Stereospecific binding of estrogens in the rat uterus

Noteboom, William D.; Gorski, Jack

doi:10.1016/0003-9861(65)90235-3

Cited by 280 publications

(69 citation statements)

References 19 publications

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“…However, many notable differences exist between chick oviduct and mammalian uterus . For example, rat uterus possesses a marked ability to accumulate estrogen due to the presence of a binding substance(s) as shown by Jensen and his coworkers (48,49), and by Gorski and his collaborators (50,51), whereas chick oviduct does not seem to possess such an apparent feature (52) . This apparent lack of a binding substance might be one of the reasons why the minimum threshold dose of estrogen to cause a similar extent of increase in wet weight in 3-5 days is much higher in chick oviduct (1 .6 mg/kg body weight) (2) than in rat uterus (1 µg/kg body weight) (53) .…”

Section: Discussionmentioning

confidence: 97%

Interaction of Estrogen and Progesterone in Chick Oviduct Development

Oka

Schimke

1969

The Journal of Cell Biology

212

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Daily administration of estrogen to immature female chicks results in marked oviduct growth and appearance of characteristic tubular gland cells which contain lysozyme . Although a rapid increase in total DNA and RNA content begins within 24 hr, cell specific protein, lysozyme, is first detectable after 3 days of estrogen . Progesterone administered concomitantly with estrogen antagonizes the estrogen-induced tissue growth as well as appearance of tubular gland cells and their specific products, lysozyme and ovalbumin . When the initiation of progesterone administration is delayed for progressively longer periods (days) during estrogen treatment, proportionally greater growth occurs with more lysozyme and tubular gland cells after 5 days of total treatment. Progesterone does not inhibit the estrogen-stimulated increase in uptake of a-aminoisobutyric acid and water by oviduct occurring within 24 hr or the estrogen-induced increase in total lipid, phospholipid, and phosphoprotein content of serum . The above results of progesterone antagonism can best be explained by the hypothesis that progesterone inhibits the initial proliferation of cells which become tubular gland cells but does not antagonize the subsequent cytodifferentiation leading to the synthesis of lysozyme and ovalbumin once such cell proliferation has occurred .

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Section: Discussionmentioning

confidence: 97%

Interaction of Estrogen and Progesterone in Chick Oviduct Development

Oka

Schimke

1969

The Journal of Cell Biology

212

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“…The purification of receptor proteins and the availability of respective antibodies permitted the application of immune techniques, that is Western blotting, immunofluorescence, confocal and immunogold electron microscopy, for the identification of glucocorticoid (GR), estrogen (ER), androgen (AR), and thyroid hormone (TR) receptors in mitochondria (Fig. 2, Table 1) (37,(42)(43)(44)(45)(46)(47)(48)(49)(50)(51)(52)(53)(54)(55)(56)(57)(58)(59)(60)(66)(67)(68)(69).…”

Section: The Detection Of Steroid and Thyroid Receptors In Mitochondriamentioning

confidence: 99%

“…First reports for the presence of estrogen receptors in mitochondria were based on the distribution and binding of radioactively labeled ligands (52,66,67). Later, using immunotechniques, ER was detected in mitochondria of rat uterine and ovarian cells (53), of MCF-7 breast cancer cells (16,57), of cultured human lens epithelial cells (55), and of rat hippocampus and neuronal cells (56).…”

Section: The Detection Of Steroid and Thyroid Receptors In Mitochondriamentioning

confidence: 99%

Steroid and thyroid hormone receptors in mitochondria

2008

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SummaryReceptors for glucocorticoids, estrogens, androgens, and thyroid hormones have been detected in mitochondria of various cell types by Western blotting, immunofluorescence labeling, confocal microscopy, and immunogold electron microscopy. A role of these receptors in mitochondrial transcription, OXPHOS biosynthesis, and apoptosis is now being revealed. Steroid and thyroid hormones regulate energy production, inducing nuclear and mitochondrial OXPHOS genes by way of cognate receptors. In addition to the action of the nuclearly localized receptors on nuclear OXPHOS gene transcription, a parallel direct action of the mitochondrially localized receptors on mitochondrial transcription has been demonstrated. The coordination of transcription activation in nuclei and mitochondria by the respective receptors is in part realized by their binding to common trans acting elements in the two genomes. Recent evidence points to a role of the mitochondrial receptors in cell survival and apoptosis, exerted by genomic and nongenomic mechanisms. The identification of additional receptors of the superfamily of nuclear receptors and of other nuclear transcription factors in mitochondria increases their arsenal of regulatory molecules and further underlines the central role of these organelles in the integration of growth, metabolic, and cell survival signals.2008 IUBMB IUBMB Life, 60(4): [210][211][212][213][214][215][216][217][218][219][220][221][222][223] 2008

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“…It has been known for some time that oestrogens are taken up by uterine tissue in vitro (Stone and Baggett 1965b;Jensen et al 1966), and that a subcellular distribution of steroids results which is similar to that observed after in vivo administration of oestrogens (Noteboom and Gorski 1965). More importantly, after in vitro incubation of uterine tissue with tritiated oestrogens, the radioactivity is associated with the same 'cytosol' and 'nuclear' macromolecules as after in vivo administration (Jensen et al 1967).…”

Section: Introductionmentioning

confidence: 78%

Uptake and Metabolism of Tritiated 17ß-Oestradiol and Oestrone by Rabbit Uterine Tissue in vitro

Gabb¹,

Stone²

1974

Aust. Jnl. Of Bio. Sci.

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To determine whether the established capability of rabbit uterine tissue to interconvert 17 p-oestradiol and oestrone might have some effect on the mode of action of the oestrogens in this organ, the in vitro interconversion of [3H]-17p-oestradiol and [3H]oestrone by rabbit endometrial and myometrial tissue was investigated and the identity of radiometabolites in 'soluble, 'mitochondrial-microsomal' a'nd 'nuclear' preparations was studied. Both endometrial and myometrial tissue were found to be capable of oxidoreduction of the oestrogens, the equilibrium of the reaction favouring the reduction of oestrone. Irrespective of the tissue--steroid combination studied, the greater part of the radioactivity in all fractions was associated with 17p-oestradiol. The relative proportions of oestrone varied between fractions, the nuclear preparation consistently showing a lower proportion of oestrone than the other fractions. Sephade

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Stereospecific binding of estrogens in the rat uterus

Cited by 280 publications

References 19 publications

Interaction of Estrogen and Progesterone in Chick Oviduct Development

Interaction of Estrogen and Progesterone in Chick Oviduct Development

Steroid and thyroid hormone receptors in mitochondria

Uptake and Metabolism of Tritiated 17ß-Oestradiol and Oestrone by Rabbit Uterine Tissue in vitro

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