Steroid Hormone Analysis by Tandem Mass Spectrometry

Abstract

BACKGROUND New high-performance liquid chromatography/ tandem mass spectrometry (LC-MS/MS) methods are among the most successful approaches to improve specificity problems inherent in many immunoassays. CONTENT We emphasize problems with immunoassays for the measurement of steroids and review the emerging role of LC-MS/MS in the measurement of clinically relevant steroids. The latest generation of tandem mass spectrometers has superior limits of quantification, permitting omission of previously employed derivatization steps. The measurement of steroid profiles in the diagnosis and treatment of congenital adrenal hyperplasia, adrenal insufficiency, chronic pelvic pain and prostatitis, oncology (breast cancer), and athletes has important new applications. CONCLUSIONS LC-MS/MS now affords the specificity, imprecision, and limits of quantification necessary for the reliable measurement of steroids in human fluids, enhancing diagnostic capabilities, particularly when steroid profiles are available.

A comparison of salivary testosterone measurement using immunoassays and tandem mass spectrometry

“…The use of LC-MS/MS as an analytical tool has become popular in a growing number of laboratories seeking to quantify hormone concentrations (Soldin & Soldin, 2009;Field, 2013;Keefe et al, 2014). The technique demonstrates impressive analytical specificity and is argued to be free of many of the limitations of IAs (e.g.…”

“…Based on the increasing use of LC-MS/MS for measuring hormones (for reviews see Soldin &Soldin, 2009 andField, 2013), researchers may wish to adopt LC-MS/MS for more accurate testosterone measurement, when feasible. LC-MS/MS is more costly than conducting…”

Analytical Performance of LC-MS/MS Method for Simultaneous Determination of Five Steroids in Serum

“…Novel mass spectrometry techniques hyphened with liquid chromatography has become an important tool that continues to accelerate clinical research and clinical laboratories routines since the past decade [11]. The last generation of tandem mass spectrometers has superior limits of quantification, permitting omission or previously employed derivatization steps [11].…”

Az ösztrogénmetabolom biológiai és klinikai jelentősége lokális folyamatokban

“…Általános vélekedéssé vált azonban, hogy ezek analitikai teljesítményjellemzői sok tekintetben elfogadhatatlanok. A kialakult álláspontot igazolja, hogy a College of American Pathologists által 2008-ban lebonyolított szteroid külső körvizsgálatban azonos immunoassay-módszerrel kapott E2-eredmények között kilencszeres eltérés volt [62]. Hsing és mtsai radioimmunoassay (RIA) és gázkromatográffal kapcsolt tömeg-spektrométerrel (GC-MS), illetve folyadékkromatográf-fal kapcsolt tandem tömegspektrométerrel (LC-MS/ MS) végzett vizsgálatok során kapott eredményeket hasonlítottak össze, és azt találták, hogy az ösztrogének ÖSSZEFOGLALÓ KÖZLEMÉNY RIA-módszerrel kapott szintjei mintegy 50%-kal voltak magasabbak [63].…”