Stepwise response of MeJA-induced genes and pathways in leaves of C. roseus

Bahieldin, Ahmed; Atef, Ahmed; Edris, Sherif; Gadalla, Nour O.; Al-Matary, Mohammed; Al-Kordy, Magdy A.; Ramadan, Ahmed M.; Bafeel, Sameera O.; Alharbi, Mona G.; Al-Quwaie, Diana A.; Sabir, Jamal S. M.; Al-Zahrani, Hassan S.; Nasr, Mahmoud E; El-Domyati, Fotouh M.

doi:10.1016/j.crvi.2018.10.001

Cited by 5 publications

(5 citation statements)

References 38 publications

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“…In this study, using single cell multi-omics datasets, we discovered the first reported idioblast specific TF (CrIDM1) that regulates late-stage vinblastine biosynthetic genes (D4H and DAT). Although several TFs that regulate MIA biosynthesis have been characterized [11][12][13][14][15][16][17][18][19][20]28,29 , how the exquisite cell-type specific regulation is achieved for this pathway remains unclear. We generated the first single cell multiome dataset for C. roseus leaves to investigate gene regulation of the MIA pathway at single cell resolution.…”

Section: Discussionmentioning

confidence: 99%

“…Several transcription factors (TFs) have been identified as regulators of the MIA biosynthetic pathway in C. roseus [11][12][13][14][15][16][17][17][18][19][20] , primarily in the context of jasmonate (JA)-induction of this pathway. Major known regulators of the MIA pathway include MYC2 14,20 , bHLH iridoid synthesis (BIS) family TFs 15,18,19 , and Octadecanoid-derivative Responsive Catharanthus AP2domain (ORCA) family TFs 12,13,17,21 , all of which mediate JA induction of the MIA pathway.…”

Section: Introductionmentioning

confidence: 99%

“…2D). We first queried the expression patterns of previously studied TFs (Supplementary Table 7) [11][12][13][14][15][16][17][18][19][20]28,29 in our single cell dataset and found that only ORCA4 and BIS1/2/3 displayed cell type specific expression patterns relevant to iridoid and alkaloid biosynthetic genes (Supplementary Fig. 7A).…”

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confidence: 99%

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Cell-type aware regulatory landscapes governing monoterpene indole alkaloid biosynthesis in the medicinal plantCatharanthus roseus

Li,

Colinas,

Wood

et al. 2024

Preprint

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In plants, the biosynthetic pathways of some specialized metabolites are partitioned into specialized or rare cell types, as exemplified by the monoterpenoid indole alkaloid (MIA) pathway of Catharanthus roseus (Madagascar Periwinkle), the source of the anti-cancer compounds vinblastine and vincristine. In the leaf, the C. roseus MIA biosynthetic pathway is partitioned into three cell types with the final known steps of the pathway expressed in the rare cell type termed idioblast. How cell-type specificity of MIA biosynthesis is achieved is poorly understood. Here, we generated single-cell multi-omics data from C. roseus leaves. Integrating gene expression and chromatin accessibility profiles across single cells, as well as transcription factor (TF) binding site profiles, we constructed a cell-type-aware gene regulatory network for MIA biosynthesis. We showcased cell-type-specific transcription factors as well as cell-type-specific cis-regulatory elements. Using motif enrichment analysis, co-expression across cell types, and functional validation approaches, we discovered a novel idioblast specific TF (Idioblast MYB1, CrIDM1) that activates expression of late stage vinca alkaloid biosynthetic genes in the idioblast. These analyses not only led to the discovery of the first documented cell-type-specific TF that regulates the expression of two idioblast specific biosynthetic genes within an idioblast metabolic regulon, but also provides insights into cell-type-specific metabolic regulation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cell-type aware regulatory landscapes governing monoterpene indole alkaloid biosynthesis in the medicinal plantCatharanthus roseus

