Steroids and genes related to steroid biosynthesis in the female giant freshwater prawn, Macrobrachium rosenbergii

Thongbuakaew, Tipsuda; Siangcham, Tanapan; Suwansa‐ard, Saowaros; Elizur, Abigail; Cummins, Scott F.; Sobhon, Prasert; Sretarugsa, Prapee

doi:10.1016/j.steroids.2016.01.006

Cited by 41 publications

(27 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The E 2 and P 4 -like were first discovered in crustaceans in 1978 [56]. Furthermore, the existence of E 2 /P 4 in hepatopancreas, ovary and haemolymph, and the presence and activities of steroid-related enzymes (20α-HSD, 17β-HSD, 3β-HSD, 17β-HSD8) were recently reported in crustaceans, by which vitellogenesis was controlled [57][58][59][60][61][62]. Temporal expression profiles of Spot/vp and Spot/vpr-like mRNA showed that they might be involved in inhibiting ovarian development.…”

Section: Discussionmentioning

confidence: 98%

Role of Oxytocin/Vasopressin-Like Peptide and Its Receptor in Vitellogenesis of Mud Crab

Lin

Wei

2020

IJMS

View full text Add to dashboard Cite

Oxytocin (OT)/vasopressin (VP) signaling system is important to the regulation of metabolism, osmoregulation, social behaviours, learning, and memory, while the regulatory mechanism on ovarian development is still unclear in invertebrates. In this study, Spot/vp-like and its receptor (Spot/vpr-like) were identified in the mud crab Scylla paramamosain. Spot/vp-like transcripts were mainly expressed in the nervous tissues, midgut, gill, hepatopancreas, and ovary, while Spot/vpr-like were widespread in various tissues including the hepatopancreas, ovary, and hemocytes. In situ hybridisation revealed that Spot/vp-like mRNA was mainly detected in 6–9th clusters in the cerebral ganglion, and oocytes and follicular cells in the ovary, while Spot/vpr-like was found to localise in F-cells in the hepatopancreas and oocytes in the ovary. In vitro experiment showed that the mRNA expression level of Spvg in the hepatopancreas, Spvgr in the ovary, and 17β-estradiol (E2) content in culture medium were significantly declined with the administration of synthetic SpOT/VP-like peptide. Besides, after the injection of SpOT/VP-like peptide, it led to the significantly reduced expression of Spvg in the hepatopancreas and subduced E2 content in the haemolymph in the crabs. In brief, SpOT/VP signaling system might inhibit vitellogenesis through neuroendocrine and autocrine/paracrine modes, which may be realised by inhibiting the release of E2.

show abstract

Section: Discussionmentioning

confidence: 98%

Role of Oxytocin/Vasopressin-Like Peptide and Its Receptor in Vitellogenesis of Mud Crab

Lin

Wei

2020

IJMS

View full text Add to dashboard Cite

show abstract

“…Interestingly, the association between autophagy and reproduction in the invertebrate was recently described by Barth et al (2011), who suggested that the autophagy process induced by starvation could play a role in oogenesis in D. melanogaster . Recently, our group had identified estradiol and progesterone through LC-MS/MS analysis of the central nervous system, hepatopancreas and ovary of M. rosenbergii (Thongbuakaew et al, 2016b). In female prawn, progesterone stimulates ovarian maturation and spawning, whereas estradiol triggered yolk protein systhesis (Summavielle et al, 2003; Martins et al, 2007; Coccia et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…In female prawn, progesterone stimulates ovarian maturation and spawning, whereas estradiol triggered yolk protein systhesis (Summavielle et al, 2003; Martins et al, 2007; Coccia et al, 2010). Moreover, gene mining analysis found estrogen- and progesterone- like receptors in the eyestalk, central nervous system, and ovary of M. rosenbergii (Thongbuakaew et al, 2016b). The expression of estrogen receptors in ovarian cells was increased in caloric restriction every other day in female mice (Sluczanowska-Glabowska et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Starvation Promotes Autophagy-Associated Maturation of the Ovary in the Giant Freshwater Prawn, Macrobrachium rosenbergii

