Stereolitografi ile üç boyutlu (3B) olarak biyobaskılanmış yapay kalp kapakçıklarının biyouyumluluk özelliklerinin analizi

Abstract

First time fabrication of heart valves by stereolithography  Biocompatability characterization of 3D bioprinted heart valves  Satisfactory results obtained when compared with the other techniques in literature Figure A. Flow chart of Stereolithography bioprinting of heart valves. Heart valve design a) up view b) side view, stereolithography bioprinter c) innovated printer head d) innovated reservoir, bioprinted heart valves e) side view f) up view, cell viability tests g) x40 h) x100, DAPI/ACTIN tests ı) First day, j) Third day, k) Seventh day Purpose: The purpose of this study is; fabricating 3D artificial heart valves by stereolithography that has potential to be obtained for heart valve replacements. Theory and Methods: Stereolithography has proven potential for fabricating 3D models with high structural integrity and strong mechanical properties, yet it has not been evaluated as a bioprinter. In this study stereolithography is evaluated as a bioprinter to provide hearth valves with high biomechanical characteristics. Results: As a result of the study, heart valves with high structural integrity and strong mechanical properties were fabricated. In addition more than %80 cell viability and high level of cell proliferation at the seventh day of the experiments were observed. Conclusion: As a conclusion the study proved the potential of stereolithography as a bioprinting technology. In addition it proved the capability of PEGDA: GelMA mixture for heart valve biofabrication.