Step-growth polymerization of traptavidin-DNA conjugates for plasmonic nanostructures

Kim, Young-Youb; Bang, Yongbin; Lee, Dayoung; Kang, Mingyu; Song, Yoon‐Kyu

doi:10.1016/j.cclet.2019.07.008

Cited by 6 publications

(7 citation statements)

References 35 publications

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“…The nanospheres have three reaction stages in the one-pot self-assembly system as shown in Figure A. First, biotinylated single-stranded DNA (M2) and half-complementary single-stranded DNA (M1) form a half double-stranded DNA (dsDNA) complex by step-growth polymerization . Subsequently, this dsDNA chain and streptavidin are combined to form hybrid nanospheres via biotin–streptavidin conjugation .…”

Section: Introductionmentioning

confidence: 99%

“…First, biotinylated single-stranded DNA (M2) and halfcomplementary single-stranded DNA (M1) form a half double-stranded DNA (dsDNA) complex by step-growth polymerization. 26 Subsequently, this dsDNA chain and streptavidin are combined to form hybrid nanospheres via biotin−streptavidin conjugation. 27 Extra streptavidin binding sites allow for further precise functionalization using other biotinylated functional materials (e.g., aptamer, targeting peptide, etc.).…”

Section: ■ Introductionmentioning

confidence: 99%

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Self-Assembled DNA–Protein Hybrid Nanospheres: Biocompatible Nano-Drug-Carriers for Targeted Cancer Therapy

Lee

Baek

Kim

et al. 2022

ACS Appl. Mater. Interfaces

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We developed hybrid nanospheres comprised of two of the most important biomolecules in nature, DNA and proteins, which have excellent biocompatibility, high drug payload capacity, in vivo imaging ability, and in vitro/in vivo cancer targeting capability. The synthesis can be done in a facile one-pot assembly system that includes three steps: step-growth polymerization of two DNA oligomers, addition of streptavidin to assemble spherical hybrid nanostructures, and functionalization of hybrid nanospheres with biotinylated aptamers. To test the feasibility of cancer targeting and drug-loading capacity of the hybrid nanospheres, MUC1-specific aptamers (MA3) were conjugated to nanosphere surfaces (apt-nanospheres), and doxorubicin (Dox) was loaded into nanospheres by DNA intercalation. The successful construction of nanospheres and apt-nanospheres was confirmed by agarose gel electrophoresis and dynamic light scattering (DLS). Their uniform spherical morphology was confirmed by transmission electron microscopy (TEM). Fluorescence spectra of nanospheres demonstrated high Dox-loading capability and slow-release characteristics. In vitro MUC1-specific binding of the apt-nanospheres was confirmed by flow cytometry and confocal microscopy. Dox-loaded apt-nanospheres significantly increased cytotoxicity of the MUC1-positive cancer cells due to aptamer-mediated selective internalization, as shown via cell viability assays. Apt-nanospheres could also be imaged in vivo through the synthesis of hybrid nanospheres using fluorescent dye-conjugated DNA strands. Finally, in vivo specific targeting ability of apt-nanospheres was confirmed in a MUC1-positive 4T1 tumor-bearing mouse model, whereas apt-nanospheres did not cause any sign of systemic toxicity in normal mice. Taken together, our self-assembled DNA–streptavidin hybrid nanospheres show promise as a biocompatible cancer targeting material for contemporary nanomedical technology.

