Sterilization effects on ultrathin film polymer coatings for silicon‐based implantable medical devices

Iqbal, Zohora; Moses, Willieford; Kim, Steven; Kim, Eun Jung; Fissell, William H.; Roy, Shuvo

doi:10.1002/jbm.b.34039

Cited by 14 publications

(11 citation statements)

References 43 publications

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“…where L (length of pore, 2.325 mm), h (height of pore, 400 nm), n (fixed number of pores on the test substrate, 1.56 Â 10 7 pores), and l (viscosity of water) are fixed, DP (transmembrane pressure) is varied, and Q (volumetric flow rate) is measured. 13,17,56 In vitro protein adsorption (fibrinogen and albumin)…”

Section: Surface Coating Characterizationmentioning

confidence: 99%

“…Protein adsorption on the surfaces of the substrates was determined by conducting enzyme-linked immunosorbent assay (ELISA) following protocol published previously. 17,44 Substrates were placed in 24-well tissue culture polystyrene (TCPS) plates and incubated with D-PBS for 1.5 h. D-PBS was replaced with 0.5 mL of single protein solution: 1 mg/mL concentration of human fibrinogen (F3879, Sigma-Aldrich) or human albumin (AB19183, Abcam, Cambridge, MA, USA) was added and allowed to incubate at 37 C for 1.5 h. All substrates were rinsed five times with D-PBS. Surfaces were then blocked using bovine serum albumin (A7906, BSA, !98%, Sigma-Aldrich) using 1 mg/mL BSA solution for 1.5 h. The substrates were rinsed five times with D-PBS and transferred to a new 24-well TCPS well.…”

Section: Surface Coating Characterizationmentioning

confidence: 99%

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In vitro and in vivo hemocompatibility assessment of ultrathin sulfobetaine polymer coatings for silicon-based implants

et al. 2019

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Highly uniform silicon nanopore membranes were developed for applications in implantable bioartificial organs. A robust, readily scalable, non-fouling surface coating is required to enhance silicon nanopore membrane hemocompatibility. However, the coating must be ultrathin to keep the nanopores from occluding. Recently, zwitterionic brush polymers have demonstrated significantly lower fouling under biological conditions. In this study, we explore ultrathin zwitterionic poly(sulfobetaine methacrylate) (pSBMA) surface coating at sub-5 nm thickness. Membrane hydraulic permeability was measured before and after surface modification of silicon nanopore membranes, and pores were found to be patent and in agreement with coating thickness measurements. Coating stability was analyzed under biological shear as well as under blood flow in vitro and in vivo. Following exposure to shear over 24 h, coatings were characterized via X-ray photoelectron spectroscopy, goniometry, and ellipsometry, and found to survive biological shear. In vitro blood experiments with fresh human blood as well as in vivo 7-day and 26-day implants in a porcine model demonstrate minimal platelet adhesion and activation with pSBMA surface modification compared to unmodified silicon exposed to fresh human blood in vitro. These results demonstrate that ultrathin pSBMA surface modification is a viable choice for application in blood contacting implants with critical nanoscale features.

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Section: Surface Coating Characterizationmentioning

confidence: 99%

Section: Surface Coating Characterizationmentioning

confidence: 99%

In vitro and in vivo hemocompatibility assessment of ultrathin sulfobetaine polymer coatings for silicon-based implants

et al. 2019

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“…Indeed, the recent increase in exploration of SB-based materials for in vivo evaluations brings their clinical use closer to reality. 213,214 Beyond surface uses, there are a variety of promising directions for SB-based zwitterion biomaterials in applications such as wound healing or insulin administration, which may be ripe for translation in future years. 136,215 Many of the possible applications for which the antifouling properties of SB would prove beneficial remain to be explored.…”

