtain a large portion of their nitrogen nutrition through symbiotic nitrogen fixation by diazotrophic rhizobia bacteria in root nodules. However, nodule occupancy by low‐capacity nitrogen‐fixing rhizobia can lead to lower‐than‐optimal levels of nitrogen fixation. Seed/root coating with engineered materials such as graphene‐carrying biomolecules that may promote specific attraction/attachment of desirable bacterial strains is a potential strategy that can help overcome this rhizobia competition problem. As a first step towards this goal, we assessed the impact of graphene on soybean and Bradyrhizobium using a set of growth, biochemical, and physiological assays. Three different concentrations of graphene were tested for toxicity in soybean (50, 250, and 1,000 mg/l) and Bradyrhizobia (25, 50, and 100 mg/l). Higher graphene concentrations (250 mg/l and 1,000 mg/l) promoted seed germination but slightly delayed plant development. Spectrometric and microscopy assays for hydrogen peroxide and superoxide anion suggested that specific concentrations of graphene led to higher levels of reactive oxygen species in the roots. In agreement, these roots also showed higher activities of antioxidant enzymes, catalase, and ascorbate peroxidase. Conversely, no toxic effects were detected on Bradyrhizobia treated with graphene, and neither did they have higher levels of reactive oxygen species. Graphene treatments at 250 mg/l and 1,000 mg/l significantly reduced the number of nodules, but rhizobia infection and the overall nitrogenase activity were not affected. Our results show that graphene can be used as a potential vehicle for seed/root treatment.

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Graphene increased the richness and diversity of bacterial community in Cd-polluted Haplic Cambisols in a time-dependent manner

Zhang

Liu

et al. 2023

J Soils Sediments

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Stimulating effects of reduced graphene oxide on the growth and nitrogen fixation activity of nitrogen-fixing bacterium Azotobacter chroococcum

Cited by 10 publications

References 54 publications

Transcriptome-wide marker gene expression analysis of stress-responsive sulfate-reducing bacteria

Transcriptome-wide marker gene expression analysis of stress-responsive sulfate-reducing bacteria

Effect of graphene on soybean root colonization by Bradyrhizobium strains

Graphene increased the richness and diversity of bacterial community in Cd-polluted Haplic Cambisols in a time-dependent manner

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