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Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Self-Assembled DNA–Protein Hybrid Nanospheres: Biocompatible Nano-Drug-Carriers for Targeted Cancer Therapy

Lee

Baek

Kim

et al. 2022

ACS Appl. Mater. Interfaces

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“…Figure A shows the time evolution of the number-average degree of polymerization, X ̅ n = N 0 / N , where N is the total number of species of all sizes in the system, including monomers and chains, and N 0 is the number of monomers initially present. Figure A shows that X ̅ n scales linearly with time, which is characteristic of step-growth polymerization. ,, In the chaining of nanosized building blocks such as carbon nanotubes and gold nanorods, the step-growth polymerization mechanism − ,,,,− is most commonly observed, while only a few systems exhibit chain-growth or living polymerization. ,− In our system, each bead has comparable reactivity due to homogeneous surface functionalization and similar magnetic susceptibility. During the polymerization process, oligomers form first due to the assembly of monomers chemically linking via end-to-end interactions and then assemble into longer chains.…”

Section: Resultsmentioning

confidence: 72%

Chain Assembly Kinetics from Magnetic Colloidal Spheres

et al. 2022

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Magnetic colloidal chains are a microrobotic system with promising applications due to their versatility, biocompatibility, and ease of manipulation under magnetic fields. Their synthesis involves kinetic pathways that control chain quality, length, and flexibility, a process performed by first aligning superparamagnetic particles under a one-dimensional magnetic field and then chemically linking them using a four-armed maleimide-functionalized poly(ethylene glycol). Here, we systematically vary the concentration of the poly(ethylene glycol) linkers, the reaction temperature, and the magnetic field strength to study their impact on the physical properties of synthesized chains, including the chain length distribution, reaction temperature, and bending modulus. We find that this chain fabrication process resembles step-growth polymerization and can be accurately described by the Flory−Schulz model. Under optimized experimental conditions, we have successfully synthesized long flexible colloidal chains with a bending modulus, which is 4 orders of magnitude smaller than previous studies. Such flexible and long chains can be folded entirely into concentric rings and helices with multiple turns, demonstrating the potential for investigating the actuation, assembly, and folding behaviors of these colloidal polymer analogues.

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“…Such plasmonic nanostructures have the potential to boost optical signals for bioimaging applications. 33 2.2.3. DNA-Polymer Hybrid Nanostructures.…”

Section: Design Of Dna Nanostructuresmentioning

confidence: 99%

DNA-Based Nanostructured Platforms as Drug Delivery Systems

Kumar,

Jha,

Mishra

2024

Chem Bio Eng.

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DNA nanotechnology has recently provided a novel approach for developing safe, biocompatible, biodegradable, non-immunogenic, and non-toxic drug delivery systems. DNA nanostructures have numerous advantages for deployment as drug delivery platforms, owing to programmable assembly, ease of production, reproducibility, and precise control over size, shape, and function. DNA nanostructures can dramatically improve the delivery of poorly soluble drugs, decreasing cytotoxicity to normal tissues and improving therapeutic effectiveness. Using different conjugation methods, DNA nanostructures can be precisely integrated with a wide range of functional moieties, including proteins, peptides, aptamers, polymers, lipids, inorganic nanoparticles, and targeting groups, to enhance the nanostructure stability, extend circulation, and specify drug delivery. Smart DNA nanostructures with targeting ligands or a stimuli-responsive moiety specify therapeutic delivery to target, minimize drug loss attributable to prior drug release or off-target distribution, improve target accumulation, promote cellular internalization, bypass efflux pumps, and avoid adverse effects. Target-specific delivery by smart DNA nanostructures, in turn, maximizes drug concentration, reaching the target locations at a faster rate and preventing therapeutic failure while also lowering the dose needed for therapeutic effect. This Review provides an overview of DNA nanostructures for drug encapsulation and selective delivery to the desired sites. DNA nanostructures are a novel platform for drug delivery with improved performance that may be used to treat a variety of diseases.

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Step-growth polymerization of traptavidin-DNA conjugates for plasmonic nanostructures

Cited by 6 publications

References 35 publications

Self-Assembled DNA–Protein Hybrid Nanospheres: Biocompatible Nano-Drug-Carriers for Targeted Cancer Therapy

Self-Assembled DNA–Protein Hybrid Nanospheres: Biocompatible Nano-Drug-Carriers for Targeted Cancer Therapy

Chain Assembly Kinetics from Magnetic Colloidal Spheres

DNA-Based Nanostructured Platforms as Drug Delivery Systems

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