Sterile UGA nonsense mutants of fission yeast

Leupold, Urs; Sipiczki, Matthias

doi:10.1007/bf00312767

Cited by 14 publications

(9 citation statements)

References 40 publications

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“…Instead, mating is most efficiently triggered by nitrogen starvation, and immediately followed by meiosis and sporulation [14], [15]. From classical genetic screens, about 20 mating genes have been identified in S. pombe , which are called ste (for sterile) or ral (for ras -like) genes [16 and references therein]. According to PomBase [17], 15 ste and ral genes have been cloned thus far.…”

Section: Resultsmentioning

confidence: 99%

Global Analysis of Fission Yeast Mating Genes Reveals New Autophagy Factors

et al. 2013

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Macroautophagy (autophagy) is crucial for cell survival during starvation and plays important roles in animal development and human diseases. Molecular understanding of autophagy has mainly come from the budding yeast Saccharomyces cerevisiae, and it remains unclear to what extent the mechanisms are the same in other organisms. Here, through screening the mating phenotype of a genome-wide deletion collection of the fission yeast Schizosaccharomyces pombe, we obtained a comprehensive catalog of autophagy genes in this highly tractable organism, including genes encoding three heretofore unidentified core Atg proteins, Atg10, Atg14, and Atg16, and two novel factors, Ctl1 and Fsc1. We systematically examined the subcellular localization of fission yeast autophagy factors for the first time and characterized the phenotypes of their mutants, thereby uncovering both similarities and differences between the two yeasts. Unlike budding yeast, all three Atg18/WIPI proteins in fission yeast are essential for autophagy, and we found that they play different roles, with Atg18a uniquely required for the targeting of the Atg12–Atg5·Atg16 complex. Our investigation of the two novel factors revealed unforeseen autophagy mechanisms. The choline transporter-like protein Ctl1 interacts with Atg9 and is required for autophagosome formation. The fasciclin domain protein Fsc1 localizes to the vacuole membrane and is required for autophagosome-vacuole fusion but not other vacuolar fusion events. Our study sheds new light on the evolutionary diversity of the autophagy machinery and establishes the fission yeast as a useful model for dissecting the mechanisms of autophagy.

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Section: Resultsmentioning

confidence: 99%

Global Analysis of Fission Yeast Mating Genes Reveals New Autophagy Factors

et al. 2013

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“…Many sporulation‐deficient mutants and sterility mutants have been isolated and characterized in Sz. pombe (Bresch et al ., 1968; Leupold and Sipiczki, 1991; Thuriauz et al ., 1980), and most of these have exhibited defects in the regulation of the distinct steps of sporulation or meiosis. However, there have been no investigations on the relationship between sexual conjugation and cell surface glycosylation in Sz.…”

Section: Resultsmentioning

confidence: 99%

Characterization of a Schizosaccharomyces pombe mutant deficient in UDP‐galactose transport activity

Tanaka

Konomi

Osumi

et al. 2001

Yeast

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In fission yeast, Schizosaccharomyces pombe, the carbohydrate components of the cell wall consist of galactomannan, unlike in Saccharomyces cerevisiae. We previously found that the disruption of gms1 + , a gene encoding the UDP-galactose transporter required for the synthesis of galactomannan, led to the complete defect of cell surface galactosylation in Sz. pombe. The Dgms1 strain is therefore useful for the analysis of physiological properties of galactose residues in Sz. pombe. The deletion strain of gms1 + was viable; however, it showed an aberrant cell morphology and increased sensitivities to digestion with b-glucanase and to various drugs, such as hygromycin B, sodium orthovanadate and Calcofluor white. A reduction of galactomannan layers of the cell wall in the Dgms1 strain was observed by scanning and transmission electron microscopic analyses. The addition of osmotic stabilizer suppressed the morphologic defect of the Dgms1 cells, while other phenotypes were weakly suppressed. The Dgms1 (h 90 ) strain was incapable of sexual conjugation during nutritional starvation. These results suggest that the cell surface galactosylation is required not only for non-sexual flocculation but also for sexual conjugation in Sz. pombe.

