Stiffening Effect of Cholesterol on Disordered Lipid Phases: A Combined Neutron Spin Echo + Dynamic Light Scattering Analysis of the Bending Elasticity of Large Unilamellar Vesicles

Arriaga, Laura R.; López‐Montero, Iván; Monroy, Francisco; Orts-Gil, Guillermo; Faragó, B.; Hellweg, Thomas

doi:10.1016/j.bpj.2009.01.045

Cited by 106 publications

(116 citation statements)

References 64 publications

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“…4 A and are inversely proportional to the intercept of the fit with the vertical axis, as described in more detail in Materials and Methods. From this analysis we obtained a bending modulus estimate of the bare bilayer system of (1.5 5 0.13) Â 10 À20 J, close to the values previously reported for simulations of pure DPPC lipid bilayers (4 Â 10 À20 J) (50) and to experimental data for POPC bilayers ranging between 6.7 and 16 Â 10 À20 J (64)(65)(66)(67)(68). The values obtained in our simulations are slightly lower than previously reported, which might be due to the temperature: our simulations are performed at physiological temperature (310 K, 37 C) whereas previously reported experimental data were obtained at lower temperatures.…”

Section: Effects Of Povpc and Pgpc On The Bending Modulus Of The Bilasupporting

confidence: 85%

Molecular-Scale Biophysical Modulation of an Endothelial Membrane by Oxidized Phospholipids

Ayee

LeMaster

Shentu

et al. 2017

Biophysical Journal

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The influence of two bioactive oxidized phospholipids on model bilayer properties, membrane packing, and endothelial cell biomechanics was investigated computationally and experimentally. The truncated tail phospholipids, 1-palmitoyl-2-(5-oxovaleroyl)-sn-glycero-3-phosphocholine (POVPC) and 1-palmitoyl-2-glutaroyl-sn-glycero-3-phosphocholine (PGPC), are two major oxidation products of the unsaturated phospholipid 1-palmitoyl-2-arachidonoyl-sn-glycero-phosphocholine. A combination of coarse-grained molecular dynamics simulations, Laurdan multiphoton imaging, and atomic force microscopy microindentation experiments was used to determine the impact of POVPC and PGPC on the structure of a multicomponent phospholipid bilayer and to assess the consequences of their incorporation on membrane packing and endothelial cell stiffness. Molecular simulations predicted differential bilayer perturbation effects of the two oxidized phospholipids based on the chemical identities of their truncated tails, including decreased bilayer packing, decreased bilayer bending modulus, and increased water penetration. Disruption of lipid order was consistent with Laurdan imaging results indicating that POVPC and PGPC decrease the lipid packing of both ordered and disordered membrane domains. Computational predictions of a larger membrane perturbation effect by PGPC correspond to greater stiffness of PGPC-treated endothelial cells observed by measuring cellular elastic moduli using atomic force microscopy. Our results suggest that disruptions in membrane structure by oxidized phospholipids play a role in the regulation of overall endothelial cell stiffness.

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Section: Effects Of Povpc and Pgpc On The Bending Modulus Of The Bilasupporting

confidence: 85%

Molecular-Scale Biophysical Modulation of an Endothelial Membrane by Oxidized Phospholipids

Ayee

LeMaster

Shentu

et al. 2017

Biophysical Journal

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“…While the phase behavior is largely independent of the type of phospholipid, PFOA itself is found to partition more readily into lipid bilayers in their fluid phase [13]. Several studies have taken advantage of a combination of dynamic light scattering (DLS) and long-wavelength NSE for the investigation of the local bilayer undulation dynamics in unilamellar lipid vesicles (ULVs) [16][17][18][19][20][21]. Here, we cover a window of more than 200 ns.…”

