Sterilization effects of UV laser irradiation on<i>Bacillus atrophaeus</i>spore viability, structure, and proteins

Nguyen, My-Chi Thi; Nguyen, Huu‐Quang; Jang, Hanbyeol; Noh, Sojung; Lee, Seong-Yeon; Jang, Kyoung‐Soon; Lee, Jaebeom; Sohn, Youngku; Yee, Ki‐Ju; Jung, Heesoo; Kim, Jeongkwon

doi:10.1039/d1an01717a

Cited by 8 publications

(9 citation statements)

References 64 publications

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“…The sodium hypochlorite solution was purchased from Shanghai Aladdin Biochemical Science and Technology Co. Ltd. (product no. S298682), with a density of 1.2 g/cm 3 and an effective chlorine content of 100-140 g/l. Anhydrous sodium thiosulphate was also purchased from Shanghai Aladdin Biochemical Science and Technology Co. Ltd. (product no.…”

Section: Measurement Of Sodium Hypochlorite Treatment and Internal Mo...mentioning

confidence: 99%

Single‐cell analysis reveals microbial spore responses to sodium hypochlorite

Yang,

Yuan,

et al. 2024

Journal of Biophotonics

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Pollution from toxic spores has caused us a lot of problems because spores are extremely resistant and can survive most disinfectants. Therefore, the detection of spore response to disinfectant is of great significance for the development of effective decontamination strategies. In this work, we investigated the effect of 0.5% sodium hypochlorite on the molecular and morphological properties of single spores of Bacillus subtilis using single‐cell techniques. Laser tweezers Raman spectroscopy showed that sodium hypochlorite resulted in Ca2+‐dipicolinic acid release and nucleic acid denaturation. Atomic force microscopy showed that the surface of treated spores changed from rough to smooth, protein shells were degraded at 10 min, and the permeability barrier was destroyed at 15 min. The spore volume decreased gradually over time. Live‐cell imaging showed that the germination and growth rates decreased with increasing treatment time. These results provide new insight into the response of spores to sodium hypochlorite.

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Section: Measurement Of Sodium Hypochlorite Treatment and Internal Mo...mentioning

confidence: 99%

Single‐cell analysis reveals microbial spore responses to sodium hypochlorite

Yang,

Yuan,

et al. 2024

Journal of Biophotonics

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“…Exposure to ultraviolet C (UV-C, 200-280 nm) light is one of the most commonly used sterilization methods (Jang et al 2022) because it is effective against bacterial cells and spores with little to no effect on filtration efficiency in respiratory devices. (Lindsley et al 2015;Nguyen et al 2021;Paul et al 2020). The most challenging aspect of the use of UV radiation is that microorganism sterilization can vary between non-porous and porous surfaces due to a shadowing effect (Banerjee et al 2021;Kayani et al 2021).…”

Section: Introductionmentioning

confidence: 99%

Effective inactivation of Bacillus atrophaeus spores and Escherichia coli on disposable face masks using ultraviolet laser irradiation

Nguyen

Jang

et al. 2022

J Anal Sci Technol

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Due to the widespread emergence of COVID-19, face masks have become a common tool for reducing transmission risk between people, increasing the need for sterilization methods against mask-contaminated microorganisms. In this study, we measured the efficacy of ultraviolet (UV) laser irradiation (266 nm) as a sterilization technique against Bacillus atrophaeus spores and Escherichia coli on three different types of face mask. The UV laser source demonstrated high penetration of inner mask layers, inactivating microorganisms in a short time while maintaining the particle filtration efficiency of the masks. This study demonstrates that UV laser irradiation is an efficient sterilization method for removing pathogens from face masks.

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“…In addition, UV can also indirectly benefit biotic decomposition due to the photodegradation of recalcitrant compounds, a process known as photofacilitation (Méndez et al, 2019). However, most research and experimental practices show that UV radiation sterilizes microorganisms by destroying their cells and spores (Johnson, 2003; Nguyen et al, 2021; Taylor et al, 2020). Thus, microbial activity is likely reduced under UV radiation (Duguay & Klironomos, 2000).…”

Section: Introductionmentioning

confidence: 99%

“…However, most research and experimental practices show that UV radiation sterilizes microorganisms by destroying their cells and spores (Johnson, 2003;Nguyen et al, 2021;Taylor et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

UV radiation doubles microbial degradation of standing litter in a subtropical forest

et al. 2022

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1. UV radiation has been recognized as a direct driver of litter decomposition by photodegrading organic matter in dryland ecosystems. However, the importance and mechanism of UV radiation on litter decomposition, especially on standing litter, in humid forest ecosystems remain unclear.2. We conducted a factorial experiment in a humid subtropical forest gap, manipulating the effects of UV radiation on the decomposition of standing litter under different microbial conditions.3. After 366 days of standing incubation, under normal conditions (UV pass with microorganisms), up to 40.63% of the litter mass was lost. However, under a UV pass without microorganisms, litter mass loss was only 16.30%. Under a UV block, the mass loss of litter with microorganisms was 27.68% and that of litter without microorganisms was 15.54%. Without microorganisms, UV radiation had no significant effect on the mass loss of litter carbon. However, UV radiation increased the DOC concentration of litter. And in the presence of microorganisms, UV radiation contributed to an increased mass loss of lignin by 16.72% and of cellulose by 14.75%. No negative effects of UV radiation on microorganisms were observed. These results suggest that UV radiation increased the net mass loss of litter by 106.67%, and this doubling promotion was achieved through microbial degradation.4. Synthesis. The increase in microbial degradation under UV radiation may be linked to the increased degradability of lignin and cellulose caused by photodegradation. Our study indicates that direct photodegradation by UV radiation could be weak in subtropical forests, but UV photofacilitation generates rapid turnover of carbon in this system.

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Sterilization effects of UV laser irradiation onBacillus atrophaeusspore viability, structure, and proteins

Abstract: A UV-C laser is capable of sterilizing Bacillus atrophaeus spores.

Cited by 8 publications

References 64 publications

Single‐cell analysis reveals microbial spore responses to sodium hypochlorite

Single‐cell analysis reveals microbial spore responses to sodium hypochlorite

Effective inactivation of Bacillus atrophaeus spores and Escherichia coli on disposable face masks using ultraviolet laser irradiation

UV radiation doubles microbial degradation of standing litter in a subtropical forest

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