Steroidogenic effect of adenosine in the rat

Formento, M. L.; Borsa, M; Zoni, G

doi:10.1016/0031-6989(75)90024-7

Cited by 9 publications

(4 citation statements)

References 9 publications

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“…Adenosine did not stimu¬ late perfused normal rat adrenal cells (Fig. 6), in agreement with the results of others in vitro (Cooper & Gleed, 1978) and in vivo (Formento, Borsa & Zoni, 1975). In the isolated perfused rat adrenal gland, how¬ ever, adenosine had a profound effect in increasing flow through the gland, and also in stimulating corticosterone secretion, although it had no effect on aldosterone secretion (Fig.…”

Section: Response To Adrenalinesupporting

confidence: 92%

The relationship between perfusion medium flow rate and steroid secretion in the isolated perfused rat adrenal gland in situ

Hinson¹,

Vinson²,

Whitehouse³

1986

Journal of Endocrinology

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Using the in-situ, isolated, perfused rat adrenal preparation, we have investigated the effects of changes in the rate of perfusate flow through the gland, brought about both mechanically and by the use of vasoactive agents, in the absence of known adrenocortical stimulants. Adenosine caused a significant increase in the rate of perfusate flow through the adrenal, with a concomitant rise in corticosterone, but not aldosterone, secretion. Adrenaline, on the other hand, caused a decrease in the rate of perfusate flow through the gland, accompanied by a decrease in the rate of steroid secretion. Furthermore, increases in the rate of delivery of perfusate to the gland, brought about by increasing the peristaltic pump rate, caused a large increase in corticosterone secretion, although aldosterone was unaffected. Neither adenosine nor a mechanically increased rate of perfusate delivery increased steroid secretion by collagenase-dispersed rat adrenocortical cells superfused on a Sephadex column. These results suggest the existence of hitherto unsuspected intraglandular mechanisms for the control of steroid secretion.

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Section: Response To Adrenalinesupporting

confidence: 92%

The relationship between perfusion medium flow rate and steroid secretion in the isolated perfused rat adrenal gland in situ

Hinson¹,

Vinson²,

Whitehouse³

1986

Journal of Endocrinology

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“…The differences noted between the in vitro and in vivo effects of EtOH are most likely due to the incomplete metabolism of EtOH under in vitro conditions versus in vivo. The current experiment and past studies (15,16,18,19) suggest that purine metabolism and the resultant metabolites (ADO and URA), which are increased during in vivo EtOH metabolism, are involved in the regulation of EtOH's effect on the hypothalamic-pituitary-adrenal axis.…”

Section: Discussionmentioning

confidence: 60%

“…Adenosine (ADO) and its metabolites have been shown to play an important role in the regulation of many physiological processes (12)(13)(14), one of which is ACTHcorticosterone signaling (15,16). ADO occupies a central position in purine metabolism and has two primary metabolic pathways: a) reincorporation into the adenine nucleotide pool by phosphorylation or b) deamination to inosine and its subsequent metabolites (17).…”

mentioning

confidence: 99%

“…ADO occupies a central position in purine metabolism and has two primary metabolic pathways: a) reincorporation into the adenine nucleotide pool by phosphorylation or b) deamination to inosine and its subsequent metabolites (17). ADO (15,16), uric acid (URA), and other purine metabolites (18,19) have been shown to increase plasma ACTH and corticosterone concentration (PCC) significantly in rats. The interaction between EtOH, ADO, and its catabolic metabolites and the hypothalamic-pituitary-adrenal axis (HPAA) remains unclear.…”

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confidence: 99%

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Ethanol's Effect on Rat Pituitary Adrenal Axis is Prevented by Purine Metabolic Pathway Inhibitors

Szabó

Bruckner

Medveczky

et al. 1999

Proc Soc Exp Biol Med

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This study investigated the in vitro dose effects of ethanol (EtOH), adenosine (ADO), and urate (URA) on the basal and CRF stimulated ACTH production of pituitary tissue culture (PTC). Furthermore, the effects of low (LE = 0.5 g/kg bodyweight) and high (HE = 2.5 g/kg bodyweight) concentrations of EtOH were tested in rats on plasma ACTH and corticosterone concentration (PCC), with or without the following ADO metabolic pathway inhibitors: 6-mercaptopurine (6MP) (salvage pathway) and purpurogallin (PPG) (xanthine dehydrogenase). EtOH at 0-50 mM does not increase the in vitro basal or CRF (10-7 M) stimulated ACTH secretion in PTC; in fact doses up to 20 mM tended to be inhibitory. ADO significantly increased only basal ACTH secretion whereas URA increased both basal and CRF-stimulated ACTH secretion. Pretreatment with PPG or 6MP + PPG significantly increased both in vivo ACTH and PCC over control values in rats. HE versus LE significantly increased ACTH and PCC in the control (H2O) and 6MP pretreated groups whereas in the PPG pretreated animals, only ACTH was increased significantly by HE. However, combined pretreatment with 6MP + PPG prevented the effect of HE on ACTH and PCC. The current experiment suggests that purine metabolism is involved in ethanol's effect on the hypothalamic-pituitary-adrenal axis.

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Influence of hydrocortisone on the activity of 5?-nucleotidase and adenosine deaminase in the hypothalamus and hippocampus of the rat brain

VIa

1991

Neurosci Behav Physiol

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Steroidogenic effect of adenosine in the rat

Cited by 9 publications

References 9 publications

The relationship between perfusion medium flow rate and steroid secretion in the isolated perfused rat adrenal gland in situ

The relationship between perfusion medium flow rate and steroid secretion in the isolated perfused rat adrenal gland in situ

Ethanol's Effect on Rat Pituitary Adrenal Axis is Prevented by Purine Metabolic Pathway Inhibitors

Influence of hydrocortisone on the activity of 5?-nucleotidase and adenosine deaminase in the hypothalamus and hippocampus of the rat brain

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