STI with Mycoplasma genitalium—more common than Chlamydia trachomatis in patients attending youth clinics in Sweden

Nolskog, Peter; Backhaus, Erik; Nasic, Salmir; Enroth, Helena

doi:10.1007/s10096-018-3395-3

Cited by 16 publications

(12 citation statements)

References 18 publications

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“…M. genitalium is also a suspected cause of reactive arthritis and proctitis [13]. Characteristics contributing to increased risk of M. genitalium infections include living in low-and-middle-income localities [14][15][16][17], being symptomatic [18], experiencing fertility problems for both men [19,20] and women [21], abnormal pregnancy status [22], and being members of vulnerable populations, including men-who-have-sex-with men (MSM) [23,24], female sex workers (FSW) [25], and people living with HIV [26]. These considerations have spurred clinicians and public health agencies to call for global coordination of M. genitalium guidelines and treatment to help mitigate AMR-related problems [1][2][3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Macrolide and fluoroquinolone associated mutations in Mycoplasma genitalium in a retrospective study of male and female patients seeking care at an STI Clinic in Guangzhou, China, 2016-2018.

Tso

et al. 2020

Preprint

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BackgroundAntimicrobial resistance in M. genitalium is a growing clinical problem. We investigated the mutations associated with macrolide and fluoroquinolone resistance, two commonly used medical regimens for treatment in China. Our aim is to analyze the prevalence and diversity of mutations among M. genitalium-positive clinical specimens in Guangzhou, south China.MethodsA total of 154 stored M. genitalium positive specimens from men and women attending a STI clinic were tested for macrolide and fluoroquinolone mutations. M. genitalium was detected via TaqMan MGB real-time PCR. Mutations associated with macrolide resistance were detected using primers targeting region V of the 23S rRNA gene. Fluoroquinolone resistant mutations were screened via primers targeting topoisomerase IV (parC) and DNA gyrase (gyrA).Results98.7% (152/154), 95.5% (147/154) and 90.3% (139/154) of M. genitalium positive samples produced sufficient amplicon for detecting resistance mutations in 23S rRNA, gyrA and parC genes, respectively. 66.4% (101/152), 0.7% (1/147) and 77.7% (108/139) samples manifested mutations in 23S rRNA, gyrA and parC genes, respectively. A2072G (59/101, 58.4%) and S83I (79/108, 73.1%) were highly predominating in 23S rRNA and parC genes, respectively. Two samples had amino acid substitutions in gyrA (M95I and A96T, respectively). Two samples had two amino acid substitutions in parC (S83I + D87Y). 48.6% (67/138) of samples harbored both macrolide and fluoroquinolone resistance-associated mutations. The most common combination of mutations was A2072G (23S rRNA) and S83I (parC) (40/67, 59.7%). One sample had three amino acid changes in 23S rRNA, gyrA and parC genes (A2072G + A96T + S83I).ConclusionsThe high antimicrobial resistance rate of M. genitalium in Guangzhou is a very worrying problem and suggests that antimicrobial resistance testing and the development of new antibiotic regimens are crucially needed.

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Section: Introductionmentioning

confidence: 99%

Macrolide and fluoroquinolone associated mutations in Mycoplasma genitalium in a retrospective study of male and female patients seeking care at an STI Clinic in Guangzhou, China, 2016-2018.

Tso

et al. 2020

Preprint

View full text Add to dashboard Cite

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“…In our study, symptomatic patients were more likely to have MG infection compared to asymptomatic individuals(12.5% Vs 5.7% ,p = 0.004). This was also reported in men but not in women for patients attending a young person clinic in Sweden [41]. However,some studies had the opposite conclusions, such as no statistical association between clinical symptoms and MG infection in men from an STI clinic in Guangxi Province in China [26], and no association between MG infection and microscopically defined urethritis or cervicitis in Greece [29].…”

Section: Discussionmentioning

confidence: 84%

Prevalence and Correlates of Mycoplasma genitalium Infections among Patients Attending a Sexually Transmitted Infection Clinic in Guangdong, China a Cross-sectional Study

