Sterile Insect Technique (SIT) and Its Applications

Bourtzis, Kostas; Vreysen, Marc J. B.

doi:10.3390/insects12070638

Cited by 50 publications

(33 citation statements)

References 37 publications

(36 reference statements)

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“…SIT is an environmentally friendly pest management strategy that hinders pest propagation by releasing large numbers of sterile insects into the wild [ 61 , 62 ]. Severe mutations in the external genitalia as a result of dsx manipulation have been reported in multiple Lepidopterans [ 34 , 36 , 44 ].…”

Section: Discussionmentioning

confidence: 99%

CRISPR/Cas9-Mediated Mutagenesis of Sex-Specific Doublesex Splicing Variants Leads to Sterility in Spodoptera frugiperda, a Global Invasive Pest

Wang

et al. 2022

Cells

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Spodoptera frugiperda (J. E. Smith), an emerging invasive pest worldwide, has posed a serious agricultural threat to the newly invaded areas. Although somatic sex differentiation is fundamentally conserved among insects, the sex determination cascade in S. frugiperda is largely unknown. In this study, we cloned and functionally characterized Doublesex (dsx), a “molecular switch” modulating sexual dimorphism in S. frugiperda using male- and female-specific isoforms. Given that Lepidoptera is recalcitrant to RNAi, CRISPR/Cas9-mediated mutagenesis was employed to construct S. frugiperda mutants. Specifically, we designed target sites on exons 2, 4, and 5 to eliminate the common, female-specific, and male-specific regions of S. frugiperda dsx (Sfdsx), respectively. As expected, abnormal development of both the external and internal genitalia was observed during the pupal and adult stages. Interestingly, knocking out sex-specific dsx variants in S. frugiperda led to significantly reduced fecundity and fertility in adults of corresponding sex. Our combined results not only confirm the conserved function of dsx in S. frugiperda sex differentiation but also provide empirical evidence for dsx as a potential target for the Sterile Insect Technique (SIT) to combat this globally invasive pest in a sustainable and environmentally friendly way.

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Section: Discussionmentioning

confidence: 99%

CRISPR/Cas9-Mediated Mutagenesis of Sex-Specific Doublesex Splicing Variants Leads to Sterility in Spodoptera frugiperda, a Global Invasive Pest

Wang

et al. 2022

Cells

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“…A complete repertoire of the SIT programmes attempted and those currently running is reported in the International Database on Insect Disinfestation and Sterilization (IDIDAS) [11]. The sterile insect control system has now been extended to mosquito control through advances in mass rearing and delivery techniques that make operational implementation more practical [12,13,14,15].…”

Section: Challenges In Area-wide Sit Deployment Against Mosquitoesmentioning

confidence: 99%

Safety, regulatory and environmental issues with sterile insect technique-based mosquito vector control in European countries

BELLINI¹

2022

Rev. Sci. Tech. OIE

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The globalisation of trade is opening the way to the spread of species in new regions where they may cause negative impacts. Invasive mosquito species such as Aedes aegypti and Aedes albopictus are raising concern for their capacity to transmit several arboviruses of public health and veterinary importance. Currently available integrated vector management measures do not achieve satisfactory results when deployed against these urban mosquitoes. Moreover, insecticides are losing their effectiveness owing to the resistance developed by the target species. Policies regulating the use of insecticides are progressively restricting their market availability and this trend is expected to continue.Genetic control methods, such as the sterile insect technique (SIT), based on the use of irradiation to sterilise male mosquitoes, are showing good efficacy in pilot trials at local scales in some Aedes albopictus-colonised urban areas in Europe, without any negative effects. The main limitation is the cost, which may be significantly reduced through the introduction of automation in the mass rearing and drone technology in the field release. These technological advancements require substantial investments at a scale that can only be achieved with centralised production and extensive distribution, which in turn may be granted only if the authorisation frameworks, including the regulation of international transportation and aerial release in an urban setting, are clarified and matured.

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“…In this case, it was shaped like a longitudinal suture on the female pupae's abdominal segments and as a short slit covered by two semicircular lumps on the male pupae. Nowadays, sterile insect techniques have become common in pest management programs by mating disruption (Marec 2019;Bourtzis and Vreysen 2021), in which early sex determination of insects is very important. Accordingly, distinguishing male and female pupae through morphological characteristics will contribute to the easier application of this technique.…”

Section: Pupaementioning

confidence: 99%

Morphology, biology, and damage behaviors of nutsedge borer (Bactra venosana Zeller) in Tra Vinh Province, Vietnam

et al. 2022

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Abstract. Nguyen HU, Phan QN, Dang HT, Son TTN, Nguyen HN, Nguyen THT. 2021. Morphology, biology, and damage behaviors of nutsedge borer (Bactra venosana Zeller) in Tra Vinh Province, Vietnam. Biodiversitas 23: 733-741. Nutsedge borer, Bactra venosana Zeller (Lepidoptera: Tortricidae), is a common pest of sedge in Tra Vinh Province. To control this species, its morphology, biology, and behaviors were investigated in Tra Vinh Province. The study was conducted with the aim of determining the information related to the Bactra venosana, as a basis for developing a strategy to manage this species by means of safety solutions. The study was carried out by surveying the behavior of nutsedge borer in sedge fields and determining their morphological and biological characteristics under laboratory conditions. The results showed that the eggs were 1.06 ± 0.17 mm (in length), 0.84 ± 0.16 (in width), oval, and white after turning pale yellow. Larvae progressed through 5 instars with body length and width of 1.20 ± 0.18 mm to 11.3 ± 1.23 mm and from 0.25 ± 0.05 to 1.05 ± 0.25 mm, respectively. Newly hatched larvae were dark yellow, and pupae were pale green. The male pupa was 7.75 ± 0.68 mm long, while the female was 8.46 ± 0.93 mm long, light green, and dark brown about to mature. The male adult of B. venosana was usually gray-brown and 7.70 ± 0.60 mm in body length, whereas the female adult was silver-gray, 8.41 ± 0.86 mm. The life cycle passed 35.9 ± 2.55 days in laboratory conditions. Under field conditions, eggs were laid scattered on the surface of the host plants. Larvae bored inside the stems of sedge. Newly damaged sedge plants had no symptoms at first whose bodies got soft, turned from light green to pale yellow, and gradually wilted and died.

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Sterile Insect Technique (SIT) and Its Applications

Cited by 50 publications

References 37 publications

CRISPR/Cas9-Mediated Mutagenesis of Sex-Specific Doublesex Splicing Variants Leads to Sterility in Spodoptera frugiperda, a Global Invasive Pest

CRISPR/Cas9-Mediated Mutagenesis of Sex-Specific Doublesex Splicing Variants Leads to Sterility in Spodoptera frugiperda, a Global Invasive Pest

Safety, regulatory and environmental issues with sterile insect technique-based mosquito vector control in European countries

Morphology, biology, and damage behaviors of nutsedge borer (Bactra venosana Zeller) in Tra Vinh Province, Vietnam

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