2003
DOI: 10.1007/bf02497471
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Stereospecific analysis of flurbiprofen and its major metabolites in plasma and urine by chiral-phase liquid chromatography

Abstract: Key WordsColumn liquid chromatography Enantiospecific chromatography Enantioselective disposition Flurbiprofen enantiomers and metabolites SummaryDirect chiral-phase HPLC methods have been developed for the determination of flurbiprofen and its major metabolites, namely 4'-hydroxyflurbiprofen and 3'-hydroxy-4'-methoxyflurbiprofen, in biological fluids using a derivatized amylose chiral stationary phase (CSP; ChiralpakAD). Quantification of all three analytes, both free and conjugated, in urine was carried out … Show more

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Cited by 5 publications
(3 citation statements)
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“…Full details of the analytical methodologies employed, including their development, validation and preliminary application to samples of biological origin, have been reported by Patel et al (2002Patel et al ( , 2003. Brief details of each method are presented below.…”
Section: Methodsmentioning
confidence: 99%
“…Full details of the analytical methodologies employed, including their development, validation and preliminary application to samples of biological origin, have been reported by Patel et al (2002Patel et al ( , 2003. Brief details of each method are presented below.…”
Section: Methodsmentioning
confidence: 99%
“…Examples of flurbiprofen enantiomers separation, using a Chiralpak AD stationary phase as chiral selector, can be found in the literature. Patel et al used a hexane/isopropyl alcohol/trifluoracetic acid (90/10/0.05%, v/v) mobile phase composition and obtained a selectivity value of 1.7 [13,14]. A similar mobile phase composition (95/5/1) was used by Booth and collaborators, using the same stationary phase [15].…”
Section: Introductionmentioning
confidence: 94%
“…3). We have previously reported on various chromatographic approaches for the separation and resolution of the drug and its two major urinary metabolites [141] and developed methodology for the determination of the stereochemical composition of the drug and its major plasma and urinary metabolites following administration of the racemate to man [142,143]. In order to develop an alternative rapid approach, and exploit the reported advantages of CE, to this analytical problem we have recently examined the resolution of flurbiprofen and Reproducibility of the electrophoretic analysis of flurbiprofen and its metabolites in urine.…”
mentioning
confidence: 99%