Steroid productions by Co-cultures of granulosa cells with inner and outer theca cells in preovulatory follicles of gonadotropin stimulated calves

Bosc, M. J.; Nicolle, Agnès

doi:10.1016/s0960-0760(97)00030-7

Cited by 6 publications

(6 citation statements)

References 40 publications

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“…In scheme 2 where both cells were mixed and co-encapsulated in same capsule, the levels of progesterone levels increased with time indicating enhanced luteal differentiation of the endocrine cells over time. This may have been due to the over-crowding of cells in the capsules with time or mixing of both cell types, as both conditions are known to induce luteal differentiation, where biosynthesis of estrogen diminishes and progesterone increases [41;49]. The presence of FSH and E 2 has been reported not only to prevent ovarian cell atresia but also to maintain the aromatase activity during the long term culture.…”

Section: Discussionmentioning

confidence: 99%

Engineered multilayer ovarian tissue that secretes sex steroids and peptide hormones in response to gonadotropins

Sittadjody¹,

Saul²,

Joo³

et al. 2013

Biomaterials

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Although hormone replacement therapy is an option for the loss of ovarian function, hormone delivery through pharmacological means results in various clinical complications. The present study was designed to deliver sex steroids by a functional construct fabricated using encapsulation techniques. Theca and granulosa cells isolated from ovaries of 21-day old rats were encapsulated in multilayer alginate microcapsules to recapitulate the native follicular structure. Cells encapsulated in two other schemes were used as controls to assess the importance of the multilayer structure. The endocrine functions of the encapsulated cells were assessed in vitro for a period of 30 days. Encapsulated cells showed sustained viability during long-term in vitro culture with those encapsulated in multilayer capsules secreting significantly higher and sustained concentrations of 17 β-estradiol (E2) than the two other encapsulation schemes (p<0.05, n=6) in response to follicle-stimulating hormone (FSH) and luteinizing hormone (LH). In addition, cells in the multilayer microcapsules also secreted activin and inhibin in vitro. In contrast, when granulosa and theca cells were cultured in 2-D culture, progesterone (P4) secretion increased while E2 secretion decreased over a 30-day period. In summary, we have designed a multilayer engineered ovarian tissue that secretes sex steroids and peptide hormones and responds to gonadotropins, thus demonstrating the ability to recapitulate native ovarian structure ex vivo.

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Section: Discussionmentioning

confidence: 99%

Engineered multilayer ovarian tissue that secretes sex steroids and peptide hormones in response to gonadotropins

Sittadjody¹,

Saul²,

Joo³

et al. 2013

Biomaterials

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“…These results indicate that co-culture had different effects on steroidogenic mechanisms at three GC stages. Such different effects of TCs on GCs at different stages were also shown in mammals [8,24,45,46], but the trends were different from those in poultry. These results suggest that TCs might play a unique role in the regulation of GC in poultry.…”

Section: Discussionmentioning

confidence: 68%

“…In mammals, GCs and TCs together constitute the steroid synthesis system of follicles, and these roles in synthesis are their main physiological functions [7,8]. Follicle synthesis includes the production of various steroids, mainly progesterone, androgen and estrogen.…”

Section: Introductionmentioning

confidence: 99%

Exploration of the effects of goose TCs on GCs at different follicular stages using a co-culture model

et al. 2020

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Granulosa cells (GCs) play a critical role in follicular development, which cannot be separated from the assistance of theca cells (TCs). In this study, we used a transwell system to develop three stages of goose GCs in vitro mono-culture and co-culture models, and we analyzed the morphology, activity, intracellular lipid content and the expression of core genes involved in de novo lipogenesis (DNL), steroidogenesis, proliferation and apoptosis of the GCs. In the co-culture group, the activity of all three stages of GCs showed significant (P<0.01) changes, and they had a strong (P<0.01) correlation with culture time; further, the intracellular lipid deposition of hierarchical GCs was significantly different (P<0.01) between the two methods. Moreover, after co-culture, in pre-hierarchical GCs, the expression of SREBP, CYP11 and 3βHSD was promoted (P < 0.01). In hierarchical GCs, the expression of ACC, SREBP, STAR, CYP11, 3βHSD and CCND1 was promoted at 48 h, but they were inhibited (P < 0.05) at 96 h. In F1 GCs, the expression of ACC, FAS, SREBP, CYP11, BCL2 and CAS3 was inhibited (P < 0.01). The results indicate that goose TCs had complex and time-dependent effects on the biological function of GCs at each corresponding stage, and the effects were distinct in the different stages. In addition, DNL, steroidogenesis, proliferation and apoptosis in hierarchical and F1 GCs might have some synergistic relationships in the effects of TCs on GCs. Furthermore, we speculated that TCs might play an important role in the differentiation and maturation of GCs during follicular development.

