Sterol biosynthesis in heterotrophic plant parasites

Rohmer, Michel; Ourisson, Guy; Benveniste, Pierre; Bimpson, T.

doi:10.1016/0031-9422(75)83022-6

Cited by 22 publications

(10 citation statements)

References 7 publications

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“…The steroid cycloartenol is present in small amounts in many plants [23]. The cycloartenol pathway is known to be present in parasitic plants (i.e., Cuscuta and Orobanche ), and large amounts of cycloartenol and its derivatives are present in Cuscuta [19,24]. Those studies analyzed only the parasitic plant attachment to host plants, but did not compare parasitic plants before and after parasitization.…”

Section: Discussionmentioning

confidence: 99%

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Analysis of Metabolites in Stem Parasitic Plant Interactions: Interaction of Cuscuta–Momordica versus Cassytha–Ipomoea

Furuhashi

Nakamura

Iwase

2016

Plants

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Cuscuta and Cassytha are two well-known stem parasitic plant genera with reduced leaves and roots, inducing haustoria in their stems. Their similar appearance in the field has been recognized, but few comparative studies on their respective plant interactions are available. To compare their interactions, we conducted a metabolite analysis of both the Cassytha–Ipomoea and the Cuscuta–Momordica interaction. We investigated the energy charge of the metabolites by UFLC (ultra-high performance liquid chromatography), and conducted GC-MS (gas chromatography-mass spectrometry) analysis for polar metabolites (e.g., saccharides, polyols) and steroids. The energy charge after parasitization changed considerably in Cassytha but not in Cusucta. Cuscuta changed its steroid pattern during the plant interaction, whereas Cassytha did not. In the polar metabolite analysis, the laminaribiose increase after parasitization was conspicuous in Cuscuta, but not in Cassytha. This metabolite profile difference points to different lifestyles and parasitic strategies.

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Section: Discussionmentioning

confidence: 99%

“…Steroids can be upstream of some plant hormones (i.e., brassinolide) and also play a role in the immune system [19,20]. As plant interactions provoke hormonal changes and pathogenic responses, it is interesting to conduct steroid profiling.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Metabolites in Stem Parasitic Plant Interactions: Interaction of Cuscuta–Momordica versus Cassytha–Ipomoea

Furuhashi

Nakamura

Iwase

2016

Plants

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“…The acetates of the three major sterols were separated one from another by t.l.c. (three migrations) on silica gel impregnated with AgNO3 (Rohmer et al, 1975), with cyclohexane/toluene (7:3, v/v), and gave the acetates of compound (VIII) ( Fig. 1) and other minor sterols (RF 0.54), a mixture of acetates of minor sterols (RF 0.32) and a mixture of the acetates ofcompounds (V) and (VI) ( Fig.…”

Section: Culture Of Acanthamoeba Polyphagamentioning

confidence: 99%

Sterol biosynthesis de nova via cycloartenol by the soil amoeba Acanthamoeba polyphaga

Raederstorff¹,

Rohmer²

1985

Biochemical Journal

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The soil amoeba Acanthamoeba polyphaga is capable of synthesizing its sterols de novo from acetate. The major sterols are ergosterol and poriferasta-5,7,22-trienol. Furthermore C28 and C29 sterols of still unknown structure with an aromatic B-ring are also synthesized by the amoeba. The first cyclic sterol precursor is cycloartenol, which is the sterol precursor in all photosynthetic phyla. No trace of lanosterol, which is the sterol precursor in animals and fungi, could be detected. These results show that at least some of the biochemical processes of Acanthamoeba polyphaga might be phylogenetically related to those of unicellular algae. Addition of exogenous sterols to the culture medium does not influence the sterol biosynthesis and the sterol composition of the cells.

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“…When I started my PhD thesis in 1970, I was working with Guy Ourisson and Pierre Benveniste on sterol 1 (Fig. 1) biosynthesis in maize [2], in the non-photosynthetic euglenoid Astasia longa [3] and in parasitic non-phototrophic higher plants such as broomrape and dodder [4]. At that time, George's work in the field of sterol isolation, identification and biosynthesis was quite familiar to me.…”

Section: Introductionmentioning

confidence: 99%

From Molecular Fossils of Bacterial Hopanoids to the Formation of Isoprene Units: Discovery and Elucidation of the Methylerythritol Phosphate Pathway

Rohmer

2008

Lipids

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Investigations on the biosynthesis of bacterial triterpenoids of the hopane series led to the unexpected discovery of an alternative mevalonate independent pathway for the formation of isoprene units. Methylerythritol phosphate, already presenting the C5 branched isoprene skeleton, is the key intermediate. This pathway was independently characterized in ginkgo embryos for the formation of diterpenoids. It is present in most bacteria and in the plastids of all organisms belonging to phototrophic phyla. The key steps of the discovery and elucidation of this metabolic route are presented in this review.

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Sterol biosynthesis in heterotrophic plant parasites

Cited by 22 publications

References 7 publications

Analysis of Metabolites in Stem Parasitic Plant Interactions: Interaction of Cuscuta–Momordica versus Cassytha–Ipomoea

Analysis of Metabolites in Stem Parasitic Plant Interactions: Interaction of Cuscuta–Momordica versus Cassytha–Ipomoea

Sterol biosynthesis de nova via cycloartenol by the soil amoeba Acanthamoeba polyphaga

From Molecular Fossils of Bacterial Hopanoids to the Formation of Isoprene Units: Discovery and Elucidation of the Methylerythritol Phosphate Pathway

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