Stereology and histogram analysis of backscattered electron images: Age changes in bone

Boyde, A.; Elliott, JC; Jones, Siana

doi:10.1016/8756-3282(93)90142-w

Cited by 59 publications

(31 citation statements)

References 8 publications

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“…Polishing procedures give rise to microscopic surface relief due to the changing orientation of collagen in successive lamellae (Boyde, 1984a). This relief generates contrast with BSE imaging which easily overwhelms the small mean atomic contrast (Boyde and Jones, 1983;Boyde et al, 1990bBoyde et al, , 1993Howell and Boyde, 1994).…”

Section: The Specimenmentioning

confidence: 97%

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Scanning electron microscopy of bone: Instrument, specimen, and issues

Boyde

Jones

1996

Microsc. Res. Tech.

102

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Section: The Specimenmentioning

confidence: 97%

“…Thus, variations in the signal (brightness) carry information about variations in the composition of the sample (Boyde and Jones, 1983b;Boyde et al, , 1993 4,5). However, it is difficult to avoid the creation of surface relief in polishing bone samples Howell and Boyde, 1994).…”

Section: The Instrument Secondary Electron Imagingmentioning

confidence: 99%

Scanning electron microscopy of bone: Instrument, specimen, and issues

Boyde

Jones

1996

Microsc. Res. Tech.

102

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“…Complementary work by Riggs (Riggs 1990), Boyde and others (Boyde, Reid and Howell 1983;Reid and Boyde 1987;Boyde, Howell, Bromage, Elliott, Riggs, Bell, Kneissel, Reid, Jayasinghe and Jones 1992;Boyde, Elliott and Jones 1993;Boyde, Haroon, Jones and Riggs 1999;Goldman, Blayvas, Boyde, Howell, Clement and Bromage 2000;Boyde, Lovicar and Zamecnik 2004)led to improvements in the resolution and accuracy of mineral density determination in bone specimens, and allowed for correlated studies of intra-vital labels and mineralisation density. …”

Section: Image Analysismentioning

confidence: 99%

Calcified tissue structure in the distal condyle of the third metacarpal bone in young Thoroughbred horses

Doube¹

2017

Preprint

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Aims: To determine improvements in third metacarpal (Mc3) condylar microanatomy attributable to preconditioning exercise. To investigate developmental causes of Mc3 condylar fracture.Methods: Twelve Thoroughbred horses were raised at pasture; six received preconditioning exercise from 10 days. Calcein labels were administered 19 and 11 days prior to euthanasia at 18 months. Six horses also received 2 seasons of race-training and were euthanised at 3 years. Slices were taken from the distal Mc3 condyle in the frontal and dorsal-and palmar-oblique frontal planes, scanned with DXA and macerated (frontal slices) or embedded in PMMA (oblique slices).Articular calcified cartilage (ACC) and subchondral bone (SCB) in oblique slices were imaged using confocal scanning light microscopy and quantitative backscattered electron scanning electron microscopy. ACC and SCB in the palmar slice lateral parasagittal grooves were imaged using µCT and nanoindentation tested. Results: Characteristic spatial variations in ACC and SCB histomorphometric parameters were present, none of which was significantly related to preconditioning exercise. Thickened, aberrantly mineralised ACC was found in 13/24 parasagittal grooves in the palmar slices and on the sagittal ridge of 4/12 dorsal slices of 18-month-old horses. Deep to thickened ACC, SCB had an open marrow structure, having not adopted the buttress morphology of the normal SCB plate. SCB in 3-year-old horses had incorporated early ACC defects as notches in parasagittal grooves and a hyaline cartilage island in a sagittal ridge. ACC was less stiff and SCB more stiff in affected than unaffected parasagittal grooves. Chondroclastic resorption in the parasagittal groove may be retarded as early as 3-6 months, possibly due to localised inhibition of Dr Naomi Boxall has guided me through the PhD experience; as one of the several PhD students 5 Attribution I knew while an undergraduate at Massey University she gave me insight into coping with the varied challenges that postgraduate study entails. Naomi also helped correct my sometimes dubious spelling, grammar and punctuation while preparing documents for submission. Jen Swindlehurst provided a number of images of equine metacarpals from her own studies that I was kindly allowed to use in my presentations. Jen's work also (conveniently) supported my own, and it was nice to have a colleague who was so intimately familiar with the problem of condylar fracture. Several of Professor

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“…Best fit of the material constants for visco-elasto/plastic material model with damage. Value indicated by 1) was expertly determined and value indicated by 2) was not varied.…”

Section: Inverse Calculation Of Materials Parameters From Fe Model Of mentioning

confidence: 99%

“…Bone remodeling is a lifelong process composed of resorption (old bone tissue removal) and formation (aposition of new bone). For the trabecular bone, most of the remodeling process takes place on the surface of the trabecular structure, the interstitial bone (bone tissue in the middle part of trabeculae) is often excluded from remodeling [1,2]. This phenomenon has a consequence in highly nonuniform distribution of mineral content [3].…”

Section: Introductionmentioning

confidence: 99%

Nanoindentation of Human Trabecular Bone – Tissue Mechanical Properties Compared to Standard Engineering Test Methods

Jiroušek¹

2012

Nanoindentation in Materials Science

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Stereology and histogram analysis of backscattered electron images: Age changes in bone

Cited by 59 publications

References 8 publications

Scanning electron microscopy of bone: Instrument, specimen, and issues

Scanning electron microscopy of bone: Instrument, specimen, and issues

Calcified tissue structure in the distal condyle of the third metacarpal bone in young Thoroughbred horses

Nanoindentation of Human Trabecular Bone – Tissue Mechanical Properties Compared to Standard Engineering Test Methods

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