Stigma of Nicotiana: Ultrastructural and Biochemical Studies

Cresti, Mauro; Keijzer, C.J.; Tiezzi, Antonio; Ciampolini, F.; Focardi, Silvano

doi:10.1002/j.1537-2197.1986.tb09702.x

Cited by 50 publications

(46 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The relative increase in extension rate was calculated by dividing the maximum extension rate by the rate just before LTP addition. tissues from solanaceous species have a loose structure with large intercellular spaces through which pollen tubes grow (Cresti et al, 1986;Kandasamy and Kristen, 1990;Hudak et al, 1993;Atkinson et al, 1995). By contrast, the cellular organization of maize styles is compact with small intercellular spaces (Kroh et al, 1979); therefore, it is more likely that a cell wall-loosening protein facilitates pollen tube growth in these types of pistils.…”

Section: Discussion Ltps and Plant Growthmentioning

confidence: 99%

Lipid Transfer Proteins Enhance Cell Wall Extension in Tobacco

et al. 2005

View full text Add to dashboard Cite

Plant cells are enclosed by a rigid cell wall that counteracts the internal osmotic pressure of the vacuole and limits the rate and direction of cell enlargement. When developmental or physiological cues induce cell extension, plant cells increase wall plasticity by a process called loosening. It was demonstrated previously that a class of proteins known as expansins are mediators of wall loosening. Here, we report a type of cell wall-loosening protein that does not share any homology with expansins but is a member of the lipid transfer proteins (LTPs). LTPs are known to bind a large range of lipid molecules to their hydrophobic cavity, and we show here that this cavity is essential for the cell wall-loosening activity of LTP. Furthermore, we show that LTP-enhanced wall extension can be described by a logarithmic time function. We hypothesize that LTP associates with hydrophobic wall compounds, causing nonhydrolytic disruption of the cell wall and subsequently facilitating wall extension.

show abstract

Section: Discussion Ltps and Plant Growthmentioning

confidence: 99%

Lipid Transfer Proteins Enhance Cell Wall Extension in Tobacco

et al. 2005

View full text Add to dashboard Cite

show abstract

“…Pollination induces many physiological and developmental responses within the flower, including ethylene evolution, stylar and perianth senescence, and changes in the nectary, ovary and ovules (Halvey, 1986;Herrero, 1992;Larsen et al, 1993;Stead, 1992;Zhang and O'Neill, 1993). Pollination also causes dramatic cellular and biochemical responses in the pistil, leading to deterioration and death in specific cell types (Cresti et al, 1986;Herrero and Dickinson, 1979;Fisher, 1968, 1969). Cell death is apparently an important aspect of pollination and fertilization.…”

Section: Introductionmentioning

confidence: 99%

Pollination induces mRNA poly(A) tail‐shortening and cell deterioration in flower transmitting tissue

Wang

Cheung³

1996

The Plant Journal

View full text Add to dashboard Cite

SummaryPollination induces many physiological responses in the flower, including deterioration and death in specific pistil cell types. It is shown here that within the style of tobacco, pollination-induced cell deterioration was restricted to the transmitting tissue while the surrounding cortical tissue was not affected. It was distinct from general senescence since exogenously applying the senescence-inducing hormone ethylene, or its precursor aminocyclopropane-1-carboxylic acid (ACC), to the flower or the pistil induced overall deterioration in the entire flower. Furthermore, both pollen tube growth and ethylene action were needed for the entire spectrum of cellular changes associated with this pollination-induced transmitting tissue deterioration process. It is also shown that pollination-induced mRNA poly(A) tail-shortening for at least three major classes of transmitting tissue-specific mRNAs. As is commonly observed for poly(A) tail-shortened mRNAs, the levels of two of these three mRNA classes decline after pollination. On the other hand, the third class of mRNAs, transmitting tissue-specific ('r'rs) mRNAs, was maintained at a very high level subsequent to pollination, even after substantial poly(A)-tail shortening. TTS mRNAs encode a pollen tube growth-promoting and -attracting protein needed for optimal in vivo pollen tube growth. The specific preservation of TTS mRNAs in the deteriorating transmitting tissue cells suggests that these cells can distinguish molecules needed in the pollinated styles from those that are dispensable, and protect them from degradation. It is suggested that the pollination-induced mRNA poly(A) tail-shortening and cell death are programmed processes suited to the post-pollination transmitting tissue environment. Results showing that ACC is a candidate signal molecule for the pollination-induced mRNA-shortening which is accentuated by ethylene and mediated via a protein phosphorylation-dependent signal transduction pathway are also presented.

