Sterilizing Effect of Ozone on Live Spores of Anthrax Bacillus

Abstract

flux, passing out the chamber 2. After the destructor the output flux was sent to an exhaust hood. Results of experimental research of ozone-oxygen Live spores of the anthrax bacillus -dry live STI effect on the most stable known spore-forming bacteria -vaccine ofthe anthrax bacillus was used as a test-microbe. spores of anthrax bacillus are presented. Sterilizing effect Live spores of Bacillus Stearothermophilus, used for of the ozone effect at concentration of 20 mg/l and autoclave operation control, were used as the reference processing time of 60 min is detected.culture. Initial cultures were dissolved with sterile physiological solution pH=7.2 at room temperature. Four samples of the

The use of ozone gas for the inactivation of Bacillus anthracis and Bacillus subtilis spores on building materials

“…Buranov et al [22] in a conference proceeding reported that decontamination efficacy > 6 LR with an avirulent strain of B. anthracis was achieved with ozone concentrations of1 0,000 ppm, consistent with the present study. Currier et al [13] demonstrated � 6 LR against spores of B. globigii on glass coupons when using 9,000 ppm ozone with only a 1 h contact time.…”

“…Additionally, there is a paucity of data for the use of ozone gas in the inactivation of B. anthracis spores on surfaces. In a short conference proceeding, Buranov et al [22] indicated that 20 mg/L (~10,000 ppm) ozone for 1 h was sufficient for complete inactivation of 10 6 and 10 8 spores of a B. anthracis vaccine strain deposited on an empty Petri plate; however, minimal data and details on the methods used were provided.…”