Zhang

Zhao

et al. 2020

Preprint

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Background Mycoplasma genitalium (MG) is known to cause urogenital tract infections and is associated with reproductive morbidity. Although MG has been reported across many regions and population groups, it is not yet routinely tested for in China. Our study contributes to current research by reporting the prevalence and correlates of MG for patients attending a sexually transmitted infection (STI) clinic in Guangdong from Jan 2017-May 2018.Methods Urethral (489 men) and endo-cervical (189 women) samples, blood samples, and patient histories (via questionnaires) were collected. Doctors clinically diagnosed anogenital warts (GW) during the examination (n=678). Presence of MG was tested using an in-house polymerase chain reaction. We also tested all participants for herpes simplex virus-2 (HSV-2), Neisseria gonorrhoeae (NG), Chlamydia trachomatis (CT), syphilis and HIV. Univariate and multivariable logistic regressions were used to evaluate factors associated with MG.Results MG was detected among 7.2% (n=49) of patients (7.4% men, 6.9% women). Of 629 heterosexual patients, the MG was detected in 7.5% (47/629), and of 49 MSM, MG was detected in 4.1% (2/49). Among MG-infected patients, 10.2% were co-infected with CT, 6.1% with NG, 8.2% with HSV-2, 4.1% with syphilis and 22.4% with GW. Among 152 symptomatic patients, the prevalence of MG was 12.5% (n=19, of whom 17 were men). Being symptomatic was statistically significant for MG infection [OR=2.2(1.3~3.8)].Conclusion MG is a relatively common infection among individuals attending an STI clinic in Guangdong province. To make MG testing available and routinely screen symptomatic patients with urinary and /or cervical discharge in STI clinics in China is essential.

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“…In our study, symptomatic patients were more likely to have MG infection than asymptomatic individuals (14.2% vs. 5.6%, P=0.001). This pattern was also reported for men but not women among patients attending a young person's clinic in Sweden [31]. However, some studies arrived at the opposite conclusions, i.e., nding no statistical association between clinical symptoms and MG infection in men attending an STI clinic in Guangxi Province, China [22], and no association between MG infection and microscopically de ned urethritis or cervicitis in patients in Greece [25].…”

Section: Correlates Of Mg Infectionsmentioning

confidence: 82%

Prevalence and Correlates of Mycoplasma genitalium Infections among Patients Attending a Sexually Transmitted Infection Clinic in Guangdong, China a Cross-sectional Study

Zhang

Zhao

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Mycoplasma genitalium (MG) causes urogenital tract infections and is associated with reproductive morbidity. Although MG has been reported across many regions and population groups, it is not yet routinely tested for in China. Our study contributes to current research by reporting the prevalence and correlates of MG infection in patients attending a sexually transmitted infection (STI) clinic in Guangdong from Jan 2017-May 2018.Methods: Urethral (from 489 men) and endo-cervical (from 189 women) samples, blood samples, and patient histories (via questionnaires) were collected. Doctors clinically diagnosed anogenital warts (GW) during the examination (n=678). The presence of MG was evaluated using an in-house via polymerase chain reaction protocol. We also tested all participants for herpes simplex virus-2 (HSV-2), Neisseria gonorrhoeae (NG), Chlamydia trachomatis (CT), syphilis and HIV. Univariate and multivariate logistic regression were used to evaluate factors associated with MG.Results: MG was detected in 7.2% (49/678) of the patients (men, 7.4%; women, 6.9%). The MG positivity rate was 14.2% among symptomatic patients, and 5.6% for asymptomatic patients , respectively. Only 36.7% (18/49) Mg positive patients were symptomatic. Among the MG-infected patients, 10.2% were co-infected with CT, 6.1% with NG, 8.2% with HSV-2, 4.1% with syphilis and 22.4% with GW. Presentation with clinical symptoms was significantly associated with MG infection [OR=2.52 (2.03-3.13)]. In our analysis, MG was not associated with other STIs.Conclusions: MG is a relatively common infection among individuals attending an STI clinic in Guangdong Province. Routine testing of symptomatic patients may be necessary, and more epidemiological studies are needed to provide evidence for future testing guidelines,

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STI with Mycoplasma genitalium—more common than Chlamydia trachomatis in patients attending youth clinics in Sweden

Cited by 16 publications

References 18 publications

Macrolide and fluoroquinolone associated mutations in Mycoplasma genitalium in a retrospective study of male and female patients seeking care at an STI Clinic in Guangzhou, China, 2016-2018.

Macrolide and fluoroquinolone associated mutations in Mycoplasma genitalium in a retrospective study of male and female patients seeking care at an STI Clinic in Guangzhou, China, 2016-2018.

Prevalence and Correlates of Mycoplasma genitalium Infections among Patients Attending a Sexually Transmitted Infection Clinic in Guangdong, China a Cross-sectional Study

Prevalence and Correlates of Mycoplasma genitalium Infections among Patients Attending a Sexually Transmitted Infection Clinic in Guangdong, China a Cross-sectional Study

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