Stiffening of connective tissue in elderly diabetic patients: relevance to diabetic nephropathy and oxidative stress

Abstract

Limited joint mobility seen in diabetes mellitus is thought to be the result of stiffening of periarticular connective tissue, which is presumably derived from increased cross-linking of collagen related to advanced glycation end products. In this study the extent of the stiffening of connective tissue was measured by the passive extension angle of the metacarpophalangeal joints in 205 elderly diabetic patients. Association with diabetic nephropathy, with which advanced glycation end products have recently been demonstrated to increase, and metabolic abnormalities were also considered. The angle of the metacarpophalangeal joints was significantly correlated with age (r = -0.24, p < 0.01), and was significantly smaller in men than in women (p < 0.01). The angle demonstrated a decrease in association with diabetic retinopathy and nephropathy, and only the association with nephropathy was significant (p < 0.05). The angle was weakly, but significantly, correlated with serum thiobarbituric acid reactants as a measure of lipid peroxides (r = -0.15, p < 0.05), triglyceride (r = -0.20, p < 0.01) and HDL cholesterol (r = 0.19, p < 0.01), but not with blood glucose (r = 0.02), HbA1c (r = 0.06) or duration of diabetes (r = -0.05). In addition, the angle in 14 non-diabetic patients on haemodialysis was significantly (p < 0.05) smaller than that in age- and sex-matched normal subjects. Thus, it was indicated that the stiffening of connective tissue was associated with diabetic nephropathy, serum lipid peroxide and dyslipidaemia. Stiffening of connective tissue seems to be more affected by oxidative stress than non-enzymatic glycation per se.(ABSTRACT TRUNCATED AT 250 WORDS)

Associations between hyperglycaemia and somatic transversion mutations in mitochondrial DNA of people with diabetes mellitus

“…In addition, no correlation was found between urinary 8-OHdG and the transversion mutational burden. However, oxidative stress is reported to be increased in people with diabetes mellitus or hyperglycaemia [3,4,5,6,7,8,9,10,11,12,13,14,15], suggesting that the somatic transversion mutational burden of mDNA is associated with oxidative stress derived from hyperglycaemia, which is not reflected by urinary 8-OHdG.…”

Relationship between limited joint mobility syndrome and duration, metabolic control, complications of diabetes as well as effects of the syndrome on quality of life

“…The rate of nephropathy in patients with LJM was reported as 40-90.9% in the literature and the relation with both micro and macroalbuminuria was shown [10]. In the study of Aoki et al [20] it was reported that nephropathy stage increased with the increasing severity of LJM.…”

Combining nano-physical and computational investigations to understand the nature of &ldquo;aging&rdquo; in dermal collagen

“…Its more reactive open chain form only composes 0.002% of glucose molecules in vivo; therefore, significant collagen cross-linking requires a prolonged exposure to glucose. 23 A number of other AGE cross-links can also form, such as pentosidine, 24 DOGDIC (deoxyglucosone lysine-arginine cross-link), MOLD (methylglyoxal lysine dimer) and GOLD (glyoxal lysine dimer), 17,21 among others ( Figure 2). Deoxyglucosone and glyoxal are formed due to fragmentation of the Amadori product (an intermediate precursor product to an AGE in the Maillard reaction) and are highly reactive, although they are found in very small concentrations in vivo.…”