Stereology of the human renal glomerulus

Elias, Hans; Hennig, A.

doi:10.1007/978-3-642-50130-2_9

Cited by 52 publications

(47 citation statements)

References 6 publications

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“…Assessment of the total number of glomeruli and of individual glomerular volume was based on the method of Elias and Hennig (4). Diameters of both the glomerular tuft and Bowman's capsule were measured separately with a transparent scale superimposed on the projected image.…”

Section: Methodsmentioning

confidence: 99%

Intracortical distribution of number and volume of glomeruli during postnatal maturation in the dog

Horster¹,

Kemler²,

Valtin³

1971

J. Clin. Invest.

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A B S T R A C T Morphometric analysis was carried out on kidneys of neonatal dogs in which function of the entire kidney and of single nephrons had been evaluated. Measurements were begun after neogenesis of nephrons had been completed, i.e., at the end of the 3rd postnatal wk. They were continued to 74 days by which time glomerular function, expressed per unit of renal weight, had reached the mature level. For statistical analysis, the cortical histogram at each age was divided into eight zones of equal depth between the capsule and corticomedullary junction.The mean total number of glomeruli in this beagle strain was 589 X 10' per kidney. The fraction of the total number of glomeruli was lowest in the subcapsular layer (3.9%) and highest (24.5%) in the zone immediately beneath from where it decreased almost linearly to a value of 4.5% in the juxtamedullary region. This numerical distribution did not change with age, which suggests that growth of nonglomerular structures proceeded at the same rate in all cortical layers.Volume of the glomerular tuft rose slightly between the subcapsular and next layer and remained constant down to the juxtamedullary region where it increased sharply. The juxtamedullary glomerulus was about 45% larger in volume than the other glomeruli. This intracortical distribution of glomerular volume did not vary between 23 and 74 days, although the volume of an individual glomerulus at each level increased slightly with age.

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Section: Methodsmentioning

confidence: 99%

Intracortical distribution of number and volume of glomeruli during postnatal maturation in the dog

Horster¹,

Kemler²,

Valtin³

1971

J. Clin. Invest.

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“…Thin (80 90 nm) sections were obtained with a Sorvall Porter-Blum MT2-B ultramicrotome, stained with lead citrate and viewed with a Philips EM-300 transmission electron microscope. Quantitation of the surface area of glomerular capillary wall available for filtration was determined using the stereological technique described by Elias and Hcitnig [9] w ith the aid of an analog to digital graphic data analyzing system (Ladd Research In dustries).…”

Section: Methodsmentioning

confidence: 99%

Quantitative Structural Aspects of the Renal Glomeruli of Hypertensive Mice

Rosenberg

Palmieri

Schlager

et al. 1982

Nephron

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Kidneys of genetically hypertensive and normotensive mice were studied with respect to kidney weight, number and volume of glomeruli, and filtration surface area. The hypertensive animals have a larger kidney weight to body weight ratio and possess fewer glomeruli per kidney. The superficial cortical glomeruli of the hypertensives are slightly smaller in volume than those of the normotensives although the filtration surface area is similar. The juxtamedullary glomeruli of the hypertensives are both smaller in volume and have less filtration surface area. The possible implications of these findings in the development and/or maintenance of hypertension are discussed.

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“…Stereological techniques are beginning to be applied to the kidney both in healthy and diseased humans [15,16,291 and have been used with some success to differentiate acute post-streptococcal glomerulonephritis and non-streptococcal glomerulonephritis, which could not be differentiated by standard techniques [ 151.…”

Section: Other Organsmentioning

confidence: 99%

Morphometric Methods in Veterinary Pathology: A Review

Reid

1980

Vet Pathol

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Abstract. The stereological procedures available for estimating volume, surface area and number of the structural components of cells and tissue are reviewed. The applications of stereology to the study of liver, mammary gland, lung and placenta are discussed with particular emphasis on the aspects of interest to veterinary pathologists. Stereology provides the cell biologist and the pathologist with a powerful tool for describing biological structure in quantitative terms at various levels of organization from the organ to the organelle.Histology occupies a central position in pathology, but has been one of the last branches to use quantitative methods routinely. In the past decade, quantitative morphology at the microscopic level has become necessary in the study of tissues and cells. Quantitation is especially valuable in studying structural and functional changes produced by disease.The use of quantitative data in the description of structural features is called morphometry; morphometric data can be obtained by various means including stereological analysis of tissue sections. Stereology is a technique based on geometric principles that allows the derivation of three-dimensional structures from two-dimensional sections of these structures. By stereological methods, the volume (V), surface (S) and number (N) of structural features in tissues and cells can be determined quantitatively.The study of biological structure, even in quantitative terms, is a sterile and obsolete science unless related to function. Cell biology has taught us that precisely defined structure is essential for life at the level of molecules, of cells and of organs, and that deviation from this may lead to disease. Cell biology also has shown that most cells contain the full spectrum of organelles, and has identified the functions of many of these. Stereological analysis of tissue sections, by determining the quantitative balance of organelles, can thus provide valuable information about structurefunction correlations and the functional capability of cells. A number of excellent reviews have dealt with the application of stereology to cell biology and human pathology [41, 48, 52, 571. This review deals with the application of stereology to veterinary pathology. 522

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Stereology of the human renal glomerulus

Cited by 52 publications

References 6 publications

Intracortical distribution of number and volume of glomeruli during postnatal maturation in the dog

Intracortical distribution of number and volume of glomeruli during postnatal maturation in the dog

Quantitative Structural Aspects of the Renal Glomeruli of Hypertensive Mice

Morphometric Methods in Veterinary Pathology: A Review

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