Steroid Assays in Paediatric Endocrinology

Honour, John W.

doi:10.4274/jcrpe.v2i1.1

Cited by 26 publications

(17 citation statements)

References 186 publications

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“…It is crucial to note that the measurement of hormone concentrations, especially in children, itself is the object of current research Granger et al (2004). point out that the manner of the collection and storage of saliva samples may affect results Honour (2010). investigated steroid assays in pediatric endocrinology and concluded that in this research area, the discussion of unusual results, and a possible improvement of test results via the usage of more labor-intensive methods such as chromatography or (tandem) mass spectrometry might be of importance.Developmental PsychobiologyMental Rotation and Salivary Hormones in (Pre-)Puberty…”

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confidence: 99%

Is there a relationship between the performance in a chronometric mental‐rotations test and salivary testosterone and estradiol levels in children aged 9–14 years?

Quaiser-Pohl

Jansen

Lehmann

et al. 2015

Developmental Psychobiology

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The consistent gender differences favoring males in some spatial abilities like mental rotation have raised the question of whether testosterone or other gonadal hormones contribute to these differences--especially because such gender differences seem to appear mainly from the age of puberty on. Studies generally suggest that spatial ability is facilitated by moderately high testosterone levels (i.e., levels that are relatively high for females and relatively low for males). However, the role of sex steroids for mental-rotation performance of (pre-) pubertal children has not been the focus of research, yet. In our study, the relationships between different aspects of mental-rotation performance (accuracy, reaction time, rotation speed) and salivary testosterone and estradiol levels were investigated. Subjects were 109 children (51 boys and 58 girls) aged between 9 and 14 years (M = 11.41, SD = 1.74). They performed a chronometric mental-rotations test, in which the stimuli consisted of three-dimensional drawings of Shepard and Metzler cube figures. In addition, saliva samples were gathered for the analysis of free testosterone and estradiol levels. Results showed a significant gender difference in reaction time and rotational speed in favor of boys, and a significant age, but no gender difference in testosterone and estradiol levels. We found no significant relationships between hormonal levels and any measure of mental-rotation performance.

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mentioning

confidence: 99%

Is there a relationship between the performance in a chronometric mental‐rotations test and salivary testosterone and estradiol levels in children aged 9–14 years?

Quaiser-Pohl

Jansen

Lehmann

et al. 2015

Developmental Psychobiology

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“…It has generally been proposed that the presence of interference peaks co-existing in biological matrixes and co-eluting with the analyte of interest caused ionization suppression, reducing the detection sensitivity (12,13). For the measurement of steroids in organs, LC-MS analysis has several advantages over immunoassays, including a better specificity and the ability to quantify numerous steroids in a single run (14,15). Especially, liquid chromatographyelectrospray ionization time-of-flight mass spectrometry (LC-TOFMS) made possible to perform the accurate mass determination of components containing matrixes.…”

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confidence: 99%

Accurate determination of tissue steroid hormones, precursors and conjugates in adult male rat

Maeda

Tanaka

Suzuki

et al. 2012

The Journal of Biochemistry

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The actual levels of steroid hormones in organs are vital for endocrine, reproductive and neuronal health and disorders. We developed an accurate method to determine the levels of steroid hormones and steroid conjugates in various organs by an efficient preparation using a solid-phase-extraction cartridge. Each steroid was identified by the precursor ion spectra using liquid chromatographyelectrospray ionization timeof-flight mass spectrometry, and the respective steroids were quantitatively analysed in the selected reaction monitoring mode by liquid chromatograph-mass spectrometry/mass spectrometry (LC-MS/MS). The data showed that significant levels of testosterone, corticosterone and precursors of both hormones were detected in all organs except liver. The glucuronide conjugates of steroid hormones and the precursors were detected in all organs except liver, but sulfate conjugates of these steroids were observed only in the target organs of the hormones and kidney. Interestingly, these steroids and the conjugates were not observed in the liver except pregnenolone. In conclusion, an accurate determination of tissue steroids was developed using LC-MS analysis. Biosynthesis of steroid hormones from the precursors was estimated even in the target organs, and the delivery of these steroid conjugates was also suggested via the circulation without any significant hepatic participation.

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“…The highest urinary excretion rate of cortisol metabolites is between 1000 h and 1800 h. 51 There is an increase in androgen sulphate excretion and other major androgen metabolites in premature adrenarche, whereas very distinctive profiles are seen in adrenocortical tumours. 21-hydroxylase deficiency, the commonest form of CAH, shows an increase in 17-OHP and 21-deoxycortisol metabolites with low cortisol and corticosterone metabolites.…”

Section: Tests Of Adrenal Functionmentioning

confidence: 99%

The investigation of children and adolescents with abnormalities of pubertal timing

et al. 2016

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Concerns with pubertal development are common and can cause considerable distress to patients and their carers. Many presentations reflect normal variations of pubertal timing and primarily require reassurance, although patients may opt for interventions. Other presentations need active management to avoid significant adverse effects on growth and psychosocial development. All should undergo careful assessment, particularly as some children or adolescents presenting with abnormalities in pubertal timing may have serious pathology which requires urgent investigations and treatment. This review describes the appropriate investigations and their interpretation for young people presenting with disorders in pubertal timing.

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Steroid Assays in Paediatric Endocrinology

Cited by 26 publications

References 186 publications

Is there a relationship between the performance in a chronometric mental‐rotations test and salivary testosterone and estradiol levels in children aged 9–14 years?

Is there a relationship between the performance in a chronometric mental‐rotations test and salivary testosterone and estradiol levels in children aged 9–14 years?

Accurate determination of tissue steroid hormones, precursors and conjugates in adult male rat

The investigation of children and adolescents with abnormalities of pubertal timing

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