Stereoscopic visualization and quantification of auricular cartilage regeneration in rabbits using multiphoton microscopy

Abstract

Multiphoton microscopy (MPM) was applied for imaging and quantifying the elastic cartilage regeneration tissue in a rabbit ear model without using labeling agents. Morphology of cells and collagen matrix were analysis, showing significant difference between regenerated and intact cartilage in cellular size and collagen distribution. The results demonstrate that high resolution images provide by MPM are consistent with the histological results, and show additional biological behavior which is not visible in standard histology. Advantages in instrumentation may lead to the application of MPM for intravital detection and treatment.

Multiscale Strain Transfer in Cartilage

“…Similar to articular cartilage, the chondrocytes in the outer regions are smaller and flatter than the cells in the intermediate and central zones, where the chondrocytes are larger and further apart (Keith et al 1977;Jessop et al 2016). Collagen type II in auricular cartilage is arranged together with a dense elastin fibre network surrounding the chondrocytes in a honeycomb like structure (Chen et al 2014;Bos et al 2018). The composition of the PCM of elastic cartilage has not been reported.…”

Label-free, three-dimensional multiphoton microscopy of the connective tissue in the anterior vaginal wall

“…Further advantagesespecially compared to confocal microscopy-are reduced scattering and absorption due to the longer excitation wavelengths and significantly reduced phototoxicity and photobleaching in out-of-focus regions [8]. In addition, MPM allows not only for qualitative but also for quantitative analysis of collagen and elastin fibers [10,11], as recently shown for, e.g., the dermis, arteries, cartilage, or heart [12][13][14].…”