Sterols as dietary markers for Drosophila melanogaster

Knittelfelder, Oskar; Prince, Elodie; Sales, Susanne; Fritzsche, Eric; Wöhner, Thomas; Brankatschk, Marko; Shevchenko, Andrej

doi:10.1016/j.bbalip.2020.158683

Cited by 27 publications

(69 citation statements)

References 25 publications

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“…Because D. melanogaster males continuously produce sperm and because we show diet‐based differences in sperm production, it is likely that seasonal changes in food preference identified by Knittelfelder et al () will translate into seasonal changes in sperm production. However, even more interesting, this will not be a simple dynamic following seasonal temperature changes because the lipid uptake did not follow a stoichiometric pattern: PF sterol taken up with diet replaced YF sterols (ergosterol) in the membranes of the reproductive tract of YF males, but YF sterols did not replace existing PF sterols (sitosterol and campesterol) (Knittelfelder et al, ). This asymmetry is unlikely caused by biochemical constraints because the YF/PF sterol replacement was symmetrical in female reproductive tracts (Knittelfelder et al, ).…”

Section: Discussionmentioning

confidence: 77%

“…However, even more interesting, this will not be a simple dynamic following seasonal temperature changes because the lipid uptake did not follow a stoichiometric pattern: PF sterol taken up with diet replaced YF sterols (ergosterol) in the membranes of the reproductive tract of YF males, but YF sterols did not replace existing PF sterols (sitosterol and campesterol) (Knittelfelder et al, ). This asymmetry is unlikely caused by biochemical constraints because the YF/PF sterol replacement was symmetrical in female reproductive tracts (Knittelfelder et al, ). We may, therefore, speculate about an adaptive benefit to males of preventing the replacement of their PF sterols.…”

Section: Discussionmentioning

confidence: 99%

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Dietary polyunsaturated fatty acids affect volume and metabolism of Drosophila melanogaster sperm

Guo

Reinhardt

2020

J of Evolutionary Biology

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Dietary fatty acids can accumulate in sperm and affect their function in vertebrates. As Drosophila melanogaster shares several pathways of lipid metabolism and shows similar lipid‐dependent phenotypes but lacks some hormones that in vertebrates regulate lipid metabolism, there is currently no clear prediction as to how dietary fatty acids affect the sperm of D. melanogaster. We manipulated the amount and identity of dietary polyunsaturated fatty acids (PUFA) in the food of D. melanogaster males (a treatment known to affect membrane fluidity) and measured changes in sperm parameters. We found that (a) males reared on food containing PUFA‐rich, plant‐derived lipids showed a slower increase in sperm volume over male age compared to males reared on yeast‐derived lipid food which is richer in saturated fatty acids. (b) The resistance of sperm membrane integrity to osmotic stress was not altered by dietary lipid treatment, but (c) food containing yeast‐derived lipids induced a 46% higher in situ rate of production of reactive oxygen species in sperm cells. These findings show that dietary lipids have similar effects on sperm parameters in Drosophila as in vertebrates, affect some, but not all, sperm parameters and modulate male reproductive ageing. In concert with recent findings of sex‐specific seasonal variation of diet choice in the wild, our results suggest a substantial dietary impact on the dynamics of male reproduction in the wild.

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Section: Discussionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Dietary polyunsaturated fatty acids affect volume and metabolism of Drosophila melanogaster sperm

Guo

Reinhardt

2020

J of Evolutionary Biology

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“…To evaluate the contribution of individual dIlps, we have tracked the development of different ΔdIlp mutants at comfortable temperatures (20°C) or upon heat stress (28°C). At 20°C, all tested mutants, including ΔdIlp 2 , ΔdIlp 3 , ΔdIlp 5 ,ΔdIlp [2][3] or ΔdIlp [2][3]5 , survive (at 20°C, nlarva=132, control (Cntr)=132 ±0% and nlarva= 140, ΔdIlp [2][3]5 =72.1 ±4.3%, p=****); although the developmental rate of ΔdIlp [2][3]5 is much slower and produces small larvae and pupae with respect to controls (Figure 1 and Supplementary Figure S1 and (37,38).…”