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“…The intraand inter-assay CVs were 10 and 11% respectively. Androstenedione was assayed using the method of Bosc & Nicolle (1997). The minimal detectable level was 2·5 pg/ tube and the intra-assay CV was 11·6%.…”

Section: Biochemical Characterization Of Follicles (Steroidogenesis Amentioning

confidence: 99%

Anastomosis of the ovarian vein to the hepatic portal vein in sheep induces ovarian hyperstimulation associated with increased LH pulsatility, but only in the absence of the contralateral ovary

Garcés¹,

Mariana²,

Blanc³

et al. 2000

Journal of Endocrinology

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In this study, two experiments were performed, the first of which examined the ovarian response in ewes that were subject to unilateral ovariectomy (ULO) at different intervals (0-14 days) after surgical anastomosis (AN) of the ovarian vein to the mesenteric vein (n=7 ewes), or sham operation (SO; n=4 ewes). Hypertrophy and development of multiple follicular and luteal structures on AN ovaries were observed after ULO, while SO ovaries remained of normal size and appearance after ULO. The second experiment involving 11 ewes (five AN; six SO) aimed to clarify the mechanism by which AN following ULO-induced ovarian hypertrophy and increased follicle development. The results confirmed that there were more large (>5 mm) follicles on AN compared with SO ovaries; however, their rate of atresia was similar. Oestradiol and progesterone concentrations in follicular fluid of class 1 follicles (5-9 mm) were higher in AN ovaries than those in control follicles of the same size collected in the late follicular phase of an induced oestrous cycle. In AN ewes, intrafollicular progesterone concentrations increased while follicular aromatase activity and intrafollicular oestradiol, inhibin A, follistatin and activin A concentrations all decreased as follicle size increased. Oestradiol and progesterone concentrations were substantially higher in ovarian venous blood than in hepatic venous blood, both in AN and SO ewes, whereas inhibin A levels were not significantly modified by passage through the liver in either group. Mean plasma LH concentration, and LH pulse frequency and amplitude increased markedly after AN but were not affected by SO. Plasma FSH showed only a small transient increase after AN, presumably due to the maintenance of inhibin feedback. Injection of prostaglandin F 2 4 days later did not further modify LH or FSH secretion in either group. Full ovariectomy (FO) 9-14 days after AN or SO increased LH secretion markedly in SO ewes but to a lesser degree in AN ewes; FO induced a large and rapid increase in FSH levels in both groups. In conclusion, AN of the ovary to the liver via the mesenteric vein provides a useful model for studying the feedback between the ovary and the hypothalamo-pituitary system and the mechanisms controlling follicle development. The present results indicate that the pattern of LH secretion is an important factor controlling the terminal phase of follicle development in the ewe.

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Steroid productions by Co-cultures of granulosa cells with inner and outer theca cells in preovulatory follicles of gonadotropin stimulated calves

Cited by 6 publications

References 40 publications

Engineered multilayer ovarian tissue that secretes sex steroids and peptide hormones in response to gonadotropins

Engineered multilayer ovarian tissue that secretes sex steroids and peptide hormones in response to gonadotropins

Exploration of the effects of goose TCs on GCs at different follicular stages using a co-culture model

Anastomosis of the ovarian vein to the hepatic portal vein in sheep induces ovarian hyperstimulation associated with increased LH pulsatility, but only in the absence of the contralateral ovary

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