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The product'ion of sterols, more particularly ergosterol the precursor of vitamin Dfrom fungi was the aim of many investigators since the isolation of ergosterol from ergot by TANRET in 1889. Different species of mould fungi mostly aspergilli and penicillia had been studied for sterol production (PRUESS et al. 1931, 1932, TAPPI 1948 and EL-REFAI 1964.Also yeasts had been received attention as source of sterols. BrLLs et al. (1930) surveyed strains of thirteen species in five genera including Endomyces, Nadsonia, Mycoderma, Saccharomyces and Zygosacch,aromyces. The ergosterol contents were approximately 0.2 to 0.3% in all cultures except those of the genus Saceharomyces which varied greatly in ergosterol contents. Different st'rains of one species of Sacch. cerevisiae ranged from 0.2 to 1. 4%. PROSKURYAKOV et al. (1938) also found that the different strains of one yeast species cult,ivated under identical conditions showed wide variations in ergosterol. Contents ranged from 0.31 to 2.5%. DULANEY et aZ. (1954) and GAL'TSOVA et al. (1959) stated that ergosterol production was extremely low in all genera except in the genus Saccharomyces. According to APPLETON and his co-workers (1955) members of tJhe Endomycetaceae yield rather more than 0.5% of sterols, while the Cryptococcaceae produce less with the exception of Rhodotorula gracilis. CONZALEZ and CAMPILLO( 1961) studied the ergosterol contents of 39strains of soil yeasts belonging to the following genera : Xaccha,romyces, Debaryomyces, Hansenula, Pichia, Rhodotorula and l'orulopsis. Members of the genus Saccharomyces had the highest sterol contents compared wibh other yeasts. I n this paper, the ability of different locally isolated yeasts for sterol product'ion was investigated. The most promising species were furtheron surveyed for ergosterol production. Material and methodsThe different yeast organisms used in this work were isolated from different local habitats. Collections were made from leaves of various herbs, sap and slime flux from trees, ripe fruits, infected biere and the superfacial layer of Egyptian soil. Samples of each of t.he collected materials were inoculated on t'he growth medium: glucose, 10.0; peptone, 2.0;KH,PO,, 7.0; and MgSO, . 7H,O, 0.5 g/1 distilled water. After seven days a considerable growth was achieved. Different yeash were then isolated by diluting and streaking of a sample of t.he growing organisms on plates of Hacto wart.-agar supplemented with 0.25 to 0.35 g/I of sodium propionat.e to inhibit the growth of the contaminat.ing moulds.Purification from bacteria was carried out by inoculating the isolated yeasts on wort-agar plates acidified with one drop of 25-5076 lact,ic acid. One of the developed yeast colonies was then further transferred to another wort-agar plate to which aureomycine (25 pg/ml) was added. The purity of the yeast strains were cheked by direct microscopic examiriat'ion and by cultivation on different media favouring t,he growth of bacteria ( T A H~ and EL-REFAI 1963). 26 Zeitschrift f. All& Mikrobiologie...
The product'ion of sterols, more particularly ergosterol the precursor of vitamin Dfrom fungi was the aim of many investigators since the isolation of ergosterol from ergot by TANRET in 1889. Different species of mould fungi mostly aspergilli and penicillia had been studied for sterol production (PRUESS et al. 1931, 1932, TAPPI 1948 and EL-REFAI 1964.Also yeasts had been received attention as source of sterols. BrLLs et al. (1930) surveyed strains of thirteen species in five genera including Endomyces, Nadsonia, Mycoderma, Saccharomyces and Zygosacch,aromyces. The ergosterol contents were approximately 0.2 to 0.3% in all cultures except those of the genus Saceharomyces which varied greatly in ergosterol contents. Different st'rains of one species of Sacch. cerevisiae ranged from 0.2 to 1. 4%. PROSKURYAKOV et al. (1938) also found that the different strains of one yeast species cult,ivated under identical conditions showed wide variations in ergosterol. Contents ranged from 0.31 to 2.5%. DULANEY et aZ. (1954) and GAL'TSOVA et al. (1959) stated that ergosterol production was extremely low in all genera except in the genus Saccharomyces. According to APPLETON and his co-workers (1955) members of tJhe Endomycetaceae yield rather more than 0.5% of sterols, while the Cryptococcaceae produce less with the exception of Rhodotorula gracilis. CONZALEZ and CAMPILLO( 1961) studied the ergosterol contents of 39strains of soil yeasts belonging to the following genera : Xaccha,romyces, Debaryomyces, Hansenula, Pichia, Rhodotorula and l'orulopsis. Members of the genus Saccharomyces had the highest sterol contents compared wibh other yeasts. I n this paper, the ability of different locally isolated yeasts for sterol product'ion was investigated. The most promising species were furtheron surveyed for ergosterol production. Material and methodsThe different yeast organisms used in this work were isolated from different local habitats. Collections were made from leaves of various herbs, sap and slime flux from trees, ripe fruits, infected biere and the superfacial layer of Egyptian soil. Samples of each of t.he collected materials were inoculated on t'he growth medium: glucose, 10.0; peptone, 2.0;KH,PO,, 7.0; and MgSO, . 7H,O, 0.5 g/1 distilled water. After seven days a considerable growth was achieved. Different yeash were then isolated by diluting and streaking of a sample of t.he growing organisms on plates of Hacto wart.-agar supplemented with 0.25 to 0.35 g/I of sodium propionat.e to inhibit the growth of the contaminat.ing moulds.Purification from bacteria was carried out by inoculating the isolated yeasts on wort-agar plates acidified with one drop of 25-5076 lact,ic acid. One of the developed yeast colonies was then further transferred to another wort-agar plate to which aureomycine (25 pg/ml) was added. The purity of the yeast strains were cheked by direct microscopic examiriat'ion and by cultivation on different media favouring t,he growth of bacteria ( T A H~ and EL-REFAI 1963). 26 Zeitschrift f. All& Mikrobiologie...
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