Stereological estimation of particle shape from vertical sections

Larsen, Nick Yin; Ziegel, Johanna F.; Nyengaard, Jens Randel; Jensen, Eva B. Vedel

doi:10.1111/jmi.12826

Cited by 2 publications

(7 citation statements)

References 20 publications

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“…The planar rotator probe has previously proven to be able to calculate volume tensors and was utilized in this study [ 55 ]. The sample area was selected perpendicular to the specified VA from the central gyral region of BA46 for each subject.…”

Section: Methodsmentioning

confidence: 99%

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Layer III pyramidal cells in the prefrontal cortex reveal morphological changes in subjects with depression, schizophrenia, and suicide

et al. 2022

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Brodmann Area 46 (BA46) has long been regarded as a hotspot of disease pathology in individuals with schizophrenia (SCH) and major depressive disorder (MDD). Pyramidal neurons in layer III of the Brodmann Area 46 (BA46) project to other cortical regions and play a fundamental role in corticocortical and thalamocortical circuits. The AutoCUTS-LM pipeline was used to study the 3-dimensional structural morphology and spatial organization of pyramidal cells. Using quantitative light microscopy, we used stereology to calculate the entire volume of layer III in BA46 and the total number and density of pyramidal cells. Volume tensors estimated by the planar rotator quantified the volume, shape, and nucleus displacement of pyramidal cells. All of these assessments were carried out in four groups of subjects: controls (C, n = 10), SCH (n = 10), MDD (n = 8), and suicide subjects with a history of depression (SU, n = 11). SCH subjects had a significantly lower somal volume, total number, and density of pyramidal neurons when compared to C and tended to show a volume reduction in layer III of BA46. When comparing MDD subjects with C, the measured parameters were inclined to follow SCH, although there was only a significant reduction in pyramidal total cell number. While no morphometric differences were observed between SU and MDD, SU had a significantly higher total number of pyramidal cells and nucleus displacement than SCH. Finally, no differences in the spatial organization of pyramidal cells were found among groups. These results suggest that despite significant morphological alterations in layer III of BA46, which may impair prefrontal connections in people with SCH and MDD, the spatial organization of pyramidal cells remains the same across the four groups and suggests no defects in neuronal migration. The increased understanding of pyramidal cell biology may provide the cellular basis for symptoms and neuroimaging observations in SCH and MDD patients.

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Section: Methodsmentioning

confidence: 99%

“…It is then possible to obtain information about the volume, shape, and orientation of X by combining volume tensors of rank 0, 1, and 2 [ 55 ].…”

Section: Methodsmentioning

confidence: 99%

Layer III pyramidal cells in the prefrontal cortex reveal morphological changes in subjects with depression, schizophrenia, and suicide

et al. 2022

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“…Under restricted isotropy, the Miles ellipsoid e (Ψ) is an ellipsoid of revolution around the vertical axis (Larsen et al . 2019, Appendix A), containing information about mean particle shape. If we let the lengths of the semi‐axes of this Miles ellipsoid e (Ψ), parallel and perpendicular to the vertical axis, be denoted a and b , respectively, then the ratio I = a / b indicates the degree of elongation of the particles in the direction of the vertical axis.…”

Section: Mean Particle Volume Tensors From Vertical Sectionsmentioning

confidence: 99%

“…If automatic segmentation of Z ∩ L is not available, the integral may be discretised, using, for example, a line grid, perpendicular to the z ‐axis, see Larsen et al . (2019, p. 11). In this paper, the method has been implemented in human brain tissue, and the precision of the estimators is assessed, using a bootstrap procedure.…”

Section: Mean Particle Volume Tensors From Vertical Sectionsmentioning

confidence: 99%

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Stereological inference on mean particle shape from vertical sections

Jensen

2021

Aus NZ J of Statistics

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It was a major breakthrough when design-based stereological methods for vertical sections were developed by Adrian Baddeley and coworkers in the 1980s. Most importantly, it was shown how to estimate in a design-based fashion surface area from observations in random vertical sections with uniform position and uniform rotation around the vertical axis. The great practical importance of these developments is due to the fact that some biostructures can only be recognised on vertical sections. Later, local design-based estimation of mean particle volume from vertical sections was developed. In the present paper, we review these important advances in stereology. Quite recently, vertical sections have gained renewed interest, since it has been shown that mean particle shape can be estimated from such sections. These new developments are also reviewed in the present paper.

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Stereological estimation of particle shape from vertical sections

Cited by 2 publications

References 20 publications

Layer III pyramidal cells in the prefrontal cortex reveal morphological changes in subjects with depression, schizophrenia, and suicide

Layer III pyramidal cells in the prefrontal cortex reveal morphological changes in subjects with depression, schizophrenia, and suicide

Stereological inference on mean particle shape from vertical sections

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