Steroidal regulation of uterine immune defenses

Lewis, Gregory S.

doi:10.1016/j.anireprosci.2004.04.026

Cited by 94 publications

(84 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Sin embargo, es importante mencionar que actualmente no existe un consenso sobre un tratamiento efectivo para la CITO. El uso de PGF2á durante el diestro da como resultado la luteólisis y, posteriormente, el estro, que aumenta la inmunidad local mediante la eliminación del efecto inmunosupresor de la progesterona [95,96]. Además, la PGF2á posee un efecto ecbólico por el cual efectúa la eliminación de bacterias y debris [97]; sin embargo, solo un estudio mostró un beneficio En dos amplios estudios de campo realizados significativo del tratamiento con PGF2á [98], por nuestro grupo de investigación, utilizando el mientras que otros estudios no [38,97].…”

Section: Referenciaunclassified

Diagnosis and Treatment of Postpartum Uterine Diseases in Dairy Cows: A Review With Emphasis on Subclinical Endometritis

Pascottini

Opsomer

2017

Compend. cienc. vet

View full text Add to dashboard Cite

ABSTRACT. In this review, updated and precise definitions of the most common postpartum uterine diseases in dairy cows are provided. An aberrant uterine environment at inappropriate stages of the reproductive cycle inflicts damage to gametes and zygotes, impairing the reproductive performance of dairy cows. This involves major economic losses for the milk production unit. Consequently, an accurate diagnosis of postpartum uterine diseases is indispensable for practitioners to set up a prompt and efficient treatment. This review furthermore emphasizes on the new perspectives regarding diagnosis and treatment of subclinical endometritis, a highly prevalent uterine disease that is often overlooked by practitioners while causing major reproductive problems. Based on a more profound clinical understanding of the postpartum uterine disease complex, practitioners will be able to better use the available diagnostic tools and therefore apply a more efficient therapeutic approach. RESUMEN. El objetivo de este artículo de revisión es proporcionar definiciones actualizadas y precisas de las enfermedades uterinas posparto más comunes en vacas lecheras. Un ambiente uterino hostil en etapas inapropiadas del ciclo reproductivo inflige daño a los gametos y cigotos, lo que perjudica el rendimiento reproductivo de las vacas lecheras. En este contexto, una menor fertilidad contribuye negativamente al éxito económico de la unidad de producción lechera. En consecuencia, un diagnóstico adecuado de la enfermedad del útero en el posparto es indispensable para que los profesionales veterinarios puedan establecer un tratamiento con prontitud y eficacia. Esta revisión hace hincapié en las nuevas perspectivas de diagnóstico y tratamiento de la endometritis subclínica, una patología uterina de alta prevalencia que a menudo los profesionales pasan por alto. Así, con una profunda comprensión clínica del complejo de enfermedades del posparto uterino, podrán utilizarse mejor las herramientas de diagnóstico disponibles y por lo tanto aplicar un enfoque terapéutico más eficiente. KeywordsPalabras clave: vacas lecheras, endometritis, citología

show abstract

Section: Referenciaunclassified

Diagnosis and Treatment of Postpartum Uterine Diseases in Dairy Cows: A Review With Emphasis on Subclinical Endometritis

Pascottini

Opsomer

2017

Compend. cienc. vet

View full text Add to dashboard Cite

show abstract

“…Neutrophils, which are involved in the initial attack against bacteria entering the uterus (Targowski 1984, Lewis 2004, attacked the shell-free conceptus, providing the first experimental evidence that the marsupial shell coat provides an immunoprotective barrier between the conceptus and the maternal immune system. Although shell-free conceptuses are able to survive in vitro during cleavage (Moore & Taggart 1993, Renfree & Lewis 1996, Casey & Selwood 2003, the presence of neutrophils attacking the shell-free conceptus in vivo shown here and in vitro conceptus failure during blastocyst formation after shell removal (Casey & Selwood 2003), show that the long-term survival of a conceptus would be compromised in GST-CP4-immunized animals.…”

Section: Uterine Secretions As Immunocontraceptive Targetsmentioning

confidence: 99%

“…However, in eutherians, semen, the conceptus (Robertson 2000), progesterone (Piccinni 2003, Lewis 2004) and in mice, early pregnancy factor (EPF; Morton 1998) induce the expression of specific cytokines able to skew the differentiation and expansion of certain lymphocyte populations, suppressing the immune response. Further immunization during oestrus results in a lower immunoglobulin response than at other times in both rats (Wira & Rossoll 1995) and mice (Gockel et al 2000).…”

Section: Major Histocompatibility Class (Mhc) IImentioning

confidence: 99%

Novel immunocontraceptive targets in mammals: uterine secretions and the conceptus; a marsupial approach