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confidence: 99%

Perfluorooctanoic acid rigidifies a model lipid membrane

Brüning

Faragó

2014

Phys. Rev. E

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We report a combined dynamic light scattering and neutron spin-echo (NSE) study on vesicles composed of the phospholipid 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine under the influence of varying amounts of perfluorooctanoic acid. We study local lipid bilayer undulations using NSE on time scales up to 200 ns. Similar to the effect evoked by cholesterol, we attribute the observed lipid bilayer stiffening to a condensing effect of the perfluorinated compound on the membrane. Perfluorinated compounds (PFCs) are fully fluorinated fatty acid analogs commonly used in a wide range of applications, such as the production of fire-extinguishing foams, anticorrosion agents, lubricants, or cosmetics [1,2]. As a consequence of their chemical stability, these compounds exhibit an environmental stability and are transmitted into the mammal food chain [3]. Due to their tendency to bioaccumulate, the compounds affect properties of cell membranes, causing developmental and reproductive disorders [2,4]. It is of particular interest to understand the effect of perfluorinated compounds not only on cellular membranes [5,6], but also on their biomimetic counterparts [7]. In mammal organisms, vesicular membranes often serve as natural carriers. It is assumed that functional properties of a membrane depend likewise on its composition-dependent structure and dynamics [8,9]. In order to gain insight into membrane function, specific material properties, such as, e.g., the bilayer bending rigidity κ can be aimfully influenced. Several studies have addressed the insertion of perfluorinated compounds into binary model membranes. Oriented mono-and bilayers were investigated by Matyszewska et al. using methods including surface pressure and potential measurements, infrared spectroscopy (IR), nuclear magnetic resonance (NMR) techniques, and molecular dynamics (MD) simulations [4,10,11]. While the phospholipid head group tilt against the bilayer normal decreases with rising amounts of inserted perfluorooctanoic acid (PFOA), the lipid acyl chain order increases. Lateral molecule diffusion in the membrane plane changes nonuniformly as the ratio of the components in the binary mixture is varied [10]. Lehmler et al. study liposomes containing binary mixtures of varying phospholipids and perfluorinated surfactants [12][13][14][15]. They use fluorescence spectroscopy and differential scanning calorimetry measurements to study the partitioning of surfactants into the phospholipid bilayer and find that it is independent of the lipid acyl chain length. While the phase behavior is largely independent of the type of phospholipid, PFOA itself is found to partition more readily into lipid bilayers in their fluid phase [13]. Several studies have taken advantage of a combination of dynamic light scattering (DLS) and long-wavelength NSE for the investigation of the local bilayer undulation dynamics in unilamellar lipid vesicles (ULVs) [16][17][18][19][20][21]. Here, we cover a window of more than 200 ns. The membrane dynamics was investigated by NSE using w...

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“…Within the investigated temperature range no significant change of the bending elasticity constants obtained from static and dynamic Fig. 6 Undulation relaxation rates G u as a function of q 3 for four sample temperatures in the range 283 K-302 K. From linear fits to the data (solid lines) the bending elasticity constant k was calculated using eqn (10). measurements was found.…”

Section: Determination Of J By Combing Nse and Dlsmentioning

confidence: 99%

“…[7][8][9][10][11] In some of these cases the slow mode is related to center of mass diffusion, but also bicontinuous systems exhibit slow, diffusional, collective modes.…”

Section: Introductionmentioning

confidence: 99%

“…In this work, a combination of NSE and dynamic light scattering (DLS) measurements is used to identify both types of contributions to the NSE signal by including additional measurements of the normalized intensity time autocorrelation function g 2 (t) in the q-range q{q max , where q max represents the maximum of the static structure factor S(q). 10,28,29 The results from the quasielastic scattering experiments will be compared to data derived from SAXS measurements.…”

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confidence: 99%

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Temperature dependence of the surfactant film bending elasticity in a bicontinuous sugar surfactant based microemulsion: a quasielastic scattering study

Wellert

Karg

Holderer

et al. 2011

Phys. Chem. Chem. Phys.

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Currently, the design of microemulsions is focussed on the formulation of environmentally compatible systems formed by non-harmful amphiphiles and oils. The use of sugar-based surfactants allows the design of microemulsions where, instead of the temperature, the addition of short-or medium-chain alcohols tunes the curvature of the amphiphilic interface. In this work, the resulting temperature stability of a sugar surfactant and rapeseed methyl ester based bicontinuous microemulsion is exploited to study the influence of temperature variations on the bending elastic constant k. Quasi-elastic scattering of light and neutrons is used to separate long-range collective motions and local thermally excited undulations of the interface. k in units of kT is found to be independent of temperature over a wide range.

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Stiffening Effect of Cholesterol on Disordered Lipid Phases: A Combined Neutron Spin Echo + Dynamic Light Scattering Analysis of the Bending Elasticity of Large Unilamellar Vesicles

Cited by 106 publications

References 64 publications

Molecular-Scale Biophysical Modulation of an Endothelial Membrane by Oxidized Phospholipids

Molecular-Scale Biophysical Modulation of an Endothelial Membrane by Oxidized Phospholipids

Perfluorooctanoic acid rigidifies a model lipid membrane

Temperature dependence of the surfactant film bending elasticity in a bicontinuous sugar surfactant based microemulsion: a quasielastic scattering study

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