Section: Yeast Food Is Not Sufficient To Replace Ipc Activity At Highmentioning

confidence: 95%

“…Thriving in new ecological systems, Drosophila learned to utilize local food. Such resources vary in their composition in response to environmental temperature changes (1)(2)(3). Wild fruit flies feed on rotting fruits i.e.…”

Section: Introductionmentioning

confidence: 99%

Diet and heat - one neuronal subset two responses

Prince¹,

Kretzschmar²,

Trautenberg³

et al. 2020

Preprint

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The Insulin signal cascade is one of the best studied metabolic circuits, and shows a remarkable high molecular and functional conservation across the animal kingdom. Insulin-producing cells respond directly to nutritional cues in circulation and receive modulatory input from connected neuronal networks. Neuronal control is rapid and integrates a wide range of variables including dietary change or environmental temperature. However, despite various detailed studies that demonstrated the potential of neuronal regulation the physiological relevance of this circuit remains elusive.In Drosophila, Insulin-like peptide 7 (dIlp7)-producing neurons are wired with Insulin-producing cells. We found a dual role for this neuronal subset: a.) activated dilp7-producing neurons are required to facilitate development at high temperatures, and if confronted with calorie-rich food that represses neuronal activity b.) their product, dIlp7, regulates Insulin signalling levels. Our work shows that Insulin-producing cells not simply integrate signals from circulating nutritional cues and neuronal inputs, but switch to neuronal control in response to dietary composition.

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“…For sterol and lipidome measurements, samples were diluted 1:1 with spray solution. Mass spectrometric analysis was performed as described (Knittelfelder et al, 2020).…”

Section: Lipid Extraction and Quantification By Shotgun Mass Spectrommentioning

confidence: 99%

A non-canonical lipid droplet metabolism regulates the conversion of alpha-Synuclein to proteolytic resistant forms in neurons of aDrosophilamodel of Parkinson disease

Girard

Jollivet

Knittelfelder

et al. 2020

Preprint

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Parkinson’s disease is a neurodegenerative disorder characterized by accumulation of alpha-synuclein (αSyn) aggregates and by abnormalities in lipid storage. To investigate the potential pathophysiological consequences of interactions between αSyn and proteins that regulate the homeostasis of intracellular lipid droplets (LDs), we employed a transgenic Drosophila model of PD in which human αSyn is specifically expressed in photoreceptor neurons. We found that overexpression of the LD-coating proteins perilipin 1 and 2 (dPlin1/2) markedly increased LD accumulation in the neurons. Perilipins also co-localized with αSyn at the LD surface in both Drosophila photoreceptor neurons (dPlin2) and human neuroblastoma cells (PLIN3). Co-expression of αSyn and dPlin2 in photoreceptor neurons synergistically amplified LD content through a mechanism involving LD stabilization, independently of Brummer-mediated lipolysis or de novo synthesis of triacylglycerols. Accumulation of LDs also increased the resistance of αSyn to proteolytic digestion, a phenomenon associated with αSyn aggregation in human neurons. Our results suggest that binding of αSyn to PLIN-coated LDs stabilizes the LD structure and may contribute to the pathogenic misfolding and aggregation of αSyn in neurons.

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Sterols as dietary markers for Drosophila melanogaster

Cited by 27 publications

References 25 publications

Dietary polyunsaturated fatty acids affect volume and metabolism of Drosophila melanogaster sperm

Dietary polyunsaturated fatty acids affect volume and metabolism of Drosophila melanogaster sperm

Diet and heat - one neuronal subset two responses

A non-canonical lipid droplet metabolism regulates the conversion of alpha-Synuclein to proteolytic resistant forms in neurons of aDrosophilamodel of Parkinson disease

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