Steroid Sulfates from Ophiuroids (Brittle Stars): Action on Some Factors of Innate and Adaptive Immunity

Abstract

The action of seven polyhydroxylated sterol mono-and disulfates (1-7), isolated from ophiuroids, on innate and adaptive immunity was examined in in vitro and in vivo experiments. At least, three of them (1, 2 and 4) increased the functional activities of neutrophils, including levels of oxygen-dependent metabolism, adhesive and phagocytic properties, and induced the expression of pro-inflammatory cytokines TNF- and IL-8. Compound 4 was the most active for enhancing the production of antibody forming cells in the mouse spleen.

Disulfated Ophiuroid Type Steroids from the Far Eastern Starfish Pteraster marsippus and Their Cytotoxic Activity on the Models of 2D and 3D Cultures

“…Nevertheless, the steroidal metabolites of ophiuroids were reported to inhibit the protein tyrosine kinase (PTK) [ 17 ], to show antiviral activity against HIV-1 and HIV-2 [ 18 ], and to be potent antagonists of farnesoid-X-receptor (FXR), a ligand-regulated transcription factor involved in the supporting of the lipid and glucose homeostasis in mammals [ 19 ]. These compounds enhanced oxygen-dependent metabolism, increased adhesive and phagocytic properties, induced the expression of pro-inflammatory cytokines TNF-α and IL-8 in neutrophils, and enhanced the production of antibody-forming cells in the mouse spleen [ 20 ]. Biological activities of steroidal disulfates from Pterasteridae starfish were less studied, but hemolytic activity on mouse erythrocytes was reported [ 15 ].…”