Stimulated Osteogenic Differentiation of Human Mesenchymal Stem Cells by Reduced Graphene Oxide

Jin, Linhua; Lee, Jong Ho; Jin, Oh Seong; Shin, Yong Cheol; Kim, Min Jeong; Hong, Suck Won; Lee, Mi Hee; Park, Jong Chul; Han, Dong‐Wook

doi:10.1166/jnn.2015.11223

Cited by 28 publications

(18 citation statements)

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“…Similarly, electrically conductive scaffold act as the skeleton of stem cell niche in regenerative medicine [41]. In addition, injectable calcium phosphate/hyaluronic acid microparticle system is also used for platelet delivery for bone regeneration [42] (Table 1). Graphene-based nanomaterials are also used as substrates for stem cell (SC) differentiation [43].…”

Section: Role Of Mineral Elements In Osteogenic Differentiationmentioning

confidence: 99%

Role of Calcium Bio-Minerals in Regenerative Medicine and Tissue Engineering

Upadhyay¹

2017

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Present review article emphasize role of biominerals in regenerative medicine and tissue engineering. Among all biominerals calcium is essential for body growth and development. It also performs many fundamental functions in cellular metabolism. Inside cell organic matrix is calcified by calcium phosphate minerals. It also embeds bone cells which participate in the maintenance and organization of bone. This article also emphasizes use of hydroxyapatite a natural mineral used as a bone-building supplement with superior absorption in comparison to calcium. It also explains use of scaffolds that mimic the structure and composition of bone tissue and cells. It also signifies use of HAc microparticles or microparticles loaded with PL, superparamagnetic iron oxide nanoparticles, composite scaffolds of nano-hydroxyapatite (nHAp) and silk fibroin (SF) in bone regeneration mainly in osteoregenerative therapy. For better and successful bone regeneration there is a need to develop low cost sintered hydroxyfluorapatite discs to support cellular proliferation and colonization, tailored mineralization, cell and drug delivery. All adhesion components should show low immunoreactivity and high biocompatibility with natural bone tissues. There is an essential need to make new biocompatible materials for scaffolding, biomeinerals and cementing formulations for regeneration of bones, craniomaxillofacial, dental and orthopedic surgery.

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Section: Role Of Mineral Elements In Osteogenic Differentiationmentioning

confidence: 99%

Role of Calcium Bio-Minerals in Regenerative Medicine and Tissue Engineering

Upadhyay¹

2017

JSRT

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“…Several studies reported that RGO can induce osteogenic differentiation individually. For example, in a study by Jin et al, 58 the amount of ALP synthesized after 14 days and mineralized nodule after 21 days by the cells on RGO NPs was found to be significantly higher than those of the other groups without RGO NPs. Indeed, π-electron cloud of graphene and its derivatives cause interactions with the inner hydrophobic core of proteins.…”

Section: Graphene and Its Derivatives' Properties Affecting Osteogenesismentioning

confidence: 90%

“…85 RGO NPs also showed a dose-dependent decrease in cell viability, as concentrations >60 μg/mL seemed to reduce viability. 58,110 In a study by Mehrali M et al, 86 1.5 wt % RGO-containing composite displayed the most viable cells compared to CS with 1.0, 0.75, 0.5, 0.25, 0 wt % RGO.…”

Section: Concentration Of Graphenementioning

confidence: 97%

“…The most dominant cell types used in the included articles were mesenchymal stem cells (MSCs), including human adipose-derived stem cells (hADSCs), [17][18][19][20][21][22] Goat ADSCs, 23 human dental pulp stem cells (hDPSCs), 24 human alveolar bone marrow stem cells (hABMSCs), 25 human periodontal ligament stem cells (hPDLSCs), 26,27 and bone MSCs (BMSCs), [28][29][30][31] such as human BMSCs 19, 32-41 (hBMSCs), murine BMSCs (mBMSCs), 4, 42-49 rabbit BMSCs (rBMSCs), 50 murine MSCs cell line C3H10T1/2 51,52 and Caprine BMSCs (cMBSCs). 53 The type of the MSCs in fifteen studies 19,32,36,40,[54][55][56][57][58][59][60][61][62][63][64] was not defined. Sixteen studies used preosteoblast cells, 14,[65][66][67][68][69][70][71][72][73][74]…”

Section: Cell Sourcesmentioning

confidence: 99%

“…In addition, MTT assay performed in 21 included studies was discovered to be not successful in predicting the toxicity of graphene and its derivatives due to the spontaneous reduction of MTT by them, leading to a false positive signal. 100 Therefore, to evaluate the toxicity of graphene, it is much better to use alternate assays, such as water-soluble tetrazolium salt (WST-8) assay, 58 ROS assay, 41 CCK-8 assay 52,76,77,79 and DNA counting 25, 36, 37, 71 3. In nine included studies the type of the MSCs, and in one study, type of the preosteoblast cells were not defined.…”

