Stimulated Raman Scattering Microscopy Enables Gleason Scoring of Prostate Core Needle Biopsy by a Convolutional Neural Network

Abstract

Focal therapy (FT) has been proposed as an approach to eradicate clinically significant prostate cancer (PCa) while preserving the normal surrounding tissues to minimize treatment-related toxicity. Rapid histology of core needle biopsies is essential to ensure the precise FT for localized lesions and to determine tumor grades. However, it is difficult to achieve both high accuracy and speed with currently available histopathology methods. Here, we demonstrated that stimulated Raman scattering (SRS) microscopy could reveal the largely heterogeneous histologic features of fresh prostatic biopsy tissues in a label-free and near real-time manner. A diagnostic convolutional neural network (CNN) built based on images from 61 patients could classify Gleason patterns of PCa with an accuracy of 85.7%. An additional 22 independent cases introduced as external test dataset validated the CNN performance with 84.4% accuracy. Gleason scores of core-needle biopsies from 21 cases were calculated using the deep learning SRS system and showed a 71% diagnostic consistency with grading from three pathologists. This study demonstrates the potential of a deep learning-assisted SRS platform in evaluating the tumor grade of prostate cancer, which could help simplify the diagnostic workflow and provide timely histopathology compatible with FT treatment.

Photoswitchable vibrational nanoscopy with sub-100-nm optical resolution

“…Apart from the experimental improvements, image denoising might be an additional choice to increase the SNR, owing to the rapid development of deep-learning algorithms 39 – 42 Furthermore, as another powerful vibration spectroscopy, IR absorption coupled with photochromic probes may demonstrate interesting photoswitching capability, as shown in Fig. S9 in the Supplementary Material and could be potentially realized for SR vibrational imaging as well.…”

Scanning electron microscopy of digestive tract parasites in jungle cat (Felis chaus)

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Stain-Free Histopathology with Stimulated Raman Scattering Microscopy

“…However, urologists are concerned about its effectiveness due to the difficulties in real-time evaluation of lesions to ensure complete and safe ablation. Ao et al reported that SRS microscopy was able to effectively reveal the diverse histologic features of fresh prostatic CNB tissues. Four histologic grades, including benign and Gleason patterns 3–5, were imaged and confirmed with H&E on both thin sections and fresh tissues (Figure a).…”

“…Examples of label-free histology for prostate, gout, pancreas, and laryngeal tissues. (a) Diagnostic features of fresh prostate tissues revealed by SRS . Scale bar: 500 μm in large scale image and 50 μm in magnified images.…”