Sterile Insect Technique

Dyck, V. A.; Hendrichs, Jorge; Robinson, A. S.

doi:10.1201/9781003035572

Cited by 173 publications

(114 citation statements)

References 11 publications

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“…If mating occurs, no offspring is produced. Repeated releases lead to a progressive decline in the wild population [9]. The continuous release of a large number of competitive sterile males is therefore crucial.…”

Section: Introductionmentioning

confidence: 99%

The Possible Role of Microorganisms in Mosquito Mass Rearing

Chersoni

Checcucci

Malfacini

et al. 2021

Insects

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In Europe, one of the most significant mosquitoes of public health importance is Aedes albopictus (Skuse), an allochthonous species of Asian origin. One of the most promising control methods against Aedes albopictus is the sterile insect technique (SIT), which consists of mass rearing the target species, separation of males from females, and male exposure to sterilizing ionizing radiation. Once released in the environment, the sterile males are expected to search for wild females to mate with. If mating occurs, no offspring is produced. The quality of sterile males is a crucial aspect in SIT programs in order to optimize effectiveness and limit production costs. The integration of probiotic microorganisms in larval and adult mosquito diets could enhance the quality parameters of the released sterile males. In this review, we attempt to give the most representative picture of the present knowledge on the relationships between gut microbiota of mosquitoes and the natural or artificial larval diet. Furthermore, the possible use of probiotic microorganisms for mosquito larvae rearing is explored. Based on the limited amount of data found in the literature, we hypothesize that a better understanding of the interaction between mosquitoes and their microbiota may bring significant improvements in mosquito mass rearing for SIT purposes.

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Section: Introductionmentioning

confidence: 99%

The Possible Role of Microorganisms in Mosquito Mass Rearing

Chersoni

Checcucci

Malfacini

et al. 2021

Insects

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“…Sterile insect technique (SIT), as a component of area-wide integrated pest management (AW-IPM) programmes, has been successfully applied to suppress or even locally eradicate insect populations of several major agricultural and livestock pest species during the last seventy years (Vreysen et al, 2007;Dyck et al, 2021). Based on these successful applications, recent efforts have focused on the development and validation of the SIT package to suppress populations of mosquito disease vectors including Ae.…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, several small-scale field trials have provided quite encouraging results suggesting that SIT has the potential to suppress Aedes mosquito populations (Bellini et al, 2013;Kittayapong et al, 2018Kittayapong et al, , 2019Zheng et al, 2019). One of the key elements for the successful application of SIT to suppress populations of insect pests and disease vectors, including mosquitoes, is the production of highquality sterile males Dyck et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…It has been shown that mass rearing, handling, transport, release and sterilization may affect the male mating competitiveness (Bellini et al, 2013;Madakacherry et al, 2014;Maïga et al, 2014;Yamada et al, 2014;Diallo et al, 2019;Du et al, 2019;Zheng et al, 2019;Dyck et al, 2021). In addition, several studies have suggested that changes in the male mating competitiveness may be associated with radiation-induced changes in the gut-associated bacteriome of SIT target species (Ami et al, 2010;Gavriel et al, 2010;Cai et al, 2018;Asimakis et al, 2019;Woruba et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

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The Effect of Radiation on the Gut Bacteriome of Aedes albopictus

et al. 2021

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The sterile insect technique (SIT) has been developed as a component of area-wide integrated pest management approaches to control the populations of Aedes albopictus, a mosquito vector capable of transmission of dengue, Zika and chikungunya viruses. One of the key factors for the success of SIT is the requirement of high biological quality sterile males, which upon their release would be able to compete with wild males for matings with wild females in the field. In insects, gut bacteriome have played a catalytic role during evolution significantly affecting several aspects of their biology and ecology. Given the importance of gut-associated bacterial species for the overall ecological fitness and biological quality of their hosts, it is of interest to understand the effects of radiation on the gut-associated bacteriome of Ae. albopictus. In this study, the effect of radiation on the composition and density levels of the gut-associated bacterial species at the pupal stage as well as at 1- and 4-day-old males and females was studied using 16S rRNA gene-based next generation sequencing (NGS) and quantitative PCR (qPCR) approaches. Age, diet, sex, and radiation were shown to affect the gut-associated bacterial communities, with age having the highest impact triggering significant changes on bacterial diversity and clustering among pupae, 1- and 4-day-old adult samples. qPCR analysis revealed that the relative density levels of Aeromonas are higher in male samples compared to all other samples and that the irradiation triggers an increase in the density levels of both Aeromonas and Elizabethkingia in the mosquito gut at specific stages. Our results suggest that Aeromonas could potentially be used as probiotics to enhance protandry and sex separation in support of SIT applications against Ae. albopictus, while the functional role of Elizabethkingia in respect to oxidative stress and damage in irradiated mosquitoes needs further investigation.

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“…The sterile insect technique (SIT) is used to suppress insect populations by releasing large numbers of individuals (chiefly males) that mate in the wild but sterilize the broods of their mates ( Knipling, 1955 ; Knipling, 1959 ; Knipling, 1960 ; Davidson, 1974 ; Pal, 1974 ; Klassen & Curtis, 2005 ; Dyck, Hendrichs & Robinson, 2005 ; Dyck, Hendrichs & Robinson, 2021 ). The SIT has many successes and failures over a range of species attempted.…”

Section: Introductionmentioning

confidence: 99%

The sterile insect technique is protected from evolution of mate discrimination

Bull

Gomulkiewicz

2022

PeerJ

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Background The sterile insect technique (SIT) has been used to suppress and even extinguish pest insect populations. The method involves releasing artificially reared insects (usually males) that, when mating with wild individuals, sterilize the broods. If administered on a large enough scale, the sterility can collapse the population. Precedents from other forms of population suppression, especially chemicals, raise the possibility of resistance evolving against the SIT. Here, we consider resistance in the form of evolution of female discrimination to avoid mating with sterile males. Is resistance evolution expected? Methods We offer mathematical models to consider the dynamics of this process. Most of our models assume a constant-release protocol, in which the same density of males is released every generation, regardless of wild male density. A few models instead assume proportional release, in which sterile releases are adjusted to be a constant proportion of wild males. Results We generally find that the evolution of female discrimination, although favored by selection, will often be too slow to halt population collapse when a constant-release implementation of the SIT is applied appropriately and continually. The accelerating efficacy of sterile males in dominating matings as the population collapses works equally against discriminating females as against non-discriminating females, and rare genes for discrimination are too slow to ascend to prevent the loss of females that discriminate. Even when migration from source populations sustains the treated population, continued application of the SIT can prevent evolution of discrimination. However, periodic premature cessation of the SIT does allow discrimination to evolve. Likewise, use of a ‘proportional-release’ protocol is also prone to escape from extinction if discriminating genotypes exist in the population, even if those genotypes are initially rare. Overall, the SIT is robust against the evolution of mate discrimination provided care is taken to avoid some basic pitfalls. The models here provide insight for designing programs to avoid those pitfalls.

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Sterile Insect Technique

Cited by 173 publications

References 11 publications

The Possible Role of Microorganisms in Mosquito Mass Rearing

The Possible Role of Microorganisms in Mosquito Mass Rearing

The Effect of Radiation on the Gut Bacteriome of Aedes albopictus

The sterile insect technique is protected from evolution of mate discrimination

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