Steroidumwandelnde Enzyme aus Mikroorganismen. V. Reinigung einer 4‐En‐3‐oxosteroid: (Akzeptor)‐1‐en‐oxidoreductase aus <i>Nocardia opaca</i> und Charakterisierung der prosthetischen Gruppe

Lestrovaja, N.N.; Dänhardt, S.; Hörhold, C.

doi:10.1002/jobm.19780180306

Cited by 5 publications

(2 citation statements)

References 18 publications

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“…The strain is very active in transforming steroid compounds and is able to produce an inducible SdhA. The strain was cultivated and the SdhA w a s induced with 17-alphamethyl testosterone as described previously (LESTROVAYA et d. 1979).…”

Section: Methodsmentioning

confidence: 99%

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Localization of the steroid 1‐dehydrogenase in Rhodococcus erythropolis IMET 7030 by immunoelectron microscopy

Wagner¹,

Atrat²,

Clark‐Curtiss

et al. 1992

J. Basic Microbiol.

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The steroid 1-dehydrogenase of Rhodococcus erythropolis IMET 7030, an active steroid-transforming strain, was localized by immunogold labelling both in cells induced with 17-alpha-methyl-testosterone and in noninduced cells. The labelling intensity was much higher in induced cells than in noninduced cells, indicating increased enzyme production in the case of induction. Using the postembedding procedure, the main portion of the enzyme was found in the peripheral region of the cytoplasm. A considerable amount was bound in clusters to the inner side of the cytoplasmic membrane. The enzyme was also detected in channels connecting the cytoplasm with the cell surface. By means of the preembedding labelling, a few gold clusters could be detected on the cell surface. The significance of this observation was discussed.

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Section: Methodsmentioning

confidence: 99%

“…SdhA from Rhodococcus erj7t~ropo~js IMET 7030 has been extensively investigated by ATRAT et ui. (1977( ) and LESTROVAYA et a/. (1979.…”

mentioning

confidence: 99%

Localization of the steroid 1‐dehydrogenase in Rhodococcus erythropolis IMET 7030 by immunoelectron microscopy

Wagner¹,

Atrat²,

Clark‐Curtiss

et al. 1992

J. Basic Microbiol.

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Steroidumwandelnde Enzyme aus Mikroorganismen IX. Affinitätschromatographische Präparation und Untersuchung des Apoenzyms einer 4‐En‐3‐oxosteroid: (Akzeptor)‐1‐en‐oxidoreduktase aus Nocardia opaca

Atrat

Deppmeyer

Hörhold

et al. 1979

J of Basic Microbiology

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prosthetic group and apoenzyme were separated quantitatively by means of affinity chromatography in the presence of 2 M (NH,),SO, at p H 3.0. Subsequently the apoenzyme was eluted from affinity matrix by 0.01 M phosphate buffer, pH 8.0, whereas under these conditions the intact enzyme could not be eluted. The whole enzyme activity applied could be restored by incubation of the eluted apoenzyme with FAD. The binding strength of the apoenzyme to the immobilized steroid ligand is highly decreased in comparison to the native enzyme and can be interpreted by the action of rest hydrophobicity. That indicates the essential character of FAD for both ligand binding and transformation.

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Steroidumwandelnde Enzyme aus Mikroorganismen XI. Reversibilität der Dehydrierungsreaktion der Steroid‐1‐Dehydrogenase aus Nocardia opaca

Groh

Komel

Deppmeyer

et al. 1979

J of Basic Microbiology

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Highly purified preparations of the 4‐en‐3‐oxosteroid: (acceptor)‐1‐en‐oxidoreductase from Nocardia opaca have been investigated in both types of reactions: 1.2‐dehydrogenation of the 4‐en‐3‐oxo‐derivative and 1.2‐hydrogenation of the 1.4‐dien‐3‐oxo‐derivative. It was not possible to separate the hydrogenating activity from the dehydrogenating activity by affinity chromatography, disc electrophoresis, SDS‐electrophoresis, and isoelectric focusing techniques. The pure enzyme preparation is discussed as only one FAD depending protein acting in response to the system as a dehydrogenase as well as a reductase.

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Steroidumwandelnde Enzyme aus Mikroorganismen. V. Reinigung einer 4‐En‐3‐oxosteroid: (Akzeptor)‐1‐en‐oxidoreductase aus Nocardia opaca und Charakterisierung der prosthetischen Gruppe

Cited by 5 publications

References 18 publications

Localization of the steroid 1‐dehydrogenase in Rhodococcus erythropolis IMET 7030 by immunoelectron microscopy

Localization of the steroid 1‐dehydrogenase in Rhodococcus erythropolis IMET 7030 by immunoelectron microscopy

Steroidumwandelnde Enzyme aus Mikroorganismen IX. Affinitätschromatographische Präparation und Untersuchung des Apoenzyms einer 4‐En‐3‐oxosteroid: (Akzeptor)‐1‐en‐oxidoreduktase aus Nocardia opaca

Steroidumwandelnde Enzyme aus Mikroorganismen XI. Reversibilität der Dehydrierungsreaktion der Steroid‐1‐Dehydrogenase aus Nocardia opaca

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