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“…As described below in detail, the ste9 mutant conformed to this criterion and exhibited defects indistinguishable from those of the rum1 mutant. The ste9 mutant was identified previously (Leupold and Sipiczki, 1991), but its molecular characterization has not been attempted. To determine its coding product, the ste9 ϩ gene was isolated by rescuing the sterility of its mutant.…”

Section: Resultsmentioning

confidence: 99%

“…Analysis of the DNA content of the cells at 30°C revealed that the apparent 1C peak corresponding to the G 1 population was seen in the cdc10 ts strain, but no such population was found in the ste9 cdc10 ts strain ( Figure 4B). The DNA profiles of both strains at 32°C were also quite different, in that appearance of the Leupold and Sipiczki (1991). ste9-59 is a novel allele encoding partially active protein (our unpublished result, see text).…”

Section: Ste9 and Wee1 Mutations Cause Synthetic Lethalitymentioning

confidence: 85%

Fission Yeast Ste9, a Homolog of Hct1/Cdh1 and Fizzy-related, Is a Novel Negative Regulator of Cell Cycle Progression during G₁-Phase

Kitamura

Maekawa

Shimoda

1998

MBoC

110

125

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When proliferating fission yeast cells are exposed to nitrogen starvation, they initiate conjugation and differentiate into ascospores. Cell cycle arrest in the G1-phase is one of the prerequisites for cell differentiation, because conjugation occurs only in the pre-Start G1-phase. The role ofste9 + in the cell cycle progression was investigated. Ste9 is a WD-repeat protein that is highly homologous to Hct1/Cdh1 and Fizzy-related. The ste9 mutants were sterile because they were defective in cell cycle arrest in the G1-phase upon starvation. Sterility was partially suppressed by the mutation in cig2 that encoded the major G1/S cyclin. Although cells lacking Ste9 function grow normally, the ste9 mutation was synthetically lethal with the wee1 mutation. In the double mutants ofste9 cdc10 ts, cells arrested in G1-phase at the restrictive temperature, but the level of mitotic cyclin (Cdc13) did not decrease. In these cells, abortive mitosis occurred from the pre-Start G1-phase. Overexpression of Ste9 decreased the Cdc13 protein level and the H1-histone kinase activity. In these cells, mitosis was inhibited and an extra round of DNA replication occurred. Ste9 regulates G1 progression possibly by controlling the amount of the mitotic cyclin in the G1-phase.

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Sterile UGA nonsense mutants of fission yeast

Cited by 14 publications

References 40 publications

Global Analysis of Fission Yeast Mating Genes Reveals New Autophagy Factors

Global Analysis of Fission Yeast Mating Genes Reveals New Autophagy Factors

Characterization of a Schizosaccharomyces pombe mutant deficient in UDP‐galactose transport activity

Fission Yeast Ste9, a Homolog of Hct1/Cdh1 and Fizzy-related, Is a Novel Negative Regulator of Cell Cycle Progression during G₁-Phase

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Sterile UGA nonsense mutants of fission yeast

Cited by 14 publications

References 40 publications

Global Analysis of Fission Yeast Mating Genes Reveals New Autophagy Factors

Global Analysis of Fission Yeast Mating Genes Reveals New Autophagy Factors

Characterization of a Schizosaccharomyces pombe mutant deficient in UDP‐galactose transport activity

Fission Yeast Ste9, a Homolog of Hct1/Cdh1 and Fizzy-related, Is a Novel Negative Regulator of Cell Cycle Progression during G1-Phase

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Fission Yeast Ste9, a Homolog of Hct1/Cdh1 and Fizzy-related, Is a Novel Negative Regulator of Cell Cycle Progression during G₁-Phase