STEROID TRANSFORMATIONS BY CORPUS LUTEUM TISSUE<sup>1</sup>

Hayano, Mika; Lindberg, Marjorie C.; Wiener, Margaret; Rosenkrantz, Harris; Dorfman, Ralph I.

doi:10.1210/endo-55-3-326

Cited by 43 publications

(12 citation statements)

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“…1). The values (with standard error) for the average per cent of gonadotropic stimulation 14 C incorporation into progesterone in bovine corpora lutea slices. Each bar is the mean with standard error, expressed as per cent of gonadotropic stimulation.…”

Section: Resultsmentioning

confidence: 99%

“…for incorporation of acetate-l- 14 The response to PGE 2 was studied for varying doses. The data in Table 2 represent the results of experiments with seven corpora lutea.…”

Section: Resultsmentioning

confidence: 99%

“…Bovine luteal tissue produces two major progestin products, progesterone and 20/3-hydroxypregn-4-en-3-one (6,14,15). The reduced progestin (20/3-hydroxypregn-4-en-3-one) is a less active progestational agent (16), and preferential stimulation of this product could possibly result in a luteolytic effect (17).…”

Section: Discussionmentioning

confidence: 99%

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Prostaglandin Stimulation ofin VitroProgesterone Synthesis

Speroff¹,

Ramwell²

1970

The Journal of Clinical Endocrinology & Metabolism

179

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Bovine corpora lutea slices were incubated with prostaglandins and gonadotropins. All the prostaglandins tested (PGE 2 , PGE X , PGFjo and PGAi) were found to be steroidogenic, stimulating both the production of progesterone as measured in micrograms and the incorporation of radioactivity from acetate-l-u C. Prostaglandin E2 (PGE 2 ) gave the greatest effect, being approximately half as effective as luteinizing hormone (LH) on a molar basis. There were similarities between gonadotropin and prostaglandin. Cycloheximide (1 HIM) equally blocked the steroidogenic response to both PGE 2 and LH, the specific activities of the progesterone formed in the presence of prostaglandins and that formed in the presence of gonadotropins were approximately of the same order of magnitude, the time-response curves of PGE 2 and LH were similar, and there was no additive effect when prostaglandins were added to luteal slices incubated with saturating doses of either LH or human chorionic gonadotropin (HCG). These results are in contrast to the in vivo luteolytic effect of PGF 2 Q , and it is unlikely, therefore, that the luteolytic effect of PGF 2a is due to a direct inhibition of luteal steroidogenesis. Thus, the in vitro and in vivo effects of prostaglandins may represent 2 different mechanisms of action. (J Clin Endocr 30: 345, 1970)

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Section: Resultsmentioning

confidence: 99%

“…for incorporation of acetate-l- 14 The response to PGE 2 was studied for varying doses. The data in Table 2 represent the results of experiments with seven corpora lutea.…”

Section: Resultsmentioning

confidence: 99%

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Prostaglandin Stimulation ofin VitroProgesterone Synthesis

Speroff¹,

Ramwell²

1970

The Journal of Clinical Endocrinology & Metabolism

179

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“…Slaunwhite (66) and bovine (67) ovarian tissue in vitro, and by the eviscerated rat in vivo (68), and normally accompany progesterone in the blood of pregnant women and in human follicles, corpora lutea and possibly in placental blood (35).…”

Section: Biochemical Considerationsmentioning

confidence: 99%

Clinical, Morphological and Biochemical Studies of a Virilizing Tumor in the Testis*

Savard¹,

Dorfman²,

Baggett³

et al. 1960

J. Clin. Invest.

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(22,23). A few studies of the qualitative nature of the urinary steroids in patients with this type of tumor seem to confirm such a conclusion (13,24). Accordingly, when the opportunity arose to study a patient bearing a testicular -tumor thought to be of this type, it seemed worthwhile to attempt to establish the exact nature of the steroids produced. The study presented here was therefore undertaken.This paper presents a description of the patient, histological observations on the tumor, the results of urinary steroid assays and the results of experiments in which slices of the tumor were incubated with C14-labeled steroid precursors. EXPERIMENTAL AND RESULTS

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“…Natural occurrence of a second progestin, A4 pregnen-20P-ol-3-one ( Zop-ol), was first observed in bovine luteal tissue by Gorski et al (2). In vitro studies have also demonstrated the ability of these organs to reduce progesterone to 20P-o1 (3). In this investigation Concentrations of progesterone and 2Op-01 were determined in bovine corpora lutea, placenta and associated fluids and blood plasma at selected stages of the estrous cycle and pregnancy.…”

mentioning

confidence: 99%