Z Allg Mikrobiol

1975

DOI: 10.1002/jobm.3630150711

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Steriodumwandelnde Enzyme aus Mikroorganismen. I. Herstellung und Reinigung einer Δ4-3-Ketosteroid-5α-reduktase ausMycobacterium smegmatis

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Steroidumwandelnde Enzyme aus Mikroorganismen X. Anreicherung einer 4-En-3-oxosteroid-5α-Reduktase ausMycobacterium smegmatis sowie Abtrennung und Anreicherung des Apoenzyms mit Hilfe der Affinitätschromatographie

et al. 1979

Z Allg Mikrobiol

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The 4-en-3-oxosteroid-5 alpha-reductase from Mycobacterium smegmatis was bound biospecifically on the affinant containing an immobilized testosterone ligand. The enzyme obtained by elution with ethylene glycol and urea in a 32 fold purity has a S. A. of 8.73 X 10(-3) microM androstenedione min-1 mg-1. The coenzyme (FAD) could be separated from the immobilized enzyme substrate complex on the affinity matrix, in the presence of (NH4)2SO4 at pH 3.0. After elution of the apoenzyme 97% of the initial enzyme activity was obtained by incubation with FAD. The reactivated enzyme results in a 40-fold enrichment.

Steroidumwandelnde Enzyme aus Mikroorganismen X. Anreicherung einer 4-En-3-oxosteroid-5α-Reduktase ausMycobacterium smegmatis sowie Abtrennung und Anreicherung des Apoenzyms mit Hilfe der Affinitätschromatographie

et al. 1979

Z Allg Mikrobiol

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The 4-en-3-oxosteroid-5 alpha-reductase from Mycobacterium smegmatis was bound biospecifically on the affinant containing an immobilized testosterone ligand. The enzyme obtained by elution with ethylene glycol and urea in a 32 fold purity has a S. A. of 8.73 X 10(-3) microM androstenedione min-1 mg-1. The coenzyme (FAD) could be separated from the immobilized enzyme substrate complex on the affinity matrix, in the presence of (NH4)2SO4 at pH 3.0. After elution of the apoenzyme 97% of the initial enzyme activity was obtained by incubation with FAD. The reactivated enzyme results in a 40-fold enrichment.

Steroid transforming enzymes from microorganisms IV. Purification and cofactor requirement of the 4-ene-3-oxosteroid-5α-reductase from Mycobacterium smegmatis

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et al. 1977

Journal of Steroid Biochemistry

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Steroid transforming enzymes from microorganisms—III: Properties of 4-ene-3-oxosteroid-5α-reductase from Mycobacterium smegmatis

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et al. 1977

Journal of Steroid Biochemistry

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