Steroidal glycosides from the bulbs of Bessera elegans and their cytotoxic activities

Steroidal Glycosides from Convallaria majalis Whole Plants and Their Cytotoxic Activity

“…Compound 8 , which is the corresponding furostanol glycoside of 1 , was only cytotoxic to the adherent cell lines of A549, HSC-4, and HSC-2 cells, with IC 50 values of 2.97 ± 0.06 μM, 11.04 ± 0.25 μM, and 8.25 ± 0.20 μM, respectively ( Table 3 ). As we previously reported [ 11 , 12 ], the cytotoxicity of 1 compared to those of 2 , 4 , 5 , and 6 and of 8 compared to those of 9 and 10 indicated that the introduction of polar substituents to the steroidal nuclei resulted in reduced the cytotoxicity. Compound 1 was cytotoxic to tumor cells, whereas 3 , having the diglycoside did not show cytotoxic activity.…”

“…Cell cycle distribution was evaluated using a flow cytometer (FACSCanto II, BD Bioscience, San Jose, CA, USA). A549 cells (1 × 10 5 cells/ml) were treated with 8 (20 μM) for 30 h, in separate experiments, according to a previously reported method [ 11 ]. The DNA contents of the tumor cells were analyzed, and percentages of cells in each phase were calculated.…”

An efficient approach to identify different chemical markers between fibrous root and rhizome of Anemarrhena asphodeloides by ultra high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry with multivariate statistical analysis

“…With the help of intact precursor ion in the low CE ESI-, the molecular formula could be obtained; with the help of characteristic fragment ions in the high CE ESI+ and ESI−, the number, connection order and classes of sugar units could be deduced. According to previous phytochemical study on the rhizome of A. asphodeloides, the sugar units linking to the C-3 and C-26 of the aglycone were usually made up of D-galactose, D-xylose, D-glucose and L-rhamnose [7,[14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32]. In addition, the retention time was associated with the structures of steroidal saponins.…”

Steroidal and Triterpenoid Glycosides from Roots of Digitalis ciliata

“…22))), three secondary alcohol functions (d(C) 71.5 (C(2)), 84.5 (C(3)), and 85.3 (C(16))), and one primary alcohol function (d(C) 75.5 (C(26))), indicating the presence of a glycosidic furostanol skeleton. On the basis of the HSQC correlations and HMBCs and comparison with the literature data, the aglycon moiety of compound 1 was identified as (25R)-5a-furost-20(22)-ene-2a,3b,26-triol [7]. The configuration at C(25) was deduced to be (R) based on the difference of chemical shifts…”