Steroid metabolism in the cat. Biliary and urinary excretion of metabolites of [4-14C]oestradiol

Karim, M. F.; Taylor, William B.

doi:10.1042/bj1170267

Cited by 11 publications

(2 citation statements)

References 9 publications

(14 reference statements)

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“…50 mg of dried feces were extracted with 80% methanol and were analyzed by a 5α-pregnane-3β,11β,21-triol-20-one enzyme immunoassay 15 . FCMs were not analyzed after BDL, because steroid hormones are known to be excreted via the bile duct 72 . Some of the data from the distress assessment was already published in other contexts 16,71,73 .…”

Section: Methodsmentioning

confidence: 99%

MicroRNAs as systemic biomarkers to assess distress in animal models for gastrointestinal diseases

Kumstel

Janssen-Peters

Abdelrahman

et al. 2020

Sci Rep

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Severity assessment of animal experiments is mainly conducted by using subjective parameters. A widely applicable biomarker to assess animal distress could contribute to an objective severity assessment in different animal models. Here, the distress of three murine animal models for gastrointestinal diseases was assessed by multiple behavioral and physiological parameters. To identify possible new biomarkers for distress 750 highly conserved microRNAs were measured in the blood plasma of mice before and after the induction of pancreatitis. Deregulated miRNA candidates were identified and further quantified in additional animal models for pancreatic cancer and cholestasis. MiR-375 and miR-203 were upregulated during pancreatitis and down regulated during cholestasis, whereas miR-132 was upregulated in all models. Correlation between miR-132 and plasma corticosterone concentrations resulted in the highest correlation coefficient, when compared to the analysis of miR-375, miR-203 and miR-30b. These results indicate that miR-132 might function as a general biomarker for distress, whereas the other miRNAs were altered in a disease specific manner. In conclusion, plasma miRNA profiling may help to better characterize the level of distress in mouse models for gastrointestinal diseases.

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Section: Methodsmentioning

confidence: 99%

MicroRNAs as systemic biomarkers to assess distress in animal models for gastrointestinal diseases

Kumstel

Janssen-Peters

Abdelrahman

et al. 2020

Sci Rep

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“…Many hormones, and their metabolites, are excreted in the urine and/or faeces of mammals and the relative concentrations of these excreted substances may be used as an indicator of reproductive condition (Karim & Taylor. 1970;Collins et al, 1979;Hodges, Czekala & Lasley, 1979;Shille et al.…”

Section: Introductionmentioning

confidence: 99%

Non‐invasive monitoring of avian reproduction by simplified faecal steroid analysis

Bishop

Hall

1991

Journal of Zoology

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The measurement of urinary steroid metabolites has proved a reliable method for monitoring reproductive changes in a variety of mammalian species. Development of related techniques for assessing avian reproductive function has shown that sexing of various species is feasible and that the following of individual reproductive changes may be possible. This paper reports on the use of a simplified procedure for assay of specific excreted steroids in a 1: 60 dilution of a 0.4 g dried faecal sample from male and female Japanese quail, and the monitoring of steroid hormone changes in the plasma and faeces throughout a photoperiodically induced reproductive cycle. Immunoreactive steroid measurements were made directly by radioimmunoassay (RIA) of ahquots of faecal homogenates in phosphate buffer and did not involve hydrolysis of steroid conjugates or organic solvent extraction. Measurements were made of immunoreactive testosterone, oestradiol‐3‐glucuronide (E‐3‐gl) and pregnanediol‐3α‐glucuronide (Pd‐3α‐gl). Plasma testosterone and progesterone concentrations, in males and females, were compared to faecal immunoreactive testosterone and Pd‐3α‐gl, respectively. Exposure to stimulatory long daylengths of 20L:4D (LD) resulted in a rise of both plasma and faecal testosterone followed by a reduction after subsequent transfer back to short daylengths of 8L:16D (SD). Male faecal immunoreactive testosterone levels were significantly correlated with the plasma testosterone concentrations (0.59, P<0.001, n= 70) and with the phase of the reproductive cycle. Faecal immunoreactive Pd‐3α‐gl levels increased following exposure to LD, and female values were well correlated with plasma progesterone concentrations (0.79, P <0,001, n= 56) and with the pattern of egg laying. Faecal immunoreactive E‐3‐gl levels were higher in LD than SD birds with higher levels in females compared to males, even under SD photoperiods. For long‐term monitoring of the reproductive status of Japanese quail, faecal immunoreactive testosterone and Pd‐3α‐gl measurements, in a 1:60 dilution of a random 0.4 g dried faecal sample, were found to be as reliable and informative as plasma testosterone and progesterone measurements, respectively.

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Chromatography as a reference technique for the determination of clinically important steroids

Robards

Towers

1990

Biomedical Chromatography

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Chromatographic methods (paper chromatography, thin layer chromatography, high performance liquid chromatography, gas chromatography and gas chromatography-mass spectrometry) for the determination of clinically important steroids in biological specimens are reviewed. The emphasis is on the use of gas chromatography, gas chromatography-mass spectrometry and high performance liquid chromatography as reference rather than routine techniques. Chromatographic methods are compared with colorimetric, fluorimetric and radioimmunoassay procedures in terms of simplicity of operation, cost and ability to analyse large numbers of specimens. The importance of correct specimen collection and storage are discussed. Sample preparation techniques for the various analytical methods are described. These include extraction of free and conjugated steroids from serum, plasma, urine and saliva by solvent partition, with polymer-based resins such as Amberlite XAD-2, DEAE-Sephadex and Sephadex resins bonded with various other function groups and, more recently, with chemically bonded reversed-phase silicas.

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Steroid metabolism in the cat. Biliary and urinary excretion of metabolites of [4-14C]oestradiol

Cited by 11 publications

References 9 publications

MicroRNAs as systemic biomarkers to assess distress in animal models for gastrointestinal diseases

MicroRNAs as systemic biomarkers to assess distress in animal models for gastrointestinal diseases

Non‐invasive monitoring of avian reproduction by simplified faecal steroid analysis

Chromatography as a reference technique for the determination of clinically important steroids

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