Steroid-Induced Cataract: New Perspectives fromIn vitroand Lens Culture Studies

Dickerson, Jaime E.; Dotzel, Eric; Clark, Abbot F.

doi:10.1006/exer.1997.0359

Cited by 70 publications

(52 citation statements)

References 34 publications

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“…Steroids may bind to lens proteins, resulting in the destabilization of the protein structure and the formation of cataract (18) . Systemic methylprednisolone administration has been shown to induce rat lens opacity (19) .…”

Section: Discussionmentioning

confidence: 99%

Oxidative stress of crystalline lens in rat menopausal model

Acer¹,

Pekel²,

Küçükatay³

et al. 2016

Arquivos Brasileiros De Oftalmologia

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Purpose: To evaluate lenticular oxidative stress in rat menopausal models. Methods: Forty Wistar female albino rats were included in this study. A total of thirty rats underwent oophorectomy to generate a menopausal model. Ten rats that did not undergo oophorectomy formed the control group (Group 1). From the rats that underwent oophorectomy, 10 formed the menopause control group (Group 2), 10 were administered a daily injection of methylprednisolone until the end of the study (Group 3), and the remaining 10 rats were administered intraperitoneal streptozocin to induce diabetes mellitus (Group 4). Total oxidant status (TOS), total antioxidant capacity (TAC), and oxidative stress index (OSI) measurements of the crystalline lenses were analyzed. Results: The mean OSI was the lowest in group 1 and highest in group 4. Nevertheless, the difference between the groups was not statistically significant in terms of OSI (p>0.05). The mean TOS values were similar between the groups (p>0.05), whereas the mean TAC of group 1 was significantly higher than that of the other groups (p<0.001). Conclusions:Our results indicate that menopause may not promote cataract formation.

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Section: Discussionmentioning

confidence: 99%

Oxidative stress of crystalline lens in rat menopausal model

Acer¹,

Pekel²,

Küçükatay³

et al. 2016

Arquivos Brasileiros De Oftalmologia

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“…Lenses were graded in the following manner: stage 0, clear; stage 1, hazy cortical region; and stage 2, haziness characterized by a visible demarcation between nuclear and cortical regions. Grading system is based on a modification from Dickerson et al (1997). For multiday exposure experiments, treated medium was exchanged every 24 h for 4 days as indicated.…”

Section: Methodsmentioning

confidence: 99%

“…Stage 0, clear; stage 1, hazy cortical region; stage 2, haziness characterized by a visible demarcation between nuclear and cortical regions. Grading system is based on a modification from Dickerson et al (1997 …”

Section: In Vivo Safety Assessment Of Cig In Rodents Andmentioning

confidence: 99%

Ciglitazone-Induced Lenticular Opacities in Rats: In Vivo and Whole Lens Explant Culture Evaluation

Aleo¹,

Doshna²,

Navetta³

2004

J Pharmacol Exp Ther

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The cataractogenic potential of the thiazolidinedione ciglitazone (CIG) was investigated in vivo and in vitro. In the rat, CIG caused a dose-dependent (30 -300 mg/kg/day) increase in incidence and severity of nuclear cataract formation during a 3-month nonclinical safety assessment study. Potential mechanisms of toxicity were surveyed using whole rat lens explants exposed to CIG with or without various inhibitors of cataract formation. In vitro, CIG caused a concentration-(0.375-30 M) and time-dependent (3-24 h) change in biochemical [ATP content or mitochondrial reduction of the tetrazolium dye 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) and reduced glutathione (GSH) content] and morphometric (lens wet weight and clarity) markers of damage. Within 3 h of exposure, 7.5 M CIG decreased lens ATP content 37 Ϯ 7% (percentage of difference from control, p Ͻ 0.05). After 24 h of exposure, lens ATP content, MTT reduction, and GSH content declined 57 Ϯ 5, 30 Ϯ 28, and 42 Ϯ 8%, respectively. Lens wet weight increased 17 Ϯ 4% with a concomitant decrement in lens clarity. Pretreating lenses with the mitochondrial calcium uniport inhibitor ruthenium red (RR) partially or fully protected lenses from toxicity. In contrast, the antioxidant dithiothreitol, aldose reductase inhibitor sorbinil, and selective cell-permeable calpain inhibitors [calpain II inhibitor and (2S,3S)-trans-epoxysuccinyl-L-leucylamido-3-methylbutane ethyl ester (E64d)] were ineffective in providing protection under the present testing conditions. Early and selective changes in lenticular ATP content and the partial or full protective effect of RR suggest that alterations in lens bioenergetics may play an important role in CIG-induced cataract formation. Lens explant cultures were successfully used to select two thiazolidinediones that lacked cataractogenic activity when evaluated in 3-month rat safety assessment studies.

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“…[3][4][5][6][7][8][9][10][11][12][13][14][15][16][17] Among these GSTs, the majority (GSTA, GSTM, GSTP, GSTT, GSTZ and GSTO) are located in the cytosolic fraction and GSTK is located in the mitochondrial fraction. Based on the amino acid sequences, cytosolic GSTs within α class exhibit a greater than 40% identity and those between classes share an identity of less than 25%.…”

Section: Human Glutathione S-transferasesmentioning

confidence: 99%

“…Smoking is just one of the many established or putative risk factors for cataracts, which also include advanced age, trauma, persistent intraocular inflammation, ultraviolet radiation, ionizing radiation, diabetes mellitus, hypoparathyroidism, prolonged corticosteroid administration, aromatic hydrocarbon intake and high body mass index. [1][2][3][4][5][6] In cataracts associated with smoking, naphthalene is reported to be a risk factor. [2][3][4][5][6] Under naphthalene exposure, it has been suggested that cataract formation follows a similar process to the one seen in the development of senile cataracts, 7) suggesting that studies of the mechanisms of naphthalene-cataracts could help lead to a prevention of cataracts in- The polymorphic expression of glutathione S-transferase (GST) in animal lenses has been demonstrated by purification with affinity chromatography, immunochemical analysis with Western-blotting and amino acid sequencing of the N-terminal regions.…”

Section: Introductionmentioning

confidence: 99%

Role of Glutathione S-Transferases in Lens under Oxidative Stress

Terada

2005

JOURNAL OF HEALTH SCIENCE

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Steroid-Induced Cataract: New Perspectives fromIn vitroand Lens Culture Studies

Cited by 70 publications

References 34 publications

Oxidative stress of crystalline lens in rat menopausal model

Oxidative stress of crystalline lens in rat menopausal model

Ciglitazone-Induced Lenticular Opacities in Rats: In Vivo and Whole Lens Explant Culture Evaluation

Role of Glutathione S-Transferases in Lens under Oxidative Stress

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