Stereochemistry of Intercalation: Interaction of Daunomycin with DNA

Pigram, W. J.; Fuller, W.; Hamilton, L. D.

doi:10.1038/newbio235017a0

Cited by 276 publications

(113 citation statements)

References 13 publications

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“…intercalating agent that inhibits DNA synthesis (23,24), and its effect on cell membranes as the first target of action was also reported (25,26). Because the cycle of the pituitary cells is rather long, the damaging effect of DOX and its derivatives may be detectable by an impairment of cell functions other than mitotic division.…”

Section: Discussionmentioning

confidence: 99%

Recovery of pituitary function after treatment with a targeted cytotoxic analog of luteinizing hormone-releasing hormone

Kovács

Schally

Nagy

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

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Section: Discussionmentioning

confidence: 99%

Recovery of pituitary function after treatment with a targeted cytotoxic analog of luteinizing hormone-releasing hormone

Kovács

Schally

Nagy

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

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“…It is believed that the inhibitory effect of this compound on both normal and tumour cell growth is due to its interference with RNA synthesis by binding directly to the double-stranded DNA template [2]. X-Ray fiber diffraction studies are believed to support a binding model in which intercalation between DNA base pairs, with consequent helix unwinding, plays an important role [3]. The crystallographic analysis of doxorubicin has not been determined.…”

Section: Introductionmentioning

confidence: 99%

An extended conformational analysis of doxorubicin

Nakata

Hopfinger

1980

FEBS Letters

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“…Consistent with this, we found that CPT treatment in our system also led to an acute increase in intracellular temperature ( Figure 1E, 1G and Supplementary information, Figure S4). Interestingly, in a separate experiment, we found that another chemotherapeutic drug, doxorubicin (at 50 µM final concentration), which is a topoisomerase II inhibitor [9], did not cause any obvious intracellular temperature change ( Figure 1E and 1G). Thus, the distinct effect of these two drugs on cellular temperature likely reflects fundamental differences in their action mechanisms, the details of which remain to be further investigated.…”

mentioning

confidence: 98%

Determining intracellular temperature at single-cell level by a novel thermocouple method

et al. 2011

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Living cells can change their intramembranous temperature during cell activities such as division, gene expression, enzyme reaction, and metabolism [1,2]. Moreover, under external stimuli, such as drugs or other signals, cells may quickly change their metabolic activities, leading to acute variation of intracellular temperatures from the normal state [3,4]. However, such temperature change inside cells is usually at a small scale and is of transient nature due to the thermo-influence by the extracellular environment, rendering it rather difficult to measure using the conventional temperature detection methods. Thus, a more precise and faster-response thermometer is needed to measure single-cell temperature changes in real time, which may constitute a new layer of cellular information for studies of cellular signaling, and even clinical diagnosis and therapy.Fluorescent nanogel has been previously applied to detect changes in intracellular temperature [4]. Cells were first allowed to take up a fluorescence material and the average intracellular temperature change under a certain treatment was then determined through measuring the distinct fluorescent light intensity before and after the treatment. Such a fluorescent nanogel-based method has a number of disadvantages, including potential toxicity to cells, limit of measurement resolution (generally in the range of 0.29 °C-0.50 °C), and limit of time-scale resolution (at the scale of minutes).Thermocouple (TC) is widely used in settings that require detection of temperature changes. The TC-based detection method has a number of advantages, including the capacity for achieving high precision and rapid response. To adapt the TC method for temperature measurement at the single-cell level, one would need to develop a micro-sized TC probe (at sub-micrometer scale). The thin film method is a common approach to producing two-dimensional micro-or even nano-TCs for use in electronics industry [5]. However, such two-dimensional TCs that rely on the support of silicon chips cannot be readily used for measuring intracellular temperature. In this report, we designed a novel TC device for detecting intracellular temperature ( Figure 1A and 1B). Briefly, our TC probe is made of a sandwich structure consisting of the tungsten (W) substrate, an insulating layer made of polyurethane (PU; except at the tip), and a platinum (Pt) film (Supplementary information, Figure S1). We produced two types of TC probes, with different thickness of the Pt film (50 nm and 100 nm). In a calibration experiment with these two types of probes, we found that the 100 nm probe produced a temperature-thermoelectricity curve that showed an almost perfect match with the standard curve produced by a regular macro-sized TC, while the readings from the 50 nm probe showed deviations from the standard curve ( Figure 1B and 1C and Supplementary information, Figure S2). This result is consistent with earlier reports that when the thickness of the Pt film decreases beyond the 100 nm range, it will affect the resulti...

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Stereochemistry of Intercalation: Interaction of Daunomycin with DNA

Cited by 276 publications

References 13 publications

Recovery of pituitary function after treatment with a targeted cytotoxic analog of luteinizing hormone-releasing hormone

Recovery of pituitary function after treatment with a targeted cytotoxic analog of luteinizing hormone-releasing hormone

An extended conformational analysis of doxorubicin

Determining intracellular temperature at single-cell level by a novel thermocouple method

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