Step Section Preparation of Transbronchial Lung Biopsy

Nagata, Nobuhiko; Honda, Hiroshi; Takayama, K.; Miyagawa, Yosuke; Shigematsu, Nobuaki

doi:10.1378/chest.100.4.959

Cited by 15 publications

(4 citation statements)

References 12 publications

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“…Likewise, Schick et al [ 17 ] used 3 to 10 levels each at 50 µ m apart and Nielsen et al [ 13 ] used 3 levels, each taken from 25%, 50%, and 75% into the block. Similar other studies conducted by Wu et al [ 16 ], Warnecke et al [ 21 ], Nagata et al [ 9 ], and Nash et al [ 15 ] have also used varied levels.…”

Section: Discussionsupporting

confidence: 57%

“…Deeper sections are frequently requested to enhance the sensitivity and diagnostic accuracy of the diseases [ 6 ]. Varieties of sections can be opted based on the need such as step section, multiple step section, deep cut, or better section [ 7 – 9 ].…”

Section: Introductionmentioning

confidence: 99%

“…Like in many hospitals, deeper sections are regularly practiced in our laboratory setup as well. Deeper sections have been found to be helpful in providing additional information during the final diagnosis of small biopsy specimens, thereby increasing the diagnostic accuracy leading to subsequent improvement in overall patient care [ 8 , 9 , 14 – 16 ]. This study has therefore been conducted to find the overall frequencies and levels of deeper section requested for small biopsy specimens of various organs and the factors warranting its need.…”

Section: Introductionmentioning

confidence: 99%

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Deeper Sections: Its Frequency and Diagnostic Utility in Histopathology of Noncutaneous Small Biopsy Specimen in a Tertiary Hospital in Nepal

Lama

Upadhyaya

Karki

et al. 2021

Advances in Medicine

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Background. Biopsy is an important tool for accurate diagnosis of disease in histopathology which can be examined at multiple levels during microscopic examination. The initial sections may not be representative of the entire biopsy, which leads to frequent request for deeper sections. This study assessed the frequency and diagnostic utility of deeper sections in noncutaneous small biopsy specimens at a tertiary hospital. Material and Methods. All the formalin fixed noncutaneous small biopsy specimens received were processed for grossing followed by tissue processing and embedding. The paraffin-embedded blocks were cut into 3–5 µm sections, fixed in a glass slide, deparaffinised, and then stained with hematoxylin and eosin (H&E) stain as per the standard protocol. Deeper sections were instructed based on microscopic findings of initial slides. The overall frequency of deeper section, its levels of cutting, tissue survival, and outcome were assessed and interpreted for the final diagnosis. Results. A total of 125 cases (26.9%) from 464 samples received were requested for deeper sections. The most frequent deeper sections were from cervix (72 cases) followed by stomach (18 cases) and endometrium (17 cases). The deeper sections were performed most frequently at 4 levels (17.8%) followed by 5 levels (14.8%) and 6 levels (13.3%). Deeper sections revealed nondiagnostic additional features in 28.2%, while 2.2% showed additional diagnostic features. Likewise, 2.2% had tissue segment loss. The overall mean level showing additional features was 6 levels. Tissue survival increased in 13% cases and 1.5% had decreased survival. The most common reason for requesting deeper section was to enhance sensitivity and diagnostic accuracy of disease. Conclusions. Deeper sections often contribute to final diagnosis. Therefore, deeper sections on biopsies that cannot readily be diagnosed on routine levels are recommended regardless of size of the lesion and there should be uniformity in the practice of deeper sections across the globe.

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Section: Discussionsupporting

confidence: 57%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Deeper Sections: Its Frequency and Diagnostic Utility in Histopathology of Noncutaneous Small Biopsy Specimen in a Tertiary Hospital in Nepal

Lama

Upadhyaya

Karki

et al. 2021

Advances in Medicine

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“…Several studies have been conducted to ascertain the optimal sections and/or levels in tiny biopsies. Several researchers [8,9] have shown that dividing into three or four levels is optimal, but they have found it challenging to generalize these findings. If you have a colorectal polyp, you should go to at least the third or sixth level [10].…”

Section: Introductionmentioning

confidence: 99%

The Frequency and Diagnostic Value of Deeper Sections in Histopathology of Non-Cutaneous Small Biopsy Specimens Obtained from a Tertiary Care Hospital

Khan¹,

Daud²,

Attaullah³

et al. 2022

PJMHS

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Objective: The purpose of this research is to ascertain the frequency of deeper sections and the diagnostic utility of these sections in non-cutaneous tiny biopsies obtained from a hospital setting. Study Design: Cross-sectional study Place and Duration: This study was carried out at Shaukat Khanum Memorial Hospital Peshawar, Jan 2022 to April 2022 Methods: In this study, there were a total of 100 patients, ranging in age from 16 to 50 years old. Patients who were hospitalized to the oral and maxillofacial department and who had deeper section procedures were considered for inclusion. Following the acquisition of informed and written consent, complete demographic information was obtained. The locations of organs and the levels at which leaks were found were documented for each and every instance. The mean, standard deviation, frequency, and percentages were the metrics that were used to evaluate the categorical variables. All of the data was analysed with SPSS version 22.0. Results: Among the deeper sections, we discovered that cervix cases accounted for 50%, stomach cases accounted for 25%, endometrial cases accounted for 15%, and colorectal cases accounted for 10%. Twenty-six instances 26%, had a deeper section done, with level 4 being the most prevalent. Although in most cases a diagnosis could be determined from the initial slide, in 55 of these cases (55%), further slides were reviewed to look for additional histological features. The goal was to either confirm the diagnosis obtained from the initial slide or raise confidence in that diagnosis. In 55 cases, 39 (70.9%) showed new pathological abnormalities in deeper sections, whereas 16 (29.1%) showed no change in histological features. Conclusion: A more in-depth examination is typically required in order to arrive at a certain diagnosis. Because of this, it is recommended that, regardless of the extent of the lesion, deeper sections be conducted on samples that cannot be consistently detected on ordinary levels, and this approach need to be standardized everywhere in the globe. Keywords: Histopathology, Deeper Sections, Non-cutaneous, Biopsy

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Collagen Vascular Diseases and Disorders of Connective Tissue

Butnor

Khoór

2008

Dail and Hammar’s Pulmonary Pathology

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Step Section Preparation of Transbronchial Lung Biopsy

Cited by 15 publications

References 12 publications

Deeper Sections: Its Frequency and Diagnostic Utility in Histopathology of Noncutaneous Small Biopsy Specimen in a Tertiary Hospital in Nepal

Deeper Sections: Its Frequency and Diagnostic Utility in Histopathology of Noncutaneous Small Biopsy Specimen in a Tertiary Hospital in Nepal

The Frequency and Diagnostic Value of Deeper Sections in Histopathology of Non-Cutaneous Small Biopsy Specimens Obtained from a Tertiary Care Hospital

Collagen Vascular Diseases and Disorders of Connective Tissue

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