Li,

Colinas,

Wood

et al. 2024

Preprint

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“…Giri and Zaheer [46] indicated in their review that in different in vitro plant cell culture systems the addition of JA, MeJa and SA ranged in concentration from 5.0 to 500 μM; in a realistic appraisal of literature on the concentrations of elicitors revealed that, 100 μM MeJa and SA also used in majority of studies. Thiruvengadam and coauthors [47], Sun and coauthors [48], Wang and coauthors [49], Bahieldin and coauthors [50], and Gabotti and coauthors [51] have reported that elicitation in different cell suspension cultures with MeJa and SA increases the expression level of different enzymes involved in flavonoid biosynthetic pathways [52]. MeJa inhibits catalase activity and stimulates PAL increasing the flavonoids production, where SA induces gene regulation related to the biosynthesis of secondary metabolites in plants [20].…”

Section: Discussionmentioning

confidence: 99%

Optimization of Flavonoid Production in Plant Cell Culture of Thevetia peruviana Elicited with Methyl Jasmonate and Salicylic Acid

Durán

Arias-Zabala

Londoño

2021

Braz. arch. biol. technol.

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“…Several studies have shown that exogenous hormones such as salicylic acid (SA), methyl jasmonate (MeJA), ethylene (ETH), and abscisic acid (ABA), induced or otherwise, alter the biosynthesis of secondary metabolites [35][36][37][38]. Catharanthine in Catharanthus roseus [39], isoquinoline alkaloids in Dendrobium officinale PLBs [40], camptothecin (CPT) in Camptotheca acuminata [41], have been found in response to hormones on biosynthetic pathways. Nevertheless, there is still a gap in research on the effects of exogenous plant hormones on the MIA biosynthesis of G. elegans.…”

Section: Introductionmentioning

confidence: 99%

Reference Genes Screening and Gene Expression Patterns Analysis Involved in Gelsenicine Biosynthesis under Different Hormone Treatments in Gelsemium elegans

Zhang,

Mu,

Wang

et al. 2023

IJMS

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Reverse transcription quantitative polymerase chain reaction (RT-qPCR) is an accurate method for quantifying gene expression levels. Choosing appropriate reference genes to normalize the data is essential for reducing errors. Gelsemium elegans is a highly poisonous but important medicinal plant used for analgesic and anti-swelling purposes. Gelsenicine is one of the vital active ingredients, and its biosynthesis pathway remains to be determined. In this study, G. elegans leaf tissue with and without the application of one of four hormones (SA, MeJA, ETH, and ABA) known to affect gelsenicine synthesis, was analyzed using ten candidate reference genes. The gene stability was evaluated using GeNorm, NormFinder, BestKeeper, ∆CT, and RefFinder. The results showed that the optimal stable reference genes varied among the different treatments and that at least two reference genes were required for accurate quantification. The expression patterns of 15 genes related to the gelsenicine upstream biosynthesis pathway was determined by RT-qPCR using the relevant reference genes identified. Three genes 8-HGO, LAMT, and STR, were found to have a strong correlation with the amount of gelsenicine measured in the different samples. This research is the first study to examine the reference genes of G. elegans under different hormone treatments and will be useful for future molecular analyses of this medically important plant species.

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Stepwise response of MeJA-induced genes and pathways in leaves of C. roseus

Cited by 5 publications

References 38 publications

Cell-type aware regulatory landscapes governing monoterpene indole alkaloid biosynthesis in the medicinal plantCatharanthus roseus

Cell-type aware regulatory landscapes governing monoterpene indole alkaloid biosynthesis in the medicinal plantCatharanthus roseus

Optimization of Flavonoid Production in Plant Cell Culture of Thevetia peruviana Elicited with Methyl Jasmonate and Salicylic Acid

Reference Genes Screening and Gene Expression Patterns Analysis Involved in Gelsenicine Biosynthesis under Different Hormone Treatments in Gelsemium elegans

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