Stiffening of the nucleus pulposus upon axial loading of the intervertebral disc: An experimental in situ study

Beekmans, Steven V.; Emanuel, Kaj S.; Smit, T.H.; Iannuzzi, Davide

doi:10.1002/jsp2.1005

Cited by 7 publications

(6 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In recent years, bovine IVD organ culture models have gained popularity since the dimensions and biological composition of bovine IVDs is relatively equivalent to human discs [ 20 ]. Investigating the degeneration mechanism and therapeutic treatment in whole IVD explants in combination with a specific loading regime may represent a way to bridge the gap between in-vitro and in-vivo tests [ 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

One strike loading organ culture model to investigate the post-traumatic disc degenerative condition

Zhou

Cui

et al. 2021

Journal of Orthopaedic Translation

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

One strike loading organ culture model to investigate the post-traumatic disc degenerative condition

Zhou

Cui

et al. 2021

Journal of Orthopaedic Translation

View full text Add to dashboard Cite

“…On the other hand, the special anatomical structure and composition of intervertebral discs determine its specific role in forming the spine and protecting the spinal cord, which also makes it extremely difficult to recover after intervertebral disc injury, [15][16][17][18][19][20] with consequent secondary intervertebral disc degeneration, 21 fusion, 22 etc. TLBF is often complicated by an intervertebral disc injury, which can be reliably assessed by MRI.…”

Section: Discussionmentioning

confidence: 99%

Intervertebral disc injury is the mainspring for the postoperative increase in Cobb Angle after thoracolumbar burst fracture

Peng

Cui

Kuang

et al. 2022

J Orthop Surg (Hong Kong)

View full text Add to dashboard Cite

Study Design A single-institution retrospective study of a cohort of patients who underwent internal fixation spine surgery for thoracolumbar burst fracture (TLBF). Objective To observe the imaging manifestations of intervertebral disc changes in TLBF, to analyze the relationship between the degree of disc injury and the Cobb angle increase. Methods We retrospectively analyzed the data of patients who underwent short-segment pedicle screw instrumentation in the spinal surgery department of a single hospital between January 2014 and December 2017 ( n = 90). According to the magnetic resonance imaging characteristics of the superior intervertebral disc tissue of the injured vertebrae before the operation, the intervertebral disc injury was divided into three types, which was used for group allocation: group A, uninjured intervertebral disc group; group B, mild intervertebral disc injury group; and group C, severe intervertebral disc injury group. The main imaging results of the three groups Cobb, IVA, IHI, AHIV, and VAS were compared among groups. Results Ninety patients were included in the study ( n = 38, 32, and 20, in groups A, B, and C, respectively). There was no statistically significant difference in demographics among the three groups ( p > .05). 1-year post-surgery, the Cobb angle in group C differed significantly from that in groups A and B ( p < .01). There was a significant difference in Cobb angle between groups A and B after internal fixation was removed for 6 months. At 1-year post-surgery, the IHI group C differed significantly from groups A and B ( p < .01), while groups A and B were similar ( p = .102); however, at 6 months after the internal fixation was removed, the IHI differed significantly between these two groups, also the AHIV between groups A and B was statistically significant ( p < .01). The VAS pain score was similar among the three groups. Pearson’s test showed that the increase in the Cobb angle was moderately correlated with IVA and IHI, and weakly correlated with AHIV. Conclusion For TLBF with an intervertebral disc injury, the presurgical degree of intervertebral disc injury is the main reason for the post-surgery increase in the Cobb angle. Thus, diagnosis and treatment of this kind of patient require attention to the risk of spinal deformity.

show abstract

“…In recent years, bovine IVD organ culture models have gained popularity since the dimensions and biological composition of bovine IVDs is relatively equivalent to human discs 29 . Investigating the degeneration mechanism in whole IVD explants exposed to a specific loading regime may represent a way to bridge the gap between in vitro and in vivo tests 30,31 . In this study, detrimental mechanical load and/or inflammatory cytokines were applied to bovine caudal IVDs in organ culture to simulate different scenarios of early IVD degeneration.…”

Section: Introductionmentioning

confidence: 99%

“… 29 Investigating the degeneration mechanism in whole IVD explants exposed to a specific loading regime may represent a way to bridge the gap between in vitro and in vivo tests. 30 , 31 In this study, detrimental mechanical load and/or inflammatory cytokines were applied to bovine caudal IVDs in organ culture to simulate different scenarios of early IVD degeneration. The objective of this study was to use immunohistochemistry to reveal the differential distribution of neoepitopes formed due to the degradation of matrix components with the onset of ex vivo induced disc degeneration.…”

Section: Introductionmentioning

confidence: 99%

Neoepitope fragments as biomarkers for different phenotypes of intervertebral disc degeneration

Cui

Teixeira³

et al. 2022

JOR Spine

View full text Add to dashboard Cite

Background During the intervertebral disc (IVD) degeneration process, initial degenerative events occur at the extracellular matrix level, with the appearance of neoepitope peptides formed by the cleavage of aggrecan and collagen. This study aims to elucidate the spatial and temporal alterations of aggrecan and collagen neoepitope level during IVD degeneration. Methods Bovine caudal IVDs were cultured under four different conditions to mimic different degenerative situations. Samples cultured after 1‐ or 8‐days were collected for analysis. Human IVD samples were obtained from patients diagnosed with lumbar disc herniation (LDH) or adolescent idiopathic scoliosis (AIS). After immunohistochemical (IHC) staining of Aggrecanase Cleaved C‐terminus Aggrecan Neoepitope (NB100), MMP Cleaved C‐terminus Aggrecan Neoepitope (MMPCC), Collagen Type 1α1 1/4 fragment (C1α1) and Collagenase Cleaved Type I and II Collagen Neoepitope (C1,2C), staining optical density (OD)/area in extracellular matrix (OECM) and pericellular zone (OPCZ) were analyzed. Conditioned media of the bovine IVD was collected to measure protein level of inflammatory cytokines and C1,2C. Results For the bovine IVD sections, the aggrecan MMPCC neoepitope was accumulated in nucleus pulposus (NP) and cartilage endplate (EP) regions following mechanical overload in the one strike model after long‐term culture; as for the TNF‐α induced degeneration, the OECM and OPCZ of collagen C1,2C neoepitope was significantly increased in the outer AF region after long‐term culture; moreover, the C1,2C was only detected in conditioned medium from TNF‐α injection + Degenerative loading group after 8 days of culture. LDH patients showed higher MMPCC OECM in NP and higher C1,2C OECM in AF region compared with AIS patients. Conclusions In summary, aggrecan and collagen neoepitope profiles showed degeneration induction trigger‐ and region‐specific differences in the IVD organ culture models. Different IVD degeneration types are correlated with specific neoepitope expression profiles. These neoepitopes may be helpful as biomarkers of ECM degradation in early IVD degeneration and indicators of different degeneration phenotypes.

show abstract

Stiffening of the nucleus pulposus upon axial loading of the intervertebral disc: An experimental in situ study

Cited by 7 publications

References 31 publications

One strike loading organ culture model to investigate the post-traumatic disc degenerative condition

One strike loading organ culture model to investigate the post-traumatic disc degenerative condition

Intervertebral disc injury is the mainspring for the postoperative increase in Cobb Angle after thoracolumbar burst fracture

Neoepitope fragments as biomarkers for different phenotypes of intervertebral disc degeneration

Contact Info

Product

Resources

About