Section: Limitationsmentioning

confidence: 99%

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In vitro effect of graphene structures as an osteoinductive factor in bone tissue engineering: A systematic review

Mohammadrezaei

Golzar

Rad

et al. 2018

J Biomedical Materials Res

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Graphene and its derivatives have been well-known as influential factors in differentiating stem/progenitor cells toward the osteoblastic lineage. However, there have been many controversies in the literature regarding the parameters effect on bone regeneration, including graphene concentration, size, type, dimension, hydrophilicity, functionalization, and composition. This study attempts to produce a comprehensive review regarding the given parameters and their effects on stimulating cell behaviors such as proliferation, viability, attachment and osteogenic differentiation. In this study, a systematic search of MEDLINE database was conducted for in vitro studies on the use of graphene and its derivatives for bone tissue engineering from January 2000 to February 2018, organized according to the PRISMA statement. According to reviewed articles, different graphene derivative, including graphene, graphene oxide (GO) and reduced graphene oxide (RGO) with mass ratio ≤1.5 wt % for all and concentration up to 50 μg/mL for graphene and GO, and 60 μg/mL for RGO, are considered to be safe for most cell types. However, these concentrations highly depend on the types of cells. It was discovered that graphene with lateral size less than 5 µm, along with GO and RGO with lateral dimension less than 1 µm decrease cell viability. In addition, the three-dimensional structure of graphene can promote cell-cell interaction, migration and proliferation. When graphene and its derivatives are incorporated with metals, polymers, and minerals, they frequently show promoted mechanical properties and bioactivity. Last, graphene and its derivatives have been found to increase the surface roughness and porosity, which can highly enhance cell adhesion and differentiation. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2284-2343, 2018.

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Development of Graphene‐Based Materials in Bone Tissue Engineaering

et al. 2021

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Currently, general treatments of criticalsized bone defects include autografts, allografts, and the use of bone regeneration biomaterials. [1] Autograft is the gold standard approach due to its satisfying osteogenicity. But it also faces drawbacks such as an invasive surgical operation for bone harvest and scarcity of available donor sites. [2] Allograft is accompanied by the risk of disease transmission and immunological rejection. [2] Therefore, significant efforts have been devoted to finding alternative approaches for bone regeneration. Bone tissue engineering (BTE) provides a promising alternative strategy to realize higher-level restoration of bone defects. BTE exploits the elaborate combination of scaffolds, seeding cells, and biological factors to form a functional substitute or assist in situ regeneration. In most BTE research, stem cells are implanted into defective bone sites with the support of a biomaterial-based scaffold and are supposed to proliferate and differentiate into osteoblasts to promote bone regeneration. However, the performances of many current BTE systems could not meet the therapeutic expectation due to problems such as insufficient osteogenic differentiation and limited vascularization. [3][4][5] Many studies tried to improve the performance of BTE via enhancing the properties of the biomaterial-based scaffold. [6] In addition to traditional macroor microscale forms of metals, ceramics, and polymers, their nanoscale counterparts have been extensively explored for BTE due to their unique properties. [7][8][9][10] Ever since Andre Geim and Konstantin Novoselov [11] discovered a simple and feasible method for graphene isolation, graphene family materials (GFMs) have attracted great interest in regenerative medicine and tissue engineering. In BTE, bGBMs show promising potentials such as mechanical strength, high specific surface, and adsorption ability. [12][13][14][15][16][17] According to the composition, bGBMs could be categorized into GFMs and GFMs-containing composite materials (GCMs). GFMs refer to graphene and its derivatives, containing 0D graphene quantum dots (GQDs), 2D graphene (GR), graphene oxide (GO), and reduced graphene oxide (rGO), 3D graphite, etc. Among them, GQDs, GR, GO, and rGO could be obtained by chemical approaches using graphite as the starting material. GCMs are composite materials consisting of GFMs and other materials such Bone regeneration-related graphene-based materials (bGBMs) are increasingly attracting attention in tissue engineering due to their special physical and chemical properties. The purpose of this review is to quantitatively analyze mass academic literature in the field of bGBMs through scientometrics software CiteSpace, to demonstrate the rules and trends of bGBMs, thus to analyze and summarize the mechanisms behind the rules, and to provide clues for future research. First, the research status, hotspots, and frontiers of bGBMs are analyzed in an intuitively and vividly visualized way. Next, the extracted important subjects such as fabricatio...

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Stimulated Osteogenic Differentiation of Human Mesenchymal Stem Cells by Reduced Graphene Oxide

Cited by 28 publications

References 0 publications

Role of Calcium Bio-Minerals in Regenerative Medicine and Tissue Engineering

Role of Calcium Bio-Minerals in Regenerative Medicine and Tissue Engineering

In vitro effect of graphene structures as an osteoinductive factor in bone tissue engineering: A systematic review

Development of Graphene‐Based Materials in Bone Tissue Engineaering

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