et al. 2017

Self Cite

View full text Add to dashboard Cite

Limitation of food availability (starvation) is known to influence the reproductive ability of animals. Autophagy is a lysosomal driven degradation process that protects the cell under metabolic stress conditions, such as during nutrient shortage. Whether, and how starvation-induced autophagy impacts on the maturation and function of reproductive organs in animals are still open questions. In this study, we have investigated the effects of starvation on histological and cellular changes that may be associated with autophagy in the ovary of the giant freshwater prawn, Macrobachium rosenbergii. To this end, the female prawns were daily fed (controls) or unfed (starvation condition) for up to 12 days, and the ovary tissue was analyzed at different time-points. Starvation triggered ovarian maturation, and concomitantly increased the expression of autophagy markers in vitellogenic oocytes. The immunoreactivities for autophagy markers, including Beclin1, LC3-II, and Lamp1, were enhanced in the late oocytes within the mature ovaries, especially at the vitellogenic stages. These markers co-localized with vitellin in the yolk granules within the oocytes, suggesting that autophagy induced by starvation could drive vitellin utilization, thus promoting ovarian maturation.

show abstract

“…In mollusks, TBT-induced imposex correlates with increased free testosterone (T) levels, probably induced by inhibition of acyl-CoA:testosterone acyltransferase, which conjugates T with fatty acids, and/or CYPs, reducing T clearance ( 19 , 93 ). The stimulatory effects of steroids on crustacean reproduction are well recognized; however, it was only with the development of modern omics technology that genes of steroidogenic enzymes and putative steroid receptors were identified ( 31 , 39 , 94 – 98 ). In female M. rosenbergii , TBT reduced 17β-estradiol in the hemolymph and ovary and increased T levels in the ovary ( 88 ), while in males, TBT reduced T levels in testis ( 73 , 90 ) (Figure 2 ) ( 53 , 94 ).…”

Section: Ots Effects On Reproductionmentioning

confidence: 99%

“…The stimulatory effects of steroids on crustacean reproduction are well recognized; however, it was only with the development of modern omics technology that genes of steroidogenic enzymes and putative steroid receptors were identified ( 31 , 39 , 94 – 98 ). In female M. rosenbergii , TBT reduced 17β-estradiol in the hemolymph and ovary and increased T levels in the ovary ( 88 ), while in males, TBT reduced T levels in testis ( 73 , 90 ) (Figure 2 ) ( 53 , 94 ). In crustaceans, an alternative action proposed was that TBT could block T excretion, but results are still inconclusive ( 18 , 93 , 99 , 100 ).…”

Section: Ots Effects On Reproductionmentioning

confidence: 99%

Effects of Organotins on Crustaceans: Update and Perspectives

2018

View full text Add to dashboard Cite

Organotins (OTs) are considered some of the most toxic chemicals introduced into aquatic environments by anthropogenic activities. They are widely used for agricultural and industrial purposes and as antifouling additives on boat hull’s paints. Even though the use of OTs was banned in 2008, elevated levels of OTs can still be detected in aquatic environments. OTs’ deleterious effects upon wildlife and experimental animals are well documented and include endocrine disruption, immunotoxicity, neurotoxicity, genotoxicity, and metabolic dysfunction. Crustaceans are key members of zooplankton and benthic communities and have vital roles in food chains, so the endocrine-disrupting effects of tributyltin (TBT) on crustaceans can affect other organisms. TBT can disrupt carbohydrate and lipid homeostasis of crustaceans by interacting with retinoid X receptor (RXR) and crustacean hyperglycemic hormone (CHH) signaling. Moreover, it can also interact with other nuclear receptors, disrupting methyl farnesoate and ecdysteroid signaling, thereby altering growth and sexual maturity, respectively. This compound also interferes in cytochrome P450 system disrupting steroid synthesis and reproduction. Crustaceans are also important fisheries worldwide, and its consumption can pose risks to human health. However, some questions remain unanswered. This mini review aims to update information about the effects of OTs on the metabolism, growth, and reproduction of crustaceans; to compare with known effects in mammals; and to point aspects that still needs to be addressed in future studies. Since both macrocrustaceans and microcrustaceans are good models to study the effects of sublethal TBT contamination, novel studies should be developed using multibiomarkers and omics technology.

show abstract

Steroids and genes related to steroid biosynthesis in the female giant freshwater prawn, Macrobrachium rosenbergii

Cited by 41 publications

References 56 publications

Role of Oxytocin/Vasopressin-Like Peptide and Its Receptor in Vitellogenesis of Mud Crab

Role of Oxytocin/Vasopressin-Like Peptide and Its Receptor in Vitellogenesis of Mud Crab

Starvation Promotes Autophagy-Associated Maturation of the Ovary in the Giant Freshwater Prawn, Macrobrachium rosenbergii

Effects of Organotins on Crustaceans: Update and Perspectives

Contact Info

Product

Resources

About