Stereoselective effects of 3-hydroxybutyrate on glucose utilization of rat cardiomyocytes

Highly efficient ketone body treatment in multiple acyl-CoA dehydrogenase deficiency–related leukodystrophy

“…Little is known about the uptake and metabolization of free L-3-hydroxybutyrate by peripheral organs and its physiological importance in humans. In rats, it has been shown, that the L-enantiomer is produced by the liver (17) and can be utilized by the developing brain (18) and that there is a stereoselective effect of the enantiomers in cardiomyocytes (19). L-3-hydroxybutyrate may be thus converted to physiological ketone bodies, diverted to biosynthesis of lipids, sterols and other complex molecules or fully metabolized to CO2 by the Krebs cycle (20,21).…”

Depolarizing Actions of GABA in Immature Neurons Depend Neither on Ketone Bodies Nor on Pyruvate

“…In addition, the argument that 4 mM DL-BHB is equivalent to 2 mM active D-BHB (Rheims et al, 2009) is invalidated by the observations that L-BHB exerts complex biological actions (Moore et al, 1976;Webber and Edmond, 1977;Herzberg and Gad, 1984;Eaton et al, 2003;Tsai et al, 2006;Chou et al, 2008). Also, ketogenic diet reduces glucose utilization by 10% per millimolar plasma ketone bodies (Robinson and Williamson, 1980;Harding and Charlton, 1990;LaManna et al, 2009), hampering the interpretation and relevance of observations made using ACSF with glucose and DL-BHB.…”

Enantioselective determination of 3-hydroxybutyrate in the tissues of normal and streptozotocin-induced diabetic rats of different ages

“…We have previously reported that d-and l-3HB were detected in myocardial cell lines; d-3HB had an inhibitory effect, while l-3HB had the opposite effect on glucose utilization [8]. In addition to our previous in vitro study [8], the present study employed an in vivo disease model of diabetes, streptozotocin (STZ)-induced diabetic rats, to determine the concentration of d-and l-3HB in various rat tissues in normal and diabetic rats at different ages using our established HPLC method.…”

“…Since these different actions have been reported for d-3HB and l-3HB, we recently developed a column-switching high-performance liquid chromatography (HPLC) method to determine the concentrations of d-3HB and l-3HB in mammalian tissues [7,8]. To better understand the physiological role of the minor enantiomer, l-3HB, in maturation and development, we employed rats of different ages to observe age-dependent alterations, because postnatal changes in the utilization of ketone bodies or glucose have been reported [9].…”