Section: Discussionmentioning

confidence: 99%

Biomedical Uses of Sulfobetaine-Based Zwitterionic Materials

2020

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Protein fouling can render a biomedical device dysfunctional, and also serves to nucleate the foreign body reaction to an implanted material. Hydrophilic coatings have emerged as a commonly applied route to combat interface-mediated complications and promote device longevity and limited inflammatory response. While polyethylene glycol has received a majority of the attention in this regard, coatings based on zwitterionic moieties have been more recently explored. Sulfobetaines in particular constitute one such class of zwitterions explored for use in mitigating surface fouling, and have been shown to reduce protein adsorption, limit cellular adhesion, and promote increased functional lifetimes and limited inflammatory responses when applied to implanted materials and devices. Here, we present a focused review of the literature surrounding sulfobetaine, beginning with an understanding of its chemistry and the methods by which it is applied to the surface of a biomedical device in molecular and polymeric forms, and then advancing to the many early demonstrations of function in a variety of biomedical applications. Finally, we provide some insights into the benefits and challenges presented by its use, as well as some outlook on the future prospects for using this material to improve biomedical device practice by addressing interface-mediated complications.

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“…Although the hydration layer influences the water structure by ions in the aqueous medium, protein molecules attached at the interface do not influence the hydration structure. , These findings are important to explain low-protein-adsorption phenomena on these zwitterionic polymers. Biological responses, such as platelet adhesion and bacteria adhesion, following the protein adsorption process on these polymers, are also suppressed, when they are examined in vitro and ex vivo . ,,,,− For these polymers to be used as materials for preparing the medical devices, it is necessary to exhibit not only biological performance of the polymers but also their safety and stability for sterilization, , long-term storage, and clinical use. The number of articles concerning these polymers increases year by year, and it is expected that biomedical devices incorporating them will soon be developed in a similar manner to that found with MPC polymers.…”

Section: Concluding Remarks and Future Perspectivementioning

confidence: 99%

“…responses, such as platelet adhesion and bacteria adhesion, following the protein adsorption process on these polymers, are also suppressed, when they are examined in vitro and ex vivo. 111,112,115,116,127−129 For these polymers to be used as materials for preparing the medical devices, it is necessary to exhibit not only biological performance of the polymers but also their safety and stability for sterilization, 130,131 long-term storage, and clinical use. The number of articles concerning these polymers increases year by year, and it is expected that biomedical devices incorporating them will soon be developed in a similar manner to that found with MPC polymers.…”

Section: ■ Cardiovascular Applicationsmentioning

confidence: 99%

Blood-Compatible Surfaces with Phosphorylcholine-Based Polymers for Cardiovascular Medical Devices

2018

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For the acquisition of blood-compatible materials, various hydrophilic polymers for surface modification have been examined. Among them, polymers with a representative phospholipid polar group, the phosphorylcholine (PC) group, are a successful example. These polymers were designed from inspiration of the cell membrane surface and provide protein adsorption resistance even following contact with plasma. This important property is based on the unique hydration state of water molecules surrounding hydrated polymer; in other words, water molecules weakly interact with the polymers and maintain their favorable cluster structure through hydrogen bonding. These polymers are not only hydrophilic, but also electrically neutral, important characteristics which make hydrogen bonding with water molecules less likely to occur and avoid hydrophobic interactions. Phosphorylcholine groups and other zwitterionic structures are significant as hydrophilic functional groups meeting these important requirements. In this review, blood compatibility of a polymer having a PC group is introduced in relation to its hydration structure, followed by a description of the applications of this polymer to cardiovascular medical devices.

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Sterilization effects on ultrathin film polymer coatings for silicon‐based implantable medical devices

Cited by 14 publications

References 43 publications

In vitro and in vivo hemocompatibility assessment of ultrathin sulfobetaine polymer coatings for silicon-based implants

In vitro and in vivo hemocompatibility assessment of ultrathin sulfobetaine polymer coatings for silicon-based implants

Biomedical Uses of Sulfobetaine-Based Zwitterionic Materials

Blood-Compatible Surfaces with Phosphorylcholine-Based Polymers for Cardiovascular Medical Devices

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