Stereological study of acinar growth in the rat parotid gland induced by isoproterenol

Onofre, Mirian Aparecida; Souza, Lélia Batista de; Campos, Ariadna; Taga, Rumio

doi:10.1016/s0003-9969(97)00025-3

Cited by 12 publications

(13 citation statements)

References 23 publications

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“…The b-adrenergic receptor agonist isoproterenol induces expression of proto-oncogenes and activates extracellular signalregulated kinases through both Gs-and Gi-dependent pathways, and induces cardiomyocyte hypertrophy by elevation of intracellular Ca 2+ level with activation of calcineurin [47]. Furthermore, Onofre et al [27] suggested that acinar cell proliferation is offset by cell deletion and therefore that the total acinar cell number may not change. In the present study, we confirmed that SMG wet weight increased approximately 2.5 times and acinar cell area was approximately 3.6 times larger after the 10th day of IPR administration compared with controls.…”

Section: Discussionmentioning

confidence: 99%

“…The detailed mechanism of gland enlargement following long-term administration of IPR is still unclear, although acinar cell proliferation [27] and hypertrophy [3,8,11,12] have both been proposed as major contributing factors. Further, it is unknown whether enlarged glands return to normal size and weight once administration of IPR has stopped.…”

Section: Introductionmentioning

confidence: 99%

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Acinar Cell Hypertrophy, Proliferation and Cell Death in Rat Submandibular Glands Induced by Long-Term Isoproterenol Administration.

Ochiai

Hashimoto

Shimono

2002

Acta Histochem. Cytochem

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The purpose of this study was to elucidate the relationship between cell proliferation and cell death of submandibular gland (SMG) acinar cells in rats treated with the b-adrenergic secretagogue isoproterenol (IPR) which elicits massive growth of salivary glands. We analyzed this process using computerized imaging analysis, 5-bromo-2'-deoxyuridine (BrdU) immunohistochemical staining, the terminal deoxyribonucleotidyl transferase (TdT)-mediated-deoxyuridine triphosphate (dUTP)-biotin nick end labeling (TUNEL) method and ultrastructural observations for 10 days of IPR treatment and for 3 weeks thereafter. Proliferation of acinar cells occurred at days 2 and 3 of IPR administration, and acinar cell hypertrophy continued during the entire 10-day treatment period. Numerous TUNEL-positive cells and vesicular structures were found in the acinar area by day 3 post-IPR administration. Ultrastructurally, vacuolated structures that resembled "degenerating apoptotic bodies" were located in and between the acinar cells. A different type of cell death, which was associated with an electronlucent and edematous cytoplasm but not with mitochondrial lysis or cell membrane destruction, was also recognized in the acinus. It is suggested that this other type of cell death may correspond to "secondary necrosis".

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Acinar Cell Hypertrophy, Proliferation and Cell Death in Rat Submandibular Glands Induced by Long-Term Isoproterenol Administration.

Ochiai

Hashimoto

Shimono

2002

Acta Histochem. Cytochem

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“…According to described methodology of Onofre et al (1997), saliva samples were collected 30 hours after the end of treatment. The animals had received two drops of 4% pilocarpine hydrochloride eye drops (Allergan pilocarpina® 4%, Allergan Produtos Farmacêuticos Ltda., Guarulhos, Brazil), to stimulate salivation.…”

Section: Sialometrymentioning

confidence: 99%

Effects of Fluoxetine and Venlafaxine on the Salivary Gland - Experimental Study

Silva¹,

Azevedo²,

Lima³

et al. 2012

Effects of Antidepressants

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“…According to described methodology for Onofre et al [21], saliva samples were collected 30 hours after the end of treatment. The animals had received two drops of 4% pilocarpine hydrochloride eye drops (Allergan pilocarpina® 4%, Allergan Produtos Farmacêuticos Ltda., Guarulhos, Brazil), to stimulate the salivation.…”

Section: Sialometrymentioning

confidence: 99%

Effects of Fluoxetine and Venlafaxine and Pilocarpine on Rat Parotid Glands

Silva

Azevedo²,

Lima³

et al. 2009

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This study assessed the effect of the antidepressants, Fluoxetine and Venlafaxine, on the size (GS), mass (M), cellular volume (CV), of rat parotid salivary glands and salivary flow rate (SFR), as well as the secretagogue action of pilocarpine on this flow. Ninety animals were divided into 9 treatment groups with the antidepressants, antidepressants associated with the application of pilocarpine, antidepressants and physiologic serum, physiologic serum (control) and pilocarpine (positive control). Thirty hours after the end of treatment, saliva collection began, to determine the SFR. Next, the salivary glands were removed, GS and M measured, and the specimens processes for histomorphometric analysis and CV determination. The variable GS presented statistically significant increase among the groups that were treated for 30 days with Fluoxetine (p=0.0002) and Venlafaxine (p=0.0112) when compared with the group treated with physiologic serum (control). The group treated with Fluoxetine for 30 days revealed increase in M (p=0.0190) and diminished SFR (p=0.0031), statistically significant, when compared with the control group. CV revealed increase in acinic cells between the Fluoxetine (30 days) (p=0.0005) and Venlafaxine (30 days) (p=0.0004) groups as well, when compared with the control group. The group treated with Venlafaxine for 60 days in association with pilocarpine presented SFR similar to the control group treated for 60 days. Both Fluoxetine and Venlafaxine reduced the SFR and caused increase in CV, resulting in hypertrophy of the glands, with Fluoxetine having a more pronounced anticholinergic action. The pilocarpine increased the SFR in the group that received Venlafaxine.

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Stereological study of acinar growth in the rat parotid gland induced by isoproterenol

Cited by 12 publications

References 23 publications

Acinar Cell Hypertrophy, Proliferation and Cell Death in Rat Submandibular Glands Induced by Long-Term Isoproterenol Administration.

Acinar Cell Hypertrophy, Proliferation and Cell Death in Rat Submandibular Glands Induced by Long-Term Isoproterenol Administration.

Effects of Fluoxetine and Venlafaxine on the Salivary Gland - Experimental Study

Effects of Fluoxetine and Venlafaxine and Pilocarpine on Rat Parotid Glands

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