Stimulated Raman spectroscopic imaging by microsecond delay-line tuning

Broadband Coherent Raman Scattering Microscopy

“…Recently, efforts have been devoted to increase the wavelength tuning speed, using rapid scanning optical delay lines whose designs have been borrowed from optical coherence tomography . Liao et al . used a 12 kHz resonant mirror, similar to that used by Hashimoto et al.…”

Quantitative imaging of lipid droplets in single cells

“…For example, Liao et al demonstrated parallel acquisition of SRS signal over 180 cm −1 bandwidth (~20 spectral data points) with 42 μs pixel dwell time using spectrally focused laser pulses and a homebuilt microsecond optical delay-line tuner. 127 He et al integrated a galvanometer mirror-based rapid-scanning optical delay line with spectrally focused laser pulses to acquire a spectrum with 20 data points in 40 μs. 128 Liao et al built an array of tuned amplifiers for lock-in free parallel acquisition of SRS signal over 180 cm −1 bandwidth (~20 spectral data points) with 32 μs pixel dwell time using multiplexed SRS.…”

Ultra-multiplex CARS spectroscopic imaging with 1-millisecond pixel dwell time

“…The use of spectroscopic imaging has become prominent for coherent or nonlinear Raman imaging methods [1][2][3][4][5][6][7][8][9], among which coherent anti-Stokes Raman scattering (CARS) and stimulated Raman scattering (SRS) are two methods that are often used. Based on the CARS and SRS imaging techniques, several kinds of spectroscopic imaging have been reported, such as the multiplex method [10][11][12][13][14][15][16], laser-wavelength-scanning method [17][18][19], spectral focusing method [20], FT-CARS [21][22][23], and dual-comb method [24]. The multiplex method [10][11][12][13][14] utilizes a multi-channel detector such as a CCD or C-MOS camera with a spectrometer to cover the broad spectral range of the coherent Raman signal.…”