Steroidal fraction of<i>Carissa carandas</i>L. inhibits microbial hyaluronidase activity by mixed inhibition mechanism

Patil, Sandip P.; Bhadane, Bhushan S.; Shirsath, Leena P.; Patil, Ravindra H.; Chaudhari, Bhushan L.

doi:10.1080/10826068.2018.1541811

Cited by 11 publications

(4 citation statements)

References 53 publications

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“…As a virulence factor, it exerts toxicity on the host by decomposing various types of proteins, such as collagen, fibrinogen, and complement, which are involved in immunity. It is also known to cause problems, such as disturbance of the host immune system and induction of endocarditis, mainly because of its extensive proteolytic activity [ 40 , 41 ].…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Safety and Probiotic Potential of Enterococcus faecalis MG5206 and Enterococcus faecium MG5232 Isolated from Kimchi, a Korean Fermented Cabbage

Kim¹,

Choi²,

Jeong³

et al. 2022

Microorganisms

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The purpose of this study was to evaluate the genotypic and phenotypic toxicity of Enterococcus faecalis MG5206 and Enterococcus faecium MG5232 isolated from kimchi (fermented vegetable cabbage). In this study, the genotypic toxicity of the strains MG5206 and MG5232 was identified through whole-genome sequencing analysis, and phenotypic virulence, such as susceptibility to antibiotics, hemolytic activity, and gelatinase and hyaluronidase activities, was also evaluated. In addition, the in vivo toxicity of both strains was evaluated using an acute oral administration test in Sprague–Dawley rats. In all the tests, both the strains were determined to be safety by confirming that they did not show antibiotic resistance or virulence factors. In addition, these strains exhibited a low level of autoaggregation ability (37.2–66.3%) and hydrophobicity, as well as a high survival rate in gastrointestinal condition in vitro. Therefore, the safety and high gastrointestinal viability of E. faecalis MG5206 and E. faecium MG5232 suggests that both the strains could be utilized in food as potential probiotics in the future.

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Section: Resultsmentioning

confidence: 99%

Evaluation of Safety and Probiotic Potential of Enterococcus faecalis MG5206 and Enterococcus faecium MG5232 Isolated from Kimchi, a Korean Fermented Cabbage

Kim¹,

Choi²,

Jeong³

et al. 2022

Microorganisms

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“…Analysis using CLEAN (contrastive learning-enabled enzyme annotation), which predicts enzyme function (Gregoire et al 2023), showed that one NTF-specific OG with the polysaccharide lyase family 8 domain is closely related to Hyl, a bacterial hyaluronate lyase (EC:4.2.2.1). Bacterial Hyl is an important virulence factor employed by Gram-positive bacteria to enhance their infectivity by degrading extracellular hyaluronate and chondroitin sulfate of animal hosts (Patil et al 2023).…”

Section: Characteristics Of the Ntf Genomesmentioning

confidence: 99%

The Genomes of Nematode-Trapping Fungi Provide Insights into the Origin and Diversification of Fungal Carnivorism

Fan,

Du,

Zhang

et al. 2024

Preprint

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Nematode-trapping fungi (NTF), most of which belong to a monophyletic lineage in Ascomycota, cannibalize nematodes and other microscopic animals, raising questions regarding the types and mechanisms of genomic changes that enabled carnivorism and adaptation to the carbon-rich and nitrogen-poor environment created by the Permian-Triassic extinction event. Here, we conducted comparative genomic analyses of 21 NTF and 21 non-NTF to address these questions. Carnivorism-associated changes include expanded genes for nematode capture, infection, and consumption (e.g., adhesive proteins, CAP superfamily, eukaryotic aspartyl proteases, and serine-type peptidases). Although the link between secondary metabolite (SM) production and carnivorism remains unclear, we found that the numbers of SM gene clusters among NTF are significantly lower than those among non-NTF. Significantly expanded cellulose degradation gene families (GH5, GH7, AA9, and CBM1) and contracted genes for carbon-nitrogen hydrolases (enzymes that degrade organic nitrogen to ammonia) are likely associated with adaptation to the carbon-rich and nitrogen-poor environment. Through horizontal gene transfer events from bacteria, NTF acquired the Mur gene cluster (participating in synthesizing peptidoglycan of the bacterial cell wall) and Hyl (a virulence factor in animals). Disruption of MurE reduced NTF's ability to attract nematodes, supporting its role in carnivorism. This study provides new insights into how NTF evolved and diversified after the Permian-Triassic mass extinction event.

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“…Hyaluronic acid (HA) is synthesized and secreted from the plasma membrane of mammalian cells, and it serves as a cementing structure of connective tissues and is also involved in water homeostasis, assisting with cell proliferation, and acting as an immune regulator [15] . Many of these tissues with high HA concentrations are frequently infected with S aureus due to their ability to produce hyaluronidase [16] .…”

Section: Main Virulence Factors Of S Aureusmentioning

confidence: 99%

Metal nanoparticles as inhibitors of enzymes and toxins of multidrug-resistant Staphylococcus aureus

Joshi,

Patil

2023

Infectious Medicine

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Steroidal fraction ofCarissa carandasL. inhibits microbial hyaluronidase activity by mixed inhibition mechanism

Cited by 11 publications

References 53 publications

Evaluation of Safety and Probiotic Potential of Enterococcus faecalis MG5206 and Enterococcus faecium MG5232 Isolated from Kimchi, a Korean Fermented Cabbage

Evaluation of Safety and Probiotic Potential of Enterococcus faecalis MG5206 and Enterococcus faecium MG5232 Isolated from Kimchi, a Korean Fermented Cabbage

The Genomes of Nematode-Trapping Fungi Provide Insights into the Origin and Diversification of Fungal Carnivorism

Metal nanoparticles as inhibitors of enzymes and toxins of multidrug-resistant Staphylococcus aureus

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