show abstract

“…Therefore, understanding this process may help us follow differentiation of the secretory zone.The exu date is mainly composed of lipids, proteins and sugars (Cresti et al 1986). Since plastids are involved in lipid and starch biosynthesis, we searched for possible differ ences between plastids of wild-type and stigmaless pis tils.…”

Section: Cytoplasmic Changes During Developmentmentioning

confidence: 99%

“…Stigmas are classified as "wet" when at the receptive stage the surface is covered with a sticky exudate secret ed by the cells of the secretory zone (Cresti et al 1986;Dumas 1977;Dumas et al 1978;Heslop-Harrison and Shivanna 1977;Knox 1984;Mackenzie et al 1990). We studied pistil development in transgenic tobacco plants in which the stigma is ablated by expression of a stigmaspecific cytotoxic gene, STIG /-barnase (Goldman et al 1994).…”

Section: Introductionmentioning

confidence: 99%

Stigma development in Nicotiana tabacum. Cell death in transgenic plants as a marker to follow cell fate at high resolution

Wolters‐Arts

Derksen

Kooijman

et al. 1996

Sexual Plant Reproduction

View full text Add to dashboard Cite

bstract Pistil development was studied in transgenic bacco plants in which the stigma is ablated by expres-)ii of a stigma-specific cytotoxic gene. These plants ofr a tool to investigate the process of differentiation of .e secretory zone, in that cell death caused by barnase tivity provides a marker to follow cell fate at high reskition. After fusion of the carpel walls in the region >st distal from the ovary, the epidermal cells begin to vide in both wild-type and stigmaless plants. Divisions the LI layer of the pistil are immediately followed by : morphogenetic events that lead to three different cell les: rounded-angular cells showing an equal number of li-and periclinal divisions, cells that are more oblong fining the transition zone, and the square cells of the nsmitting tissue dividing mostly anticlinally with reect to the original carpel wall. In the stigmaless plants, II death caused by the expression of STIG /-barnase gins at stage -1 and proceeds gradually, but is always .1 sociated with round epidermal cells and with angularunded cells underneath them. Studies at the ultrastrucral level show that cell death caused by barnase activioccurs first in solitary cells and gradually extends to roups of cells. In si in hybridizations using the STIG I NA probe in wild-type pistils confirm these results, lost likely, the cells in which STIG I is expressed are lose that have just differentiated into the secretory cell pe. Our results indicate that the transition zone or neck autonomously differentiated from the secretory zone id the transmitting tissue. Furthermore, our results inditie that in both wild-type and stigmaless pistils secre>n of lipids most likely occurs through the plasmodesiata. This observation suggests that bulk transport can -cur via plasmodesmata.

show abstract

Stigma of Nicotiana: Ultrastructural and Biochemical Studies

Cited by 50 publications

References 40 publications

Lipid Transfer Proteins Enhance Cell Wall Extension in Tobacco

Lipid Transfer Proteins Enhance Cell Wall Extension in Tobacco

Pollination induces mRNA poly(A) tail‐shortening and cell deterioration in flower transmitting tissue

Stigma development in Nicotiana tabacum. Cell death in transgenic plants as a marker to follow cell fate at high resolution

Contact Info

Product

Resources

About