Menkhorst¹,

Cui²,

Selwood³

2008

Reproduction

View full text Add to dashboard Cite

We report the first immunocontraceptive trial in mammals using a uterine-secreted protein, the marsupial shell coat protein 4 (CP4). The marsupial shell coat, which surrounds the conceptus for 60-80% of gestation, is secreted by the uterine epithelium. Following immunization against glutathione S-transferase (GST)-CP4, the fertility of female common brushtail possums (nZ6) was significantly reduced (PZ0.000), and this reduction in fertility was positively correlated with the maximum GST-CP4 humoral immune response (PZ0.025). Ultrastructural examination of the reproductive tract indicated that the cell-mediated immune response against GST-CP4 targeted the shell coat, the shell-free conceptus and the uterine glandular epithelium, thus preventing normal conceptus development and uterine secretion of shell coat proteins and nutrients. These results show that uterine-secreted proteins are promising immunocontraceptive targets, especially in pest mammal species, e.g. possum, rabbit and horse, that have uterine-secreted additions to embryonic coats, or that have late implantation requiring uterine nutrient provisioning from secretions.

show abstract

“…Such alterations in ghrelin action could be due to the initial state of ovarian cells affected by season, nutrition and other environmental factors. P 4 , OXT and PGF are known to control of ovarian luteogenesis, uterine contractions and the immune response (Mitchell & Schmid 2001, Lewis 2004, while steroid hormones and IGF1 are known promoters of ovarian follicullogenesis (Hillier 2000, Berisha & Schams 2005. It is possible that ghrelin acting through P 4 , OXT, IGF1, steroids and PGF mediates effect of nutrition on these processes.…”

Section: Effect Of Ghrelin and Stat1 On Ovarian Cellsmentioning

confidence: 99%

“…These observations demonstrate that STAT1 can inhibit ovarian functions, acting as a suppressor of ovarian follicular cell proliferation and, perhaps, of resulting follicular growth, and as a promoter of ovarian cell apoptosis and resulting follicular atresia. Furthermore, STAT1 can stimulate ovarian PGF and OXT, antiluteal and anti-conception factors (Wathes 1989, Mitchell & Schmid 2001, Lewis 2004. Therefore, STAT1 can be considered to be a negative regulator of reproductive processes and mediator of unknown extracellular factors inhibiting these processes.…”

Section: Effect Of Ghrelin and Stat1 On Ovarian Cellsmentioning

confidence: 99%

Involvement of the transcription factor STAT1 in the regulation of porcine ovarian granulosa cell functions treated and not treated with ghrelin

Benco

Sirotkin²,

Vašíček³

et al. 2009

Reproduction

View full text Add to dashboard Cite

The aim of our in vitro experiments was to study the role of the transcription factor STAT1 and the hormone ghrelin in controlling porcine ovarian function. The effects of treatment with ghrelin (0, 1, 10, 100 ng/ml), transfection-induced overexpression of transcription factor STAT1, and their combination on apoptosis (expression of apoptosis-related peptides caspase-3, BAX and anti-apoptotic peptide BCL2), proliferation (expression of proliferating cell nuclear antigene PCNA, proliferation-associated protein kinase MAPK/ERK1,2) and release of the hormones progesterone (P 4 ), prostaglandin F (PGF) and oxytocin (OXT) in cultured porcine ovarian granulosa cells was evaluated using RIA, immunocytochemistry and SDS-PAGE-western immunoblotting. It was found that ghrelin, when given alone, increased the expression of proliferation-associated PCNA and MAPK/ERK1,2, decreased the accumulation of apoptosis-related substances caspase-3, BAX, BCL2, decreased P 4 , and increased PGF and OXT release. Ghrelin tended to promote accumulation of STAT1 in both control and transfected cells, although in transfected cells ghrelin at 1 ng/ml decreased STAT1 accumulation. Transfection of porcine granulosa cells by a gene construct encoding STAT1 promoted the expression of STAT1 and apoptosis-related-BAX but the expression of BCL2 did not, and decreased the accumulation of proliferation-associated MAPK/ERK1,2 but not that of PCNA. It also promoted PGF and OXT but not P 4 release. Overexpression of STAT1 reversed the effect of ghrelin on STAT1, PCNA, PGF, OXT (from stimulatory to inhibitory), BCL2, P 4 (from inhibitory to stimulatory), prevented ghrelin effect on caspase-3 and BAX, but did not affect ghrelin's effect on MAPK/ERK1,2 expression. These results suggest that ghrelin directly affects porcine ovarian cells function -stimulates proliferation, inhibits apoptosis and affects secretory activity. Furthermore, they demonstrated the involvement of the transcription factor STAT1 in controlling these functions, the promotion of some markers of apoptosis (BAX), inhibition of some markers of proliferation (MAPK/ERK1,2) and stimulation of PGF release. Finally, the obtained data failed to demonstrate that STAT1 is involved in mediating the action of ghrelin on ovarian cell functions.

show abstract

Steroidal regulation of uterine immune defenses

Cited by 94 publications

References 62 publications

Diagnosis and Treatment of Postpartum Uterine Diseases in Dairy Cows: A Review With Emphasis on Subclinical Endometritis

Diagnosis and Treatment of Postpartum Uterine Diseases in Dairy Cows: A Review With Emphasis on Subclinical Endometritis

Novel immunocontraceptive targets in mammals: uterine secretions and the conceptus; a marsupial approach

Involvement of the transcription factor STAT1 in the regulation of porcine ovarian granulosa cell functions treated and not treated with ghrelin

Contact Info

Product

Resources

About