Steroid Hormones and Prolactin in Porcine Follicular Fluid in Estrous Cycle and Early Pregnancy

Przala, J; A, Grazul; Więsak, Teresa; Muszyńska, Anna; Dusza, Luiza

doi:10.1055/s-0029-1210499

Cited by 9 publications

(6 citation statements)

References 7 publications

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“…For Landrace gilts, these concentrations were also in the range as reported in the literature (Ainsworth et al. 1980; Przała et al. 1985; Blödow et al.…”

Section: Discussionsupporting

confidence: 79%

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Superovulatory Ovarian Response in Mangalica Gilts is Not Influenced by Feeding Level

Egerszegi

Hazeleger

Rátky

et al. 2007

Reprod Domestic Animals

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The aim of the study was to compare how different feeding levels affect the ovarian potential of follicular development and oocyte maturation in response to superovulatory treatment in native Mangalica (M, n = 17) compared with Landrace (L, n = 20) pigs. Gilts of both breeds were fed high-energy (HI-2.5 kg) or low-energy (LO - 1.25 kg) feed during oestrus synchronization (15 days of Regumate feeding) till the time of oocyte aspiration (Day 6 after Regumate). Follicular growth was stimulated by the administration of 1000 IU equiue choriou gonadotropiu (eCG) 24 h after Regumate treatment, and ovulation was induced by injection of 750 IU human choriou gonadotropiu (hCG) 80 h after eCG administration. Ultrasound (US) investigation was done three times (4-10 h before, and 40-44 and 72-74 h after eCG administration) for the observation of follicular development. Oocyte and follicular fluid (FF) were collected endoscopically 34 h after hCG injection. Cumulus-oocyte complexes were evaluated, their morphology determined, and thereafter fixed and stained for chromatin evaluation. Oocytes were classified as meiosis-resumed (germinal vesicle breakdown, diakinesis, metaphase I to anaphase I) or matured (telophase I and metaphase II). FF concentrations of oestradiol and progesterone were measured by validated radioimmunoassays. In L gilts, differences were observed between HI and LO in the number of preovulatory follicles (32.3 +/- 10.5 vs 17.1 +/- 12.3, p < 0.05), but not in M (25.3 +/- 2.9 vs 28.8 +/- 7.3, p > 0.05). Initial follicular growth was not affected by feeding levels; however, preovulatory follicle size was larger in M (7.1 +/- 0.9 and 6.9 +/- 1.1 mm vs 5.7 +/- 0.7 and 5.5 +/- 0.8 mm; p < 0.05). No differences were obtained with relation to mature chromatin configuration in both breeds (L gilts: HI - 70% and LO-67% vs M gilts: HI - 67% and LO - 63%). A twofold higher oestradiol concentration was detected in FF of HI-M and LO-M (29.6 +/- 6.8 and 30.9 +/- 10.3 ng/ml respectively) compared with that of L (16.9 +/- 9.7 and 17.9 +/- 3.6 ng/ml, respectively; p < 0.05). The mean FF progesterone level was nearly fivefold higher in M (2020.4 +/- 1056 and 1512.2 +/- 1121.8 ng/ml) compared with L (386.2 +/- 113.7 and 298.8 +/- 125.9 ng/ml, p < 0.05). The results indicate an influence of the feeding of altered energy on the number of recruitable preovulatory follicles in modern Landrace but not in native Mangalica breed. Moreover, the follicular steroid hormone milieu differs between Landrace and Mangalica gilts but not depending on feeding levels. Oocyte maturation was not affected by diet.

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“…For Landrace gilts, these concentrations were also in the range as reported in the literature (Ainsworth et al. 1980; Przała et al. 1985; Blödow et al.…”

Section: Discussionsupporting

confidence: 79%

“…Although FF concentrations of E 2 were significantly higher in Mangalica gilts, they were in the preovulatory range reported elsewhere for other breeds (Ainsworth et al. 1980; Przała et al. 1985; Blödow et al.…”

Section: Discussionsupporting

confidence: 44%

Superovulatory Ovarian Response in Mangalica Gilts is Not Influenced by Feeding Level

Egerszegi

Hazeleger

Rátky

et al. 2007

Reprod Domestic Animals

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“…PRL is present in the FF of the human [7], porcine [27] and bovine [28] ovarian follicle and it can originate locally, since the PRL mRNA is expressed in human ovarian follicular cells [29] and PRL is present in granulosa cells of mature rat follicles [30]. The PRL levels measured in bovine FF were within the range reported for bovines (20–80 ng/ml) [31] and humans (30–137 ng/ml) [32, 33].…”

Section: Discussionmentioning

confidence: 99%

Prolactin in Ovarian Follicular Fluid Stimulates Endothelial Cell Proliferation

Castilla

García²,

Cruz³

et al. 2009

J Vasc Res

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Angiogenesis is essential for the growth and maturation of the ovarian follicle and its transition into the corpus luteum. In addition to the main proangiogenic factors, vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF), follicular fluid (FF) contains the hormone prolactin (PRL), which is known to promote angiogenesis in vivo. Here, we show that FF from large follicles, which contains twice the PRL level of FF from small follicles, stimulates endothelial cell proliferation to a greater extent than the latter, and that immunoneutralization of PRL prevents FF from stimulating endothelial cell proliferation. Notably, the FF increases the expression of the short and long PRL receptor isoforms in endothelial cells, and a purified PRL standard stimulates endothelial cell proliferation but only after the cells have been pretreated with FF. However, purified PRL activates the JAK2/STAT3 pathway in endothelial cells in the absence of pretreatment with FF. In summary, PRL present in the FF stimulates the proliferation of endothelial cells. This effect likely involves the upregulation of the short and long PRL receptor isoforms and is independent of PRL-induced JAK2/STAT3 signaling.

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“…During estrous cycle, estrogen is synthesized by the growing follicles and later, after ovulation, progesterone is synthesized and secreted by the corpus luteum and by the placenta after placentation (Ash and Heap, 1975;Flowers et al, 1991;Przała et al, 1985). These hormones initiated the histological changes in the uterus in preparation for implantation and the growth and development of uterus and embryo in the beginning of pregnancy followed by the growth and development of fetus and the placenta until parturition (Gray et al, 2001;Bazer, 2004, 2002;.…”

Section: Introductionmentioning

confidence: 99%

Production of Superior Pigs by Injecting the Sows with Gonadotropin Prior to Mating

Rayer

Maheswari²,

Muladno³

et al. 2015

anprod

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Abstract. An experiment was conducted to produce superior pigs with improved growth phenotypes and survival during postnatal growth by injecting the sows with gonadotropin prior to mating. The experiment consisted of 2 stages. In the first stage, 12 sows were divided into 2 groups i.e., sows injected with NaCl0.95% as a control (NSO) and sows injected with PG600 (SO) prior to mating. Parameters measured were growth phenotypes and survival at birth and during pre-weaning period. In the second stage, 24 of weaned pigs (age 8 weeks) from the first stage were selected (6 males and 6 females from NSO group and 6 males and 6 females from SO group) to be used for measurement of growth performance. The experimental pigs were raised and observed until the age of 28 weeks (7 month). The body weights were measured monthly. The results of the experiment showed that improvement of uterine environment by gonadotropin injection of the sows prior to mating dramatically improved birth weight with a very homogenous birth weight within litter size. Improved birth weight and within-litter variation of birth weight improved pre-weaning growth performance and survival that finally dramatically increased weaning weight and total weight of weaned pigs per sow. After weaning, pigs born to SO sows grew faster and had around 10 kg higher body weight as compared to control at the age of 7 month. It was concluded that superior pigs could be produced by improving endogenous secretion of pregnant hormone during pregnancy.Key words: Birth weight, growth phenotypes, survival, prenatal growth, postnatal growth Abstrak. Penelitian ini dilakukan untuk menghasilkan anak-anak babi unggul dengan fenotipe pertumbuhan dan daya hidup yang lebih baik selama pertumbuhan pascalahir dengan cara menyuntik induk babi dengan gonadotropin sebelum pengawinan. Penelitian terdiri atas dua tahapan. Penelitian tahap pertama, 12 ekor induk babi dikelompokkan ke dalam 2 kelompok, 1) kelompok kontrol, yaitu induk yang disuntik dengan NaCl 0.95% (NSO) dan 2) kelompok yang disuntik dengan PG600 sebelum pengawinan (SO). Parameter yang diukur ialah fenotipe pertumbuhan dan daya hidup anak pada saat lahir dan selama periode prasapih. Tahap kedua, 24 anak babi lepas sapih dari percobaan tahap pertama (umur 8 minggu) dipilih (6 jantan dan 6 betinadari kelompok NSOdan 6 jantan dan 6 betina dari kelompok SO) untuk digunakan dalam pengukuran kinerja pertumbuhan pascasapih. Babi percobaan dibesarkan dan diamati sampai umur 28 minggu (7 bulan). Bobot badan diukur setiap bulan. Hasil penelitian menunjukkan bahwa perbaikan lingkungan uterus dengan cara penyuntikan induk dengan gonadotropin sebelum pengawinan secara dramatis memperbaiki bobot lahir anak dengan bobot lahir per induk yang lebih seragam. Perbaikan bobot lahir dan keseragaman bobot lahir memperbaiki kinerja pertumbuhan prasapih dan kelangsungan hidup anak yang pada akhirnya secara dramatis meningkatkan bobot sapih dan total bobot anak yang disapih per ekor induk. Setelah penyapihan, anak babi yang dilahirkan oleh induk y...

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Steroid Hormones and Prolactin in Porcine Follicular Fluid in Estrous Cycle and Early Pregnancy

Cited by 9 publications

References 7 publications

Superovulatory Ovarian Response in Mangalica Gilts is Not Influenced by Feeding Level

Superovulatory Ovarian Response in Mangalica Gilts is Not Influenced by Feeding Level

Prolactin in Ovarian Follicular Fluid Stimulates Endothelial Cell Proliferation

Production of Superior Pigs by Injecting the Sows with Gonadotropin Prior to Mating

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