Steroid hormone receptor status and prognosis in breast tumours of different racial groups

Pegoraro, R.J.; Nirmul, D.; Joubert, S. M.

doi:10.1016/0022-4731(87)91144-7

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“…The frequency of ER expression is known to vary with menstrual status and age (Thorpe et al, 1987) as well as with ethnic origin (Levin et al, 1978;Pegoraro et al, 1986) and is, moreover, an indicator of disease biology (Maynard et al, 1978;Clark et al, 1984). In this regard, it should be noted that the mean age of the patient included in this study was 52 years, 43% were premenopausal, 60% were black and 40% presented with stage 4 disease.…”

Section: Discussionmentioning

confidence: 99%

Comparison between immunocytochemical and polymerase chain reaction techniques for detection of oestrogen receptor and transforming growth factor β in breast cancer

Amoils

Seymour²,

Wr³

1996

Br J Cancer

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Summary The utility of the polymerase chain reaction (PCR) as a technique for determining the expression of transforming growth factor ,B (TGF-fl) and of the oestrogen receptor (ER) in clinical breast cancer tissue was examined. PCR analysis was compared with immunocytochemical assays for TGF-,B and for ER. Seventy confirmed breast carcinoma samples were analysed for ER using both techniques with a statistically highly significant concordance (P<0.001) between the two methods. Nineteen samples were observed to be ER positive and 46 samples were found to be ER negative by both techniques. Forty-eight samples were analysed for TGF-,B using both PCR and immunocytochemistry. Of the 24 samples observed to be positive for TGF-,B by immunocytochemistry, all were found to be positive for TGF-,B mRNA (PCR). Similarly, the 24 samples observed to be TGF-,B negative by immunocytochemistry were also negative for TGF-fl mRNA, indicating 100% specificity and 100% sensitivity of the PCR technique. PCR is therefore considered a viable technique for analysis of both ER and TGF-,B in small samples such as fine-needle aspirates.

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Section: Discussionmentioning

confidence: 99%