Steroid Receptors in Canine and Human Female Genital Tract Tumours with Smooth Muscle Differentiation

Millán, Y.; Gordon, Ana; Monteros, A. Espinosa de los; Reymundo, C.; Mulas, J. Martı́n de las

doi:10.1016/j.jcpa.2007.01.004

Cited by 23 publications

(26 citation statements)

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“…Calponin is a sensitive marker of smooth muscle differentiation in human neoplasm and shows expression of 89.3% and 100% in canine and human fibroid, respectively [17]. The higher expression of calponin in the resistant group might be explained as a result of active smooth muscle differentiation or proliferation which might surpass the inhibitory effects of GnRH-a.…”

Section: Discussionmentioning

confidence: 99%

Comparison of estrogen receptor-α, progesterone receptor and calponin expression in gonadotrophin-releasing hormone agonist-sensitive and -resistant uterine fibroids

Kim

Lee

et al. 2014

Obstet Gynecol Sci

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ObjectiveThis study was aimed to compare immunohistochemical expression of estrogen receptor (ER)-α, progesterone receptor (PR), and calponin in gonadotrophin-releasing hormone agonist (GnRH-a)-sensitive and -resistant uterine fibroids.MethodsWe collected data retrospectively. The sensitive group consisted of women who had reduction in uterine volume greater than 40% following GnRH-a treatment. Uterine volume was either reduced by less than 10%, or was increased in the resistant group. A tissue microarray was constructed using formalin-fixed, paraffin-embedded tissues, 31 and 26 patients for the sensitive and resistant groups, respectively. Tissue sections were immunostained with antibodies against ER-α, PR, and calponin. The intensity and area of the immunohistochemical reactions were evaluated using a semi-quantitative scoring system. The Mann-Whitney U-test, Fisher's exact test, and Spearman's rank correlation test were used for analysis of data.ResultsPR (P = 0.04) and calponin (P = 0.03) showed a significantly higher staining intensity in the resistant group than in the sensitive group. Both groups showed comparable expression of ER-α (P = 0.23). In correlation analysis between changes in uterine volume after GnRH-a therapy and clinicopathological factors, the immunohistochemical intensity of PR (P = 0.04) and calponin (P = 0.03) was significantly correlated with changes in uterine volume.ConclusionThis study shows that GnRH-a resistance of uterine fibroids is not related to ER-α content, but the expression of PR and calponin is related with GnRH-a resistance.

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Section: Discussionmentioning

confidence: 99%

Comparison of estrogen receptor-α, progesterone receptor and calponin expression in gonadotrophin-releasing hormone agonist-sensitive and -resistant uterine fibroids

Kim

Lee

et al. 2014

Obstet Gynecol Sci

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“…Em um estudo com 32 tumores genitais de cadela (leiomioma, ϐibroleiomioma e leiomiossarcoma), 56,3% apresentaram expressão para receptor de estrógeno tipo alfa e 84,4% para receptor de progesterona (Millán et al 2007). Essa característica pode possibilitar a realização de terapia hormonal nas cadelas acometidas.…”

Section: Discussionunclassified

“…Poucos trabalhos utilizaram TM para diferenciar e caracterizar esses neoplasmas (Munday & Stedman 2002, Manarolla et al 2011) e, nesses trabalhos, também se constatou que a coloração de TM é tão útil nesse diagnóstico quanto a IHQ. A IHQ com marcadores de músculo liso como a actina, a desmina e a calponina é amplamente utilizada para o diagnóstico desses neoplasmas (Cooper & Valentine 2002, Millán et al 2007, Manarolla et al 2011. Nesse trabalho foram utilizadas a vimentina e a desmina.…”

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Caracterização histopatológica e imuno-histoquímica de neoplasmas mesenquimais da genitália em 43 cadelas

et al. 2012

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Os arquivos de biópsia do período de 2000 a 2010 do SPV-UFRGS foram revisados e levantados os casos de neoplasias mesenquimais de genitália (útero, cérvix, vagina e vulva) de cadelas. Realizou-se estudo retrospectivo de 43 casos, incluindo caracterização histológica, coloração tricrômico de Masson (TM) e imuno-histoquímica (IHQ). As principais raças acometidas foram Cocker, Poodle e Pastor Alemão, porém o maior número de casos foi observado em cães sem raça definida (SRD). A idade média dessas cadelas foi de 10,6 anos (variação de 3 a 10 anos). Quanto à localização dos tumores, 44,2% estavam na vagina, 27,9% no útero, 23,3% na vulva e 4,6% na cérvix. Histologicamente, 46,5% eram leiomioma, 41,9% fibroleiomioma, 7,0% fibroma e 4,6% leiomiossarcoma. Na coloração de TM, os fibromas apresentaram todas as células neoplásicas coradas em azul (colágeno), os leiomiomas e os leiomiossarcomas apresentaram menos de 50% de colágeno em meio às células neoplásicas musculares e os fibroleiomiomas mais de 50%. Na IHQ, os fibromas apresentaram marcação positiva para vimentina e negativa para desmina. Todos os leiomiomas eram positivos para vimentina (em mais de 50% células) e para desmina (75% dos casos em mais de 50% das células e 25% em menos de 50%). Em um dos casos de leiomiossarcoma houve imunomarcação para vimentina e ausente para desmina e, no outro, ocorreu o oposto. Os fibroleiomiomas apresentaram marcação para vimentina em 94,4% (em mais de 50% células) e para desmina em 77,8% (64,3% em menos de 50% das células e 35,7% em mais de 50%).

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“…The tumours are usually seen in medium‐aged, non‐spayed, nulliparous dogs and surgery is the treatment of choice (Klein 2001). The majority of canine genital tract leiomyomas have progesterone receptors (Millán and others 2007), a finding that opens up the possibility of using progesterone receptor antagonists as a treatment for these tumours. The clinical and pathological findings of a vaginal fibroma with progesterone receptors that was treated with aglepristone (RU 534 Alizin; Virbac) before surgery to facilitate surgical access is reported here.…”

Section: Introductionmentioning

confidence: 99%

Effects of aglepristone, a progesterone receptor antagonist, in a dog with a vaginal fibroma

Rollón

Millán

Mulas

2007

J of Small Animal Practice

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A 12-year-old, entire, nulliparous crossbreed female dog was presented with a history of vulval bleeding, bulging of the perineum and faecal tenesmus. A firm, non-painful perineal mass, measuring 9.11x5.4 cm, with erythema was detected. Abdominal radiography showed compression and elevation of the rectal ampulla. A dose of 10 mg/kg aglepristone was administered subcutaneously on days 1, 2, 8, 15, 28 and 35. An incision biopsy was taken on day 15 and immunohistochemical analysis showed that the majority of neoplastic cells expressed progesterone receptors. Both the cutaneous erythema and the faecal tenesmus had resolved by day 28. A 50 per cent reduction in size was observed by day 60 (surgical excision). This study shows that benign tumours of the vagina of the dog that contain progesterone receptors can be reduced in size in a palliative or neoadjuvant setting using the progesterone receptor antagonist aglepristone.

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Steroid Receptors in Canine and Human Female Genital Tract Tumours with Smooth Muscle Differentiation

Cited by 23 publications

References 11 publications

Comparison of estrogen receptor-α, progesterone receptor and calponin expression in gonadotrophin-releasing hormone agonist-sensitive and -resistant uterine fibroids

Comparison of estrogen receptor-α, progesterone receptor and calponin expression in gonadotrophin-releasing hormone agonist-sensitive and -resistant uterine fibroids

Caracterização histopatológica e imuno-histoquímica de neoplasmas mesenquimais da genitália em 43 cadelas

Effects of aglepristone, a progesterone receptor antagonist, in a dog with a vaginal fibroma

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