Stigmasterol inhibits lipopolysaccharide-induced innate immune responses in murine models

Antwi, Aaron Opoku; Obiri, David Darko; Osafo, Newman; Forkuo, Arnold Donkor; Essel, Leslie Brian

doi:10.1016/j.intimp.2017.10.018

Cited by 49 publications

(31 citation statements)

References 39 publications

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“…Stigmasterol has been described to have anti-osteoarthritic properties with inhibition of pro-inflammatory and matrix degradation mediators involved in cartilage degradation in part via the NF-κB pathway [100]. In LPSinduced innate immune responses in mice, stigmasterol has also been reported to decrease fever response, lung inflammation, transaminase activities and liver damages [101].…”

Section: Anti-inflammatory Activitiesmentioning

confidence: 99%

Microalgal carotenoids and phytosterols regulate biochemical mechanisms involved in human health and disease prevention

et al. 2019

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HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

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Section: Anti-inflammatory Activitiesmentioning

confidence: 99%

Microalgal carotenoids and phytosterols regulate biochemical mechanisms involved in human health and disease prevention

et al. 2019

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“…Stigmasterol is a steroid alcohol with immune-modulatory properties either alone or as a component of phytosterol mixtures [31]. It was reported to attenuate both innate and adaptive immune responses, and inhibit in ammatory cell recruitment and oxidative stress as well [15,32]. Rhein a major component of many medicinal herbs with various properties, including anti-in ammatory, antioxidant and anticancer activities [33][34][35].…”

Section: Discussionmentioning

confidence: 99%

Exploring the Pharmacological Mechanism of Cassiae Semen a Cting on Cataracts Based on a Network Pharmacology Approach

Fang¹

2020

Preprint

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BackgroundCassiae Semen (CS) is one of the most well-known herbs used in the treatment of cataracts in China. However, the potential mechanisms of its anti-cataracts effects have not been fully explored.MethodThe active compounds of CS were obtained from TCMSP database, and their targets were retrieved from the TCMSP, STITCH and DrugBank databases. Cataracts related target genes were identified from the GeneCard, Malacard, and OMIM databases. GO and KEGG analysis were performed using DAVID online tools, and Cytoscape were used to construct compound-targets network and protein-protein interaction (PPI) networks, cluster analysis were carried out using MCODE plugin for Cytoscape.ResultsWe obtained 13 active compounds from CS and 105 targets in total to construct a compound-target network, which indicated that emodin, stigmastero, and rhein served as the main ingredients in CS. A total of 238 cataracts related targets were identified from public databases. PPI networks of compound targets and cataract-related targets were constructed and merged to obtained the central network, enrichment analysis showed 50 key targets in the central network enriched in several important signaling pathways, such as thyroid hormone signaling pathway, MAPK signaling pathway, PI3K-Akt signaling pathway. The top 4 genes with higher degree in the central network were TP53, HSP90, ESR1, EGFR, indicating their important roles in the treatment of cataracts.ConclusionsThe present study systematically revealed the multi-target mechanisms of CS on cataracts using network pharmacology approach, and provided indications for further mechanistic studies and also for the development of CS as a potential treatment for cataracts patients.

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“…For example, stigmasterol was recognized as active ingredients in TKM, PN, RR and PU, while β-sitosterol was recognized in VAH, HMM, PN and PU. Recent research showed that stigmasterol inhibited LPS-induced acute liver injury with a dose-dependent manner [21]. β-Sitosterol was mentioned to decrease hepato brosis, inhibit the oxidation and in ammation against LPS/D-GalN -induced acute hepatic failure and protect against CCl4-induced hepatotoxicity in animal models [22,23].…”

Section: Discussionmentioning

confidence: 99%

Uncovering bioactive compounds and potential mechanisms of Jieduan-Niwan Formula against liver failure

Ma¹,

Zhang²,

Gao³

et al. 2020

Preprint

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Background Liver failure is considered as the inability of the liver to perform its normal synthetic and metabolic function. Our previous data showed that Jieduan-Niwan formula (JDNW) induced liver failure, while its underlying mechanism remains unknown. Methods In the present study, we performed a network pharmacology to analyze the bioactive ingredients and related targets. Compound and target data of JDNW formula were retrieved from the Traditional Chinese Medicine Systems Pharmacology Database (TCMSP), and targets related to liver diseases were obtained from DisGeNET database. Information of protein protein interaction were retrieved from STRING database. Network construction and degree calculation were used Cytoscape software. Metascape web-server was used for GO and KEGG pathway enrichment. A microarray data series (GSE72081) was obtained from Gene Expression Omnibus database and analyzed using R project and Connectivity Map. Ledock was used for molecular docking. Results A total of 114 unduplicated active ingredients were identified after ADME screening. After construction of a target-liver disease network, 28 targets were identified for anti-liver failure effect. These targets were significantly enriched in fluid shear stress and atherosclerosis, pathways in cancer, IL-17 signaling pathway, HIF-1 signaling pathway, insulin resistance, toxoplasmosis, prostate cancer, microRNAs in cancer, NF-kappa B signaling pathway, Chemical carcinogenesis, VEGF signaling pathway and complement and coagulation cascades pathways. After analyzing a public microarray data of quercetin, we found biological action of some NFκB inhibitors was similar to quercetin. Molecular docking study showed that quercetin possessed a capability to bind on IKKβ and inhibited NFκB activation. Conclusions JDNW formula treated liver failure via multiple targets and multiple pathways, including NFκB signal. Quercetin was identified as a key bioactive ingredient of JDNW formula and its anti-liver failure effect may involve in NFκB inhibition.

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Stigmasterol inhibits lipopolysaccharide-induced innate immune responses in murine models

Cited by 49 publications

References 39 publications

Microalgal carotenoids and phytosterols regulate biochemical mechanisms involved in human health and disease prevention

Microalgal carotenoids and phytosterols regulate biochemical mechanisms involved in human health and disease prevention

Exploring the Pharmacological Mechanism of Cassiae Semen a Cting on Cataracts Based on a Network Pharmacology Approach

Uncovering bioactive compounds and potential mechanisms of Jieduan-Niwan